breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 79,171 C→A D95Y (GAC→TAC)  leuD ← 3‑isopropylmalate dehydratase subunit LeuD
RA 175,412 G→T P102P (CCG→CCT clcA → chloride:H(+) antiporter ClcA
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 285,577 G→T P61P (CCG→CCT yagG → putative D‑xylonate transporter YagG
RA 299,332 C→A G535W (GGG→TGG)  paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 751,341 T→G N277H (AAT→CAT)  ybgQ ← putative fimbrial usher protein YbgQ
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,126,617 G→T R154L (CGC→CTC)  yceH → DUF480 domain‑containing protein YceH
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,705,169 A→C intergenic (+5/‑81) cnu → / → ydgK H‑NS‑ and StpA‑binding protein/conserved inner membrane protein YdgK
RA 1,746,461 A→G noncoding (27/77 nt) valV → tRNA‑Val
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,979,551 C→A intergenic (‑336/‑202) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,222,536 G→T A118S (GCC→TCC)  dld → D‑lactate dehydrogenase
RA 2,304,259 G→T A119S (GCG→TCG)  eco → serine protease inhibitor ecotin
RA 2,552,129 G→T R4S (CGC→AGC)  ypeA ← putative acetyltransferase YpeA
RA 3,220,829 C→A R446S (CGT→AGT)  patA → putrescine aminotransferase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,859,407 C→A G237V (GGC→GTC)  yidK ← putative transporter YidK
RA 3,946,923 G→T noncoding (52/77 nt) aspT → tRNA‑Asp
RA 3,983,659 C→A P234P (CCC→CCA aslB → putative anaerobic sulfatase maturation enzyme AslB
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,618,191 C→A intergenic (+89/‑38) deoC → / → deoA deoxyribose‑phosphate aldolase/thymidine phosphorylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102957 3104051 1095 18 [17] [14] 19 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424234 3424530–3424240 7–761 18 [14] [17] 19 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =16 (0.220)77 (1.200) 47/244 0.2 84.1% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 15 (0.230)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780516 (0.220)52 (0.810) 40/244 0.4 78.1% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 15 (0.230)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 3363001 =113 (1.550)115 (1.590) 54/274 0.2 66.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)82 (1.130) 54/276 0.2 96.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA