Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F73 I1 R1 26 44.8 1518598 99.2% 1506449 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,006,039 C→A intergenic (‑253/‑223) ygeV ← / → ygeW putative sigma(54)‑dependent transcriptional regulator YgeV/putative carbamoyltransferase YgeW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,006,0390CA100.0% 115.9 / NA 35intergenic (‑253/‑223)ygeV/ygeWputative sigma(54)‑dependent transcriptional regulator YgeV/putative carbamoyltransferase YgeW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/16);  total (19/16)

ATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTT‑AGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTC  >  NC_000913/3005916‑3006175
                                                                                                                            |                                                                                                                                        
aTTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTTAAAAGAAAAAGACCGCCCCCTTTTCTTATTTTTTATACTT‑AGCAGCAAAAAAATAACGCGATAAAAAAAACCaaa                                                                                                                          >  2:233418/1‑140 (MQ=255)
aTTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCaaa                                                                                                                          <  1:632948/140‑1 (MQ=255)
               tcatctttCTATATCAATATGAGAATTACGGGGGTGAGTTTAGCAAAATGAAGAAACATAGACGGCTCTTTTATCTTATTTCTGATCCTT‑AGCAGCAAATAAATAGCGAGATAAAAAGAGCCAATCGTTTACGAAttttg                                                                                                           <  2:723304/135‑2 (MQ=255)
                    tttCTATATCAATATGAGAATTACGGCGGTGAGGTTATCAAAATGAAGAGAGATAACCTGCCCCTTTATCTTTTTTTTTATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAACCAAACGTTTTTGTATTTTacaaaa                                                                                                      >  2:80506/1‑139 (MQ=255)
                               atatGAGAATTACGGCGGTGAGTTTTTCAAAATGAAGAAAGATAAGCTGCCCCTTTTTCTTATTTCTTATACTT‑AGCAGCAAAAAAAAAAAAAGAAAAAAAAAACCAAACGTTTTCGTAATTTACAAAAAAACaaaaaat                                                                                           >  2:334679/1‑137 (MQ=255)
                                tatGAGAATTACGGCGGTGAGGTTATCAAAATGAAAAGAAAAAACCAGCCCCCTTTTTCTATTTCTGGGACTTAATCAGC‑AATAAATAAAAAAATAAAAAAAACCAAAACGTTTCGCATTTTACAAAAAACCAGAATCtt                                                                                          >  2:480975/1‑139 (MQ=255)
                                    agaATTACGGCGGTGAGGTTTTCAAAATGAAGAGAGATAGCCTGCCCCTTTTTTTTTTTTTTGATACTT‑AGCAGCAAAAAAATAACGAGATAAAAAAAACCAAACGTTTTTGTATTTTTTAAAAAACCAAAAACTGGCaa                                                                                      >  2:744017/1‑139 (MQ=255)
                                        ttACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTTTGATACTT‑AGCAGCAAATAAAAAACGAGATAAAAAAAGCCAAAAGTTTTTGTATTTTACAAACAACCCGAAGCTAGCATCaaa                                                                                  >  2:386031/1‑139 (MQ=255)
                                        ttACGGCGGTGAGGTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTTTTTCTGATACTT‑AGCAGCAAATAAATAAAGAGATAAAAAAAACCAAAAGTTTTTGTATTTTACAAAAAACCAGAAGCTGGCATCAAt                                                                                  >  2:732173/1‑140 (MQ=255)
                                          aCGGCGGTGAGTTTTTTAAACTGAAGAGAGCTAGCCTGCCCCTTTATCTTTTTTCTGATACTT‑AGCAGCAAAAAAAAAAAGAGAAAAAAAAAACCAAACGTTTTTGTATTTTTCAAAAAACCAGAAACTGGCATCAAttt                                                                                >  2:240328/1‑140 (MQ=255)
                                          aCGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAAttt                                                                                >  1:593237/1‑140 (MQ=255)
                                               ggTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGAt                                                                           <  2:593237/140‑1 (MQ=255)
                                                     tttATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAAccc                                                                     <  1:334679/140‑1 (MQ=255)
                                                                      agaTAGCCTGCCCCTTTATCTTATTTCTGATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACAt                                                              >  2:442114/1‑130 (MQ=255)
                                                                      agaTAGCCTGCCCCTTTATCTTATTTCTGATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACAt                                                              <  1:442114/130‑1 (MQ=255)
                                                                       gaTAGCCTGCCCCTTTATCTTATTTCTGATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCaaa                                                   >  2:505421/1‑140 (MQ=255)
                                                                        aTAGCCTGCCCCTTTATCTTATTTCTGATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAAt                                                  <  1:732173/140‑1 (MQ=255)
                                                                                ccccTTTATCTTATTTCTGATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCttt                                          >  2:434873/1‑140 (MQ=255)
                                                                                      tATCTTATTTCTGATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGc                                    >  2:603176/1‑140 (MQ=255)
                                                                                          ttATTTCTGATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCcgcg                                <  1:744017/140‑1 (MQ=255)
                                                                                              ttCTGATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGa                            <  1:603176/140‑1 (MQ=255)
                                                                                                  gATACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAAt                        >  2:717012/1‑140 (MQ=255)
                                                                                                   aTACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAAtt                       >  2:461092/1‑140 (MQ=255)
                                                                                                   aTACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAAtt                       >  2:308626/1‑140 (MQ=255)
                                                                                                    tACTT‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTc                      <  1:38049/140‑1 (MQ=255)
                                                                                                        t‑AGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGc                  <  1:308626/140‑1 (MQ=255)
                                                                                                          agcagcAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCgg                             <  1:534685/128‑1 (MQ=255)
                                                                                                          agcagcAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCgg                             >  2:534685/1‑128 (MQ=255)
                                                                                                          agcagcAAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGca                 >  2:362598/1‑140 (MQ=255)
                                                                                                            cagcaAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGcaca               <  1:362598/140‑1 (MQ=255)
                                                                                                              gcaAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACt             >  1:587635/1‑140 (MQ=255)
                                                                                                               caAATAAATAACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAGCCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACtt            <  1:240328/140‑1 (MQ=255)
                                                                                                                 aataaataACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGAt                           >  1:165419/1‑123 (MQ=255)
                                                                                                                 aataaataACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGAt                           <  2:165419/123‑1 (MQ=255)
                                                                                                                 aataaataACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTAt          >  2:177794/1‑140 (MQ=255)
                                                                                                                 aataaataACGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTAt          >  1:31849/1‑140 (MQ=255)
                                                                                                                  ataaataaCGAGATAAAAAAAGCCAAACGTTTTCGGATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGGTCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTAtt         >  2:545394/1‑140 (MQ=255)
                                                                                                                    aaataaCGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGt       <  2:382220/140‑1 (MQ=255)
                                                                                                                    aaataaCGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGt       <  2:635132/140‑1 (MQ=255)
                                                                                                                         aCGAGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTc  <  1:717012/140‑1 (MQ=255)
                                                                                                                         aCGAGATAAAAAAAGCCAAACGGTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTc  >  2:232945/1‑140 (MQ=255)
                                                                                                                            |                                                                                                                                        
ATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTT‑AGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTC  >  NC_000913/3005916‑3006175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A