Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F73 I1 R1 26 44.8 1518598 99.2% 1506449 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,372,314 C→A R469R (CGC→CGA)  arnT → lipid IVA 4‑amino‑4‑deoxy‑L‑arabinosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,372,3140CA96.5% 194.2 / ‑0.5 57R469R (CGC→CGA) arnTlipid IVA 4‑amino‑4‑deoxy‑L‑arabinosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (32/23);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATTCCTGACAGAGTTATGGAAGGAAAACATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGCGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAGGGGATTATT  >  NC_000913/2372178‑2372446
                                                                                                                                        |                                                                                                                                    
cAATTCCTGACAGAGTTATGGAAGGAAAACATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACgaga                                                                                                                                    >  1:85248/1‑139 (MQ=255)
    tCCTGACAGAGTTATGGAAGGAAAACATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGAca                                                                                                                               <  2:120230/140‑1 (MQ=255)
              gTTATGGAAGGAAAACATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTa                                                                                                                     >  1:631969/1‑140 (MQ=255)
                tATGGAAGGAAAACATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATc                                                                                                                   >  1:431083/1‑140 (MQ=255)
                   ggaaggaaAACATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGcc                                                                                                                >  2:175873/1‑140 (MQ=255)
                      aggaaAACATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGa                                                                                                             <  2:631969/140‑1 (MQ=255)
                         aaaaCATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAg                                                                                                          >  2:624123/1‑140 (MQ=255)
                           aaCATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGt                                                                                                        <  2:753536/140‑1 (MQ=255)
                            aCATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTg                                                                                                       <  1:175873/140‑1 (MQ=255)
                               tCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGt                                                                                                    >  1:360831/1‑140 (MQ=255)
                                 cGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGt                                                                                                        >  2:32819/1‑134 (MQ=255)
                                 cGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGt                                                                                                        <  1:32819/134‑1 (MQ=255)
                                  gCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGa                                                                                                 >  1:684109/1‑140 (MQ=255)
                                    aaTTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAgg                                                                                                         >  2:501197/1‑130 (MQ=255)
                                    aaTTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAgg                                                                                                         <  1:501197/130‑1 (MQ=255)
                                     aTTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAAt                                                                                              <  1:624123/140‑1 (MQ=255)
                                             tCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGAGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGCGATGACCTCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCtt                                                                                      >  1:457929/1‑140 (MQ=255)
                                                agaTGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAAt                                                                                   >  1:38933/1‑140 (MQ=255)
                                                                     aGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGgaga                                                              >  2:352099/1‑140 (MQ=255)
                                                                              gCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGCGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGc                                                     <  2:457929/140‑1 (MQ=255)
                                                                                    ttCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGtgat                                               <  2:431083/140‑1 (MQ=255)
                                                                                     tCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGtgatg                                              >  1:670089/1‑140 (MQ=255)
                                                                                         aCTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGtt                                          >  2:151662/1‑140 (MQ=255)
                                                                                         aCTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGtt                                          >  1:702878/1‑140 (MQ=255)
                                                                                            gATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGGTTTGTCAGCGGTGATGAGTTCGc                                       >  1:26810/1‑140 (MQ=255)
                                                                                            gATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGc                                       >  2:695129/1‑140 (MQ=255)
                                                                                            gATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGc                                       >  1:264671/1‑140 (MQ=255)
                                                                                              tAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCaa                                     >  2:375932/1‑140 (MQ=255)
                                                                                              tAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCaa                                     >  1:754659/1‑140 (MQ=255)
                                                                                               aGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCaaa                                    >  2:570741/1‑140 (MQ=255)
                                                                                               aGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCaaa                                    >  2:180581/1‑140 (MQ=255)
                                                                                               aGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCaaa                                    >  1:490644/1‑140 (MQ=255)
                                                                                               aGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCaaa                                    >  1:42617/1‑140 (MQ=255)
                                                                                                       tgtTGCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTa                            >  2:435107/1‑140 (MQ=255)
                                                                                                           gCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCaaa                                    <  1:79849/128‑1 (MQ=255)
                                                                                                           gCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCaaa                                    >  2:79849/1‑128 (MQ=255)
                                                                                                           gCCGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCACAAACTGGCTTAATCa                        <  2:670089/140‑1 (MQ=255)
                                                                                                             cGCAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAAc                      <  1:695129/140‑1 (MQ=255)
                                                                                                               cAGGTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACAt                    >  1:418333/1‑140 (MQ=255)
                                                                                                                 ggTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGAATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCg                  >  1:116265/1‑140 (MQ=255)
                                                                                                                  gTCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGt                 <  1:151662/140‑1 (MQ=255)
                                                                                                                   tCTGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTc                <  2:360831/140‑1 (MQ=255)
                                                                                                                     tGGCATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCag              <  2:702878/140‑1 (MQ=255)
                                                                                                                        cATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCaggag           <  1:180581/140‑1 (MQ=255)
                                                                                                                        cATGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCaggag           <  2:116265/140‑1 (MQ=255)
                                                                                                                          tGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAggg         <  2:42617/140‑1 (MQ=255)
                                                                                                                          tGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAggg         <  2:264671/140‑1 (MQ=255)
                                                                                                                          tGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAggg         <  1:375932/140‑1 (MQ=255)
                                                                                                                          tGGAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAggg         <  2:418333/140‑1 (MQ=255)
                                                                                                                            gAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAggg         <  2:624868/138‑1 (MQ=255)
                                                                                                                            gAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAggg         >  1:624868/1‑138 (MQ=255)
                                                                                                                            gAGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAGGGGa       >  2:341504/1‑140 (MQ=255)
                                                                                                                             aGCCTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAGGGGat      >  2:671974/1‑140 (MQ=255)
                                                                                                                                cTGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGGTTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAGGGGattat   <  2:26810/140‑1 (MQ=255)
                                                                                                                                 tGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAggg         <  2:628721/133‑1 (MQ=255)
                                                                                                                                 tGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAggg         >  1:628721/1‑133 (MQ=255)
                                                                                                                                 tGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAGGGGattatt  >  1:566760/1‑140 (MQ=255)
                                                                                                                                 tGCAACGAGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAGGGGattatt  >  1:172225/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                    
CAATTCCTGACAGAGTTATGGAAGGAAAACATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGCGATGACATCATCATGTATCGCCAGACAGGTGAGTTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACATCGTCAGGAGGGGATTATT  >  NC_000913/2372178‑2372446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A