Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F73 I1 R1	29	73.0	2473040	99.2%	2453255	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,946,923	G→T	noncoding (52/77 nt)	aspT →	tRNA‑Asp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,946,923	0	G	T	100.0%	130.2 / NA	40	noncoding (52/77 nt)	aspT	tRNA‑Asp
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (19/21); total (19/21)

GCCAGGCATCAAATTAAGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGA  >  NC_000913/3946811‑3947059

gCCAGGCATCAAATTAAGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCAcc                                                                                                               <  2:268460/140‑1 (MQ=11)

 ccAGGCATCAAATTAAGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCAccc                                                                                                              >  2:511014/1‑140 (MQ=14)

         cAAATTAAGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAg                                                                                                       >  1:82198/1‑139 (MQ=37)

         cAAATTAAGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAg                                                                                                       <  2:82198/139‑1 (MQ=37)

               aaGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTa                                                                                                <  1:1022444/140‑1 (MQ=255)

               aaGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTa                                                                                                <  2:416691/140‑1 (MQ=255)

               aaGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTa                                                                                                <  2:610130/140‑1 (MQ=255)

               aaGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTa                                                                                                >  1:713737/1‑140 (MQ=255)

                aGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCAcc                                                                                                               >  1:385415/1‑124 (MQ=11)

                aGCAGTAAGCCGGTCATAAAACCGGTGGTTGAAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTAAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCAcc                                                                                                               <  2:385415/124‑1 (MQ=2)

                 gCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGt                                                                                              <  1:511014/140‑1 (MQ=255)

                      aaGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAAt                                                                                         <  2:694300/140‑1 (MQ=255)

                      aaGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAAt                                                                                         <  2:1074250/140‑1 (MQ=255)

                            gTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTag                                                                                   >  2:92239/1‑140 (MQ=255)

                                   aaCCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCg                                                                            <  2:966376/140‑1 (MQ=255)

                                     ccGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATCCCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGt                                                                          <  1:648607/140‑1 (MQ=255)

                                       ggtggtTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGtct                                                                        >  1:6968/1‑140 (MQ=255)

                                        gtggtTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGtctc                                                                       >  1:991511/1‑140 (MQ=255)

                                              gTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAAc                                                                 <  2:713737/140‑1 (MQ=255)

                                                aaaaGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCg                                                               <  2:991511/140‑1 (MQ=255)

                                                     aaTTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGgtgt                                                          >  2:1224368/1‑140 (MQ=255)

                                                      aTTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGtt                                                         >  2:1055795/1‑140 (MQ=255)

                                                      aTTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGtt                                                         >  2:322335/1‑140 (MQ=255)

                                                             ggAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGtt                                                  <  1:1118614/140‑1 (MQ=255)

                                                                gCGGTAGTTCAGTCGGTTATAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGa                                               <  1:730502/140‑1 (MQ=255)

                                                                 cGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAg                                              <  2:1126671/140‑1 (MQ=255)

                                                                 cGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAg                                              <  2:1230802/140‑1 (MQ=255)

                                                                 cGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAg                                              >  1:1206274/1‑140 (MQ=255)

                                                                  ggTAGTTCGGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCCCCCTAATCAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGt                                             <  2:1074909/140‑1 (MQ=255)

                                                                         cAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCg                                      >  2:185686/1‑140 (MQ=255)

                                                                            tCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGccc                                   >  2:350669/1‑140 (MQ=255)

                                                                             cGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGcccc                                  <  1:421651/140‑1 (MQ=255)

                                                                                ttAGAATACCTGCCTGTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGc                               >  1:20968/1‑140 (MQ=255)

                                                                                         ctgcctgTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAatca                      >  1:768614/1‑140 (MQ=255)

                                                                                          tgcctgTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAatcat                     >  1:836693/1‑140 (MQ=255)

                                                                                          tgcctgTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAatcat                     >  1:112536/1‑140 (MQ=255)

                                                                                            cctgTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATcc                   <  2:1188717/140‑1 (MQ=255)

                                                                                              tgTCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCtt                 >  2:619330/1‑140 (MQ=255)

                                                                                                tCACGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAg               >  2:1117457/1‑140 (MQ=255)

                                                                                                  aCGCAGGGGGTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCg             <  2:112536/140‑1 (MQ=255)

                                                                                                        ggggTCGCTGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCt       <  1:619330/140‑1 (MQ=255)

                                                                                                             cgctGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa  >  1:1194982/1‑140 (MQ=255)

GCCAGGCATCAAATTAAGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGA  >  NC_000913/3946811‑3947059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A