Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F73 I1 R1
|
26 |
44.8 |
1518598 |
99.2% |
1506449 |
138.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,200,238 |
C→A |
W243L (TGG→TTG) |
ygiF ← |
inorganic triphosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,200,238 | 0 | C | A | 100.0%
| 90.6
/ NA
| 27 | W243L (TGG→TTG) | ygiF | inorganic triphosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (13/14); total (13/14) |
TCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATT > NC_000913/3200103‑3200362
|
tCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAg < 2:623472/140‑1 (MQ=255)
tCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAg < 1:460903/140‑1 (MQ=255)
tCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAg < 2:205029/140‑1 (MQ=255)
tCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAg < 1:738748/140‑1 (MQ=255)
gCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGtt > 2:296864/1‑140 (MQ=255)
gCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGtt < 2:161096/140‑1 (MQ=255)
tGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGttcttc < 1:296864/140‑1 (MQ=255)
agcagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATa > 2:629244/1‑140 (MQ=255)
aaGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTgcgc > 2:452852/1‑140 (MQ=255)
tgCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGcc > 1:170470/1‑140 (MQ=255)
tCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGc > 1:37903/1‑140 (MQ=255)
aaTACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCa > 2:570980/1‑140 (MQ=255)
ccGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTg > 2:676975/1‑140 (MQ=255)
cGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGc < 1:452852/140‑1 (MQ=255)
tGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAtg > 1:602254/1‑140 (MQ=255)
gCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAtggtgg > 1:51583/1‑139 (MQ=255)
gCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAtggtgg < 2:51583/139‑1 (MQ=255)
ccAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTc > 1:616356/1‑140 (MQ=255)
ttCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGAtt > 1:584835/1‑135 (MQ=255)
ttCGCCGCATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGAtt < 2:584835/135‑1 (MQ=255)
cgccgcATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAcgcg < 2:170470/140‑1 (MQ=255)
cATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGa < 1:570980/140‑1 (MQ=255)
cATCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGa < 2:463732/140‑1 (MQ=255)
aTCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGAt < 1:489776/140‑1 (MQ=255)
tCGTTGCCGCGTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCGGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGAtt > 1:179512/1‑140 (MQ=255)
cgTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAcgcg < 2:36435/124‑1 (MQ=255)
cgTACCAACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAcgcg > 1:36435/1‑124 (MQ=255)
|
TCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATT > NC_000913/3200103‑3200362
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
