Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F73 I1 R1	26	74.0	2563470	99.3%	2545525	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,049,975	C→A	A231A (GCG→GCT)	gcvT ←	aminomethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,049,975	0	C	A	98.5%	233.6 / ‑2.8	67	A231A (GCG→GCT)	gcvT	aminomethyltransferase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (33/33); total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

CCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCCGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAGTGG  >  NC_000913/3049838‑3050111

ccATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGc                                                                                                                                        >  1:45884/1‑140 (MQ=255)

   tGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTc                                                                                                                                     >  1:1000692/1‑140 (MQ=255)

   tGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTc                                                                                                                                     >  1:70809/1‑140 (MQ=255)

   tGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTc                                                                                                                                     >  1:638636/1‑140 (MQ=255)

    gCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTcc                                                                                                                                    >  1:555157/1‑140 (MQ=255)

       cACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGa                                                                                                                                 <  1:1128004/140‑1 (MQ=255)

       cACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGa                                                                                                                                 <  2:855274/140‑1 (MQ=255)

            tGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAg                                                                                                                            <  2:463878/140‑1 (MQ=255)

              cACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCg                                                                                                                          >  1:1223329/1‑140 (MQ=255)

                   ccAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCa                                                                                                                     >  2:1170207/1‑140 (MQ=255)

                        gCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCAcgcgc                                                                                                                <  1:352752/140‑1 (MQ=255)

                           tCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGTTGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGcc                                                                                                             >  2:265791/1‑140 (MQ=255)

                           tCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGcc                                                                                                             >  2:757586/1‑140 (MQ=255)

                           tCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGcc                                                                                                             >  1:343450/1‑140 (MQ=255)

                            cACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGccc                                                                                                            <  1:179311/140‑1 (MQ=255)

                             aCGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACgcgcgcgca                                                                                                           <  2:70809/140‑4 (MQ=255)

                                 ccGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGcc                                                                                                       >  1:13653/1‑140 (MQ=255)

                                  cGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCca                                                                                                      >  2:276190/1‑140 (MQ=255)

                                    aTAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCcaca                                                                                                    <  2:5513/140‑1 (MQ=255)

                                    aTAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCcaca                                                                                                    >  1:861423/1‑140 (MQ=255)

                                    aTAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCcaca                                                                                                    <  2:570885/140‑1 (MQ=255)

                                     tAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACAt                                                                                                   >  1:1119948/1‑140 (MQ=255)

                                       aaGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATgg                                                                                                 <  2:555157/140‑1 (MQ=255)

                                            aCGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTaa                                                                                            >  2:770994/1‑140 (MQ=255)

                                               aTCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAacac                                                                                         <  2:643228/140‑1 (MQ=255)

                                               aTCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAacac                                                                                         <  2:1035688/140‑1 (MQ=255)

                                               aTCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAacac                                                                                         <  1:746768/140‑1 (MQ=255)

                                               aTCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAacac                                                                                         <  2:557207/140‑1 (MQ=255)

                                                            cAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAg                                                                            >  2:1258633/1‑140 (MQ=255)

                                                              ggCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAgcg                                                                          <  2:799346/140‑1 (MQ=255)

                                                                  aTGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACg                                                                      >  1:512004/1‑140 (MQ=255)

                                                                    ggTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGcc                                                                    >  1:733715/1‑140 (MQ=255)

                                                                       ccAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGa                                                                 >  1:1200013/1‑140 (MQ=255)

                                                                              aTGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGc                                                          <  1:770994/140‑1 (MQ=255)

                                                                               tGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGcc                                                         >  2:165313/1‑140 (MQ=255)

                                                                                gTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCg                                                        >  1:1000885/1‑140 (MQ=255)

                                                                                   ggcggcTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCtt                                                     <  1:731009/140‑1 (MQ=255)

                                                                                   ggcggcTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCtt                                                     <  2:512004/140‑1 (MQ=255)

                                                                                   ggcggcTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCtt                                                     <  1:168878/140‑1 (MQ=255)

                                                                                   ggcggcTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCtt                                                     <  2:13653/140‑1 (MQ=255)

                                                                                     cggcTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAATACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCtttt                                                   >  1:1130239/1‑140 (MQ=255)

                                                                                       gcTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTc                                                 >  2:996259/1‑140 (MQ=255)

                                                                                             ggAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTggg                                           <  1:1253147/140‑1 (MQ=255)

                                                                                                    tGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCa                                    <  2:1200013/140‑1 (MQ=255)

                                                                                                    tGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCa                                    <  2:736910/140‑1 (MQ=255)

                                                                                                        ttCGTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAAttt                                >  2:716618/1‑140 (MQ=255)

                                                                                                           gTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCAt                             >  2:526991/1‑140 (MQ=255)

                                                                                                           gTCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCAt                             >  2:575805/1‑140 (MQ=255)

                                                                                                            tCCATCTCCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGACACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATa                            <  2:854580/140‑1 (MQ=255)

                                                                                                                tctcCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGccc                        >  2:170858/1‑140 (MQ=255)

                                                                                                                 ctcCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAgcg                                      >  2:943266/1‑125 (MQ=255)

                                                                                                                 ctcCTGACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAgcg                                      <  1:943266/125‑1 (MQ=255)

                                                                                                                    ttGACCATAAAGATTCATGCCAGCTTCCTGACGCAGCGTGCCACGCGCGCACAAGCCACATGCCTTAACACCCGCTTCCCCCAGCGCACGCCAGAAATCGGCCGTTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCtt                    <  2:861423/139‑1 (MQ=255)

                                                                                                                      gACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCa                  <  2:917769/140‑1 (MQ=255)

                                                                                                                      gACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCa                  <  2:1158450/140‑1 (MQ=255)

                                                                                                                      gACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCa                  <  2:1119948/140‑1 (MQ=255)

                                                                                                                      gACCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCa                  <  1:1170207/140‑1 (MQ=255)

                                                                                                                       aCCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCAc                 >  1:1065426/1‑140 (MQ=255)

                                                                                                                       aCCATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCAc                 <  2:343450/140‑1 (MQ=255)

                                                                                                                         cATAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCg               >  1:649609/1‑140 (MQ=255)

                                                                                                                           tAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGt             <  1:265791/140‑1 (MQ=255)

                                                                                                                           tAAAGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGt             <  1:663180/140‑1 (MQ=255)

                                                                                                                            aaaGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGta            >  2:758423/1‑140 (MQ=255)

                                                                                                                            aaaGATTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGta            >  1:975687/1‑140 (MQ=255)

                                                                                                                                aTTCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAATACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAAc        <  2:1130239/140‑1 (MQ=255)

                                                                                                                                 ttCATGCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAAcc       >  2:27702/1‑140 (MQ=255)

                                                                                                                                   cATGCCCGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAg     >  1:654625/1‑140 (MQ=255)

                                                                                                                                      gCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAgtgg  <  2:1000692/140‑1 (MQ=255)

                                                                                                                                      gCCAGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAgtgg  <  2:223134/140‑1 (MQ=255)

CCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTCCATCTCCTGACCATAAAGATTCATGCCCGCTTCCAGACGCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAGTGG  >  NC_000913/3049838‑3050111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A