Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F73 I1 R1	29	73.0	2473040	99.2%	2453255	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,983,659	C→A	P234P (CCC→CCA)	aslB →	putative anaerobic sulfatase maturation enzyme AslB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,983,659	0	C	A	100.0%	202.9 / NA	57	P234P (CCC→CCA)	aslB	putative anaerobic sulfatase maturation enzyme AslB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (29/28); total (29/28)

TGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATC  >  NC_000913/3983522‑3983789

tgCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAga                                                                                                                                  >  2:1226855/1‑140 (MQ=255)

  cGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAgagg                                                                                                                                >  1:135762/1‑140 (MQ=255)

    aTGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAgaggag                                                                                                                              <  1:679792/140‑1 (MQ=255)

              cGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGt                                                                                                                    <  1:1100700/140‑1 (MQ=255)

               gAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGtt                                                                                                                   >  2:1195637/1‑140 (MQ=255)

               gAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGtt                                                                                                                   >  2:706305/1‑140 (MQ=255)

                aaTTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGttt                                                                                                                  <  2:474617/140‑1 (MQ=255)

                        cAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGc                                                                                                          <  1:185804/140‑1 (MQ=255)

                          gTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGa                                                                                                        <  2:135762/140‑1 (MQ=255)

                           tttATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGat                                                                                                       >  2:851239/1‑140 (MQ=255)

                               ttCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTc                                                                                                   <  1:1195637/140‑1 (MQ=255)

                               ttCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTc                                                                                                   <  2:504023/140‑1 (MQ=255)

                               ttCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTc                                                                                                   <  2:442605/140‑1 (MQ=255)

                                tCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCg                                                                                                  <  1:155532/140‑1 (MQ=255)

                                    gtggtCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCa                                                                                              <  2:404657/140‑1 (MQ=255)

                                     tggtCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCAc                                                                                             >  2:515264/1‑140 (MQ=255)

                                     tggtCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCAc                                                                                             >  2:1142384/1‑140 (MQ=255)

                                       gtCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTg                                                                                           >  1:155782/1‑140 (MQ=255)

                                       gtCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGATCGGTGAGCTTCTGGTGGCGATATTCGACCACTg                                                                                           >  1:1005461/1‑140 (MQ=255)

                                         cGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGa                                                                                         >  2:178761/1‑140 (MQ=255)

                                                 tGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGAcc                                                                                               <  1:1078237/126‑1 (MQ=255)

                                                 tGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGAcc                                                                                               >  2:1078237/1‑126 (MQ=255)

                                                           aacaacTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCa                                                                       <  2:279001/140‑1 (MQ=255)

                                                                   cccGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTc                                                               <  2:79810/140‑1 (MQ=255)

                                                                     cgcgATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGt                                                             >  1:1183132/1‑140 (MQ=255)

                                                                       cgATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGtga                                                           <  1:1226855/140‑1 (MQ=255)

                                                                        gATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTTGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGAAAGATTTTCGtgat                                                          <  2:1005461/140‑1 (MQ=255)

                                                                        gATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGtgat                                                          <  2:1183132/140‑1 (MQ=255)

                                                                           ggACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGaa                                                       <  1:515264/140‑1 (MQ=255)

                                                                             aCTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAata                                                     >  1:607450/1‑140 (MQ=255)

                                                                                   gTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAAt                                               <  2:155782/140‑1 (MQ=255)

                                                                                       cATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGc                                           >  1:467368/1‑140 (MQ=255)

                                                                                          gcgcCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGtt                                        <  1:851239/140‑1 (MQ=255)

                                                                                           cgcCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGttt                                       >  2:640875/1‑140 (MQ=255)

                                                                                            gcCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTg                                      <  1:1142384/140‑1 (MQ=255)

                                                                                            gcCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTg                                      <  1:706305/140‑1 (MQ=255)

                                                                                             ccTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGc                                     >  1:880240/1‑140 (MQ=255)

                                                                                              cTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGcc                                    <  2:861951/140‑1 (MQ=255)

                                                                                                 gTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAAt                                 >  1:241378/1‑140 (MQ=255)

                                                                                                          aGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGt                        >  1:1098867/1‑140 (MQ=255)

                                                                                                          aGGGTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGt                        >  2:954234/1‑140 (MQ=255)

                                                                                                           gggTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTg                       >  1:245083/1‑140 (MQ=255)

                                                                                                           gggTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTg                       >  1:206573/1‑140 (MQ=255)

                                                                                                            ggTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTgc                      >  2:154312/1‑140 (MQ=255)

                                                                                                            ggTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTgc                      <  1:460219/140‑1 (MQ=255)

                                                                                                            ggTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTgc                      >  2:1224652/1‑140 (MQ=255)

                                                                                                            ggTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTgc                      >  2:915658/1‑140 (MQ=255)

                                                                                                            ggTGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTgc                      >  2:711708/1‑140 (MQ=255)

                                                                                                              tGAGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTgcgc                    <  2:254898/140‑1 (MQ=255)

                                                                                                                aGCTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCg                  >  1:996426/1‑140 (MQ=255)

                                                                                                                  cTAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGACGGTGCGCCggg                <  2:241378/140‑1 (MQ=255)

                                                                                                                   tAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGt               <  2:245083/140‑1 (MQ=255)

                                                                                                                   tAACGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGt               <  1:954234/140‑1 (MQ=255)

                                                                                                                     aCGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGc             <  1:185390/140‑1 (MQ=255)

                                                                                                                      cGGAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCg            >  2:1223906/1‑140 (MQ=255)

                                                                                                                        gAATGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGt          <  1:836949/140‑1 (MQ=255)

                                                                                                                          aTGGTCGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGttt        >  1:914670/1‑140 (MQ=255)

                                                                                                                               cGGTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCcat   >  2:115949/1‑140 (MQ=255)

                                                                                                                                ggTGCGCCCAGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCcatc  <  2:914670/140‑1 (MQ=255)

TGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATC  >  NC_000913/3983522‑3983789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A