Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F73 I1 R1	26	44.8	1518598	99.2%	1506449	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,652,671	G→T	D60Y (GAT→TAT)	sseA →	3‑mercaptopyruvate sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,652,671	0	G	T	100.0%	141.1 / NA	42	D60Y (GAT→TAT)	sseA	3‑mercaptopyruvate sulfurtransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (25/17); total (25/17)

CATATTGATGACCCGGAAATTCAGATTATCGATGCCCGCATGGCGTCGCCTGGACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTGATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGC  >  NC_000913/2652536‑2652804

cATATTGATGACCCGGAAATTCAGATTATCGATGCCCGCATGGCGTCGCCTGGACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATca                                                                                                                                   <  1:21549/140‑1 (MQ=255)

          aCCCGGAAATTCAGATTATCGATGCCCGCATGGCGTCGCCTGGACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCg                                                                                                                         >  2:478486/1‑140 (MQ=255)

           cccGGAAATTCAGATTATCGATGCCCGCATGGCGTCGCCTGGACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGc                                                                                                                        >  2:735555/1‑140 (MQ=255)

              ggAAATTCAGATTATCGATGCCCGCATGGCGTCGCCTGGACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTc                                                                                                                     >  2:426986/1‑140 (MQ=255)

               gAAATTCAGATTATCGATGCCCGCATGGCGTCGCCTGGACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTcc                                                                                                                    >  2:309878/1‑140 (MQ=255)

                            tCGATGCCCGCATGGCGTCGCCTGGACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCTGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCTGCACATGCTGCcg                                                                                                       >  2:239690/1‑140 (MQ=255)

                            tCGATGCCCGCATGGCGTCGCCTGGACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCcg                                                                                                       >  1:139312/1‑140 (MQ=255)

                                tGCCCGCATGGCGTCGCCTGGACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGcc                                                                                                   >  1:631463/1‑140 (MQ=255)

                                    cGCATGGCGTCGCCTGGACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGa                                                                                               >  1:485890/1‑140 (MQ=255)

                                      cATGGCGTCGCCTGGACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGaaa                                                                                             >  1:414310/1‑140 (MQ=255)

                                                    gACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCg                                                                               <  2:414310/140‑1 (MQ=255)

                                                    gACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCg                                                                               <  1:426986/140‑1 (MQ=255)

                                                         gaggaTCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCg                                                                          >  2:205648/1‑140 (MQ=255)

                                                         gaggaTCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCg                                                                          <  1:735555/140‑1 (MQ=255)

                                                          aggaTCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCg                                                                               >  2:548531/1‑134 (MQ=255)

                                                          aggaTCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCg                                                                               <  1:548531/134‑1 (MQ=255)

                                                                   aCGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATg                                                                            <  1:214173/128‑1 (MQ=255)

                                                                   aCGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATg                                                                            >  2:214173/1‑128 (MQ=255)

                                                                    cGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCTGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCTGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCg                                                               <  1:239690/140‑1 (MQ=255)

                                                                         cTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAAc                                                          >  2:111325/1‑140 (MQ=255)

                                                                          tCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAAcc                                                         <  2:373208/140‑1 (MQ=255)

                                                                               aGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGAt                                                    <  2:631463/140‑1 (MQ=255)

                                                                               aGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGAt                                                    >  2:175688/1‑140 (MQ=255)

                                                                                gTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATa                                                   <  1:175688/140‑1 (MQ=255)

                                                                                 tATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAAcc                                                         >  1:34261/1‑133 (MQ=255)

                                                                                 tATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAAcc                                                         <  2:34261/133‑1 (MQ=255)

                                                                                   tCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGc                                                <  1:111325/140‑1 (MQ=255)

                                                                                      gAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCAcc                                             <  1:205648/140‑1 (MQ=255)

                                                                                        aTGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTg                                           >  1:75418/1‑140 (MQ=255)

                                                                                        aTGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTg                                           >  2:79609/1‑140 (MQ=255)

                                                                                         tGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGa                                          <  2:139312/140‑1 (MQ=255)

                                                                                             cATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGt                                      >  1:652746/1‑140 (MQ=255)

                                                                                                 ttCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGTCt                                    <  2:173997/138‑1 (MQ=255)

                                                                                                 ttCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGTCt                                    >  1:173997/1‑138 (MQ=255)

                                                                                                      ggCGCAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGTCTATGACGa                             >  2:512915/1‑140 (MQ=255)

                                                                                                       gcgcAGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGGCTATGACGaa                            >  1:363243/1‑140 (MQ=255)

                                                                                                           aGTGTTTTTTGATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGTCTATGACGAAGGTa                        >  1:634845/1‑140 (MQ=255)

                                                                                                                     gATATCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTtctc              <  1:512915/140‑1 (MQ=255)

                                                                                                                       tatCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAg            >  2:428634/1‑140 (MQ=255)

                                                                                                                         tCGAAGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGcc          >  2:397115/1‑140 (MQ=255)

                                                                                                                            aaGCGCTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCa       <  1:309878/140‑1 (MQ=255)

                                                                                                                                 cTTTCTTATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGc  >  2:122663/1‑140 (MQ=255)

CATATTGATGACCCGGAAATTCAGATTATCGATGCCCGCATGGCGTCGCCTGGACAGGAGGATCGTAACGTTGCTCAGGAGTATCTGAATGGACATATTCCCGGCGCAGTGTTTTTTGATATCGAAGCGCTTTCTGATCACACTTCCCCGCTTCCGCACATGCTGCCGCGCCCGGAAACGTTCGCCGTGGCGATGCGTGAATTAGGCGTTAACCAGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGC  >  NC_000913/2652536‑2652804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A