Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F73 I1 R1	31	34.8	1203930	99.1%	1193094	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,877,579	G→T	intergenic (‑114/+126)	yidB ← / ← gyrB	conserved protein YidB/DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,877,579	0	G	T	100.0%	109.6 / NA	33	intergenic (‑114/+126)	yidB/gyrB	conserved protein YidB/DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (17/16); total (17/16)

TTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAA  >  NC_000913/3877448‑3877709

ttCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGc                                                                                                                            <  1:411007/140‑1 (MQ=255)

   ttcaAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCgg                                                                                                                         >  2:114989/2‑140 (MQ=255)

         aaGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATgcgg                                                                                                                   >  1:594084/1‑140 (MQ=255)

                           cTGTAAAGCGATACGAGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGTCGAAGCTGATGTCCGCCCTTTTCTTATATCTTCTTGCAGGATCCGGCGTGAACGCCTGACCCgg                                                                                                 >  2:266365/1‑140 (MQ=255)

                            tGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCgtc                                                                                                <  2:313080/140‑3 (MQ=255)

                            tGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                >  2:525367/1‑140 (MQ=255)

                                     ttACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAt                                                                                       >  1:336371/1‑140 (MQ=255)

                                     ttACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAt                                                                                       >  2:567772/1‑140 (MQ=255)

                                          tgtTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGa                                                                                  <  2:407434/140‑1 (MQ=255)

                                          tgtTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGa                                                                                  <  2:53143/140‑1 (MQ=255)

                                          tgtTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGa                                                                                  <  2:306178/140‑1 (MQ=255)

                                          tgtTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGa                                                                                  <  1:253197/140‑1 (MQ=255)

                                          tgtTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGa                                                                                  <  1:225959/140‑1 (MQ=255)

                                                         tataGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTg                                                                   >  1:512846/1‑140 (MQ=255)

                                                                 tGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAttt                                                           >  2:506067/1‑140 (MQ=255)

                                                                   cGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTc                                                         >  2:534088/1‑140 (MQ=255)

                                                                    gggATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCg                                                        <  1:159418/140‑1 (MQ=255)

                                                                                gCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATaa                                            >  1:71043/1‑140 (MQ=255)

                                                                                gCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATaa                                            >  2:169899/1‑140 (MQ=255)

                                                                                 cGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAg                                           >  1:120935/1‑140 (MQ=255)

                                                                                    aTTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGt                                        >  2:211045/1‑140 (MQ=255)

                                                                                     ttGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTa                                       <  1:584360/140‑1 (MQ=255)

                                                                                      tGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAg                                      <  2:120935/140‑1 (MQ=255)

                                                                                                 aaaGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCa                           <  2:599400/140‑1 (MQ=255)

                                                                                                   aGGGCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACg                         <  1:60411/140‑1 (MQ=255)

                                                                                                     ggCGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCt                       >  2:166137/1‑140 (MQ=255)

                                                                                                       cGGAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCg                     >  2:244961/1‑140 (MQ=255)

                                                                                                        ggAGATGATCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGc                    <  2:561087/140‑1 (MQ=255)

                                                                                                             tgatCTCCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGCAGCGCATCAGGCACGCTCGCATGGt               <  1:114989/140‑1 (MQ=255)

                                                                                                                tctcCGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAg            <  2:71043/140‑1 (MQ=255)

                                                                                                                    cGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGc                    <  2:303700/128‑1 (MQ=255)

                                                                                                                    cGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGc                    >  1:303700/1‑128 (MQ=255)

                                                                                                                    cGCCCTTTTCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGcc        >  1:532487/1‑140 (MQ=255)

                                                                                                                          tttCTTATATCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGACTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTaaa  >  1:56106/1‑140 (MQ=255)

TTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAA  >  NC_000913/3877448‑3877709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A