Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R1 146 75.3 2562504 98.6% 2526628 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 632,017 (G)7→8 intergenic (+18/‑165) cstA → / → ybdD carbon starvation protein A/conserved protein YbdD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913632,0101.G96.7% 221.5 / 0.0 61intergenic (+11/‑172)cstA/ybdDcarbon starvation protein A/conserved protein YbdD
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (27/32);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

GCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCAT  >  NC_000913/631871‑632143
                                                                                                                                            |                                                                                                                                      
gCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGa                                                                                                                                         >  2:1181392/1‑140 (MQ=255)
 cACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑g                                                                                                                                       <  2:803646/140‑1 (MQ=255)
 cACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑g                                                                                                                                       <  2:593166/140‑1 (MQ=255)
     gTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑ggg‑gg                                                                                                                                  >  2:271606/1‑140 (MQ=255)
     gTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑ggg‑gg                                                                                                                                  >  1:990298/1‑140 (MQ=255)
      ttCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGa                                                                                                                                         <  1:1122784/134‑1 (MQ=255)
      ttCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGa                                                                                                                                         >  2:1122784/1‑134 (MQ=255)
      ttCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑ggg‑ggg                                                                                                                                 >  2:914122/1‑140 (MQ=255)
      ttCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑ggg‑ggg                                                                                                                                 <  1:846974/140‑1 (MQ=255)
      ttCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑ggg‑ggg                                                                                                                                 >  1:728295/1‑140 (MQ=255)
                aGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGc                                                                                                                        >  2:1001493/1‑140 (MQ=255)
                aGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGc                                                                                                                        >  1:170680/1‑140 (MQ=255)
                       aCTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGtt                                                                                                                 >  1:897394/1‑140 (MQ=255)
                       aCTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGtt                                                                                                                 >  2:1214149/1‑140 (MQ=255)
                       aCTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGtt                                                                                                                 >  2:313204/1‑140 (MQ=255)
                             ggCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTa                                                                                                           <  2:455150/140‑1 (MQ=255)
                             ggCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTa                                                                                                           <  2:987427/140‑1 (MQ=255)
                             ggCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTa                                                                                                           <  1:498369/140‑1 (MQ=255)
                             ggCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTa                                                                                                           <  2:170680/140‑1 (MQ=255)
                                aTTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTg                                                                                                        >  1:1142587/1‑140 (MQ=255)
                                   gAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGccc                                                                                                     <  1:1001493/140‑1 (MQ=255)
                                   gAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGccc                                                                                                     <  1:48881/140‑1 (MQ=255)
                                         tCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGtt                                                                                               <  2:990298/140‑1 (MQ=255)
                                         tCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGtt                                                                                               <  1:585645/140‑1 (MQ=255)
                                           cGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGtt                                                                                               >  2:224079/1‑138 (MQ=255)
                                           cGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGtt                                                                                               <  1:224079/138‑1 (MQ=255)
                                           cGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTcc                                                                                             >  1:1167790/1‑140 (MQ=255)
                                               gCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctct                                                                                         <  2:728295/140‑1 (MQ=255)
                                               gCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctct                                                                                         <  1:1139050/140‑1 (MQ=255)
                                                   aCGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAcc                                                                                     >  1:45726/1‑140 (MQ=255)
                                                   aCGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAcc                                                                                     <  1:1041655/140‑1 (MQ=255)
                                                   aCGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGTGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAcc                                                                                     >  1:766974/1‑140 (MQ=255)
                                                    cGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAccc                                                                                    >  1:348755/1‑140 (MQ=255)
                                                      gCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTa                                                                                  <  1:229559/140‑1 (MQ=255)
                                                                      aTGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgg                                                                  <  1:914122/140‑1 (MQ=255)
                                                                        gAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgggg                                                                <  1:1214149/140‑1 (MQ=255)
                                                                          aCCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCg                                                              <  2:45726/140‑1 (MQ=255)
                                                                          aCCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCg                                                              <  1:1102171/140‑1 (MQ=255)
                                                                          aCCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCg                                                              <  1:313204/140‑1 (MQ=255)
                                                                          aCCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCg                                                              <  1:1181392/140‑1 (MQ=255)
                                                                          aCCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGTGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCg                                                              <  2:766974/140‑1 (MQ=255)
                                                                            cAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAg                                                            <  2:1232174/140‑1 (MQ=255)
                                                                               tGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGa                                                             >  2:464423/1‑136 (MQ=255)
                                                                               tGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGa                                                             <  1:464423/136‑1 (MQ=255)
                                                                               tGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAgggg                                                         >  1:732881/1‑140 (MQ=255)
                                                                                      aaaTGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGAcc                                                  >  1:526815/1‑140 (MQ=255)
                                                                                             gaggagATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGcgcg                                           <  2:112974/140‑1 (MQ=255)
                                                                                                  gaTCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAt                                      <  2:732881/140‑1 (MQ=255)
                                                                                                   aTCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATa                                     >  1:703420/1‑140 (MQ=255)
                                                                                                   aTCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATa                                     >  2:1079710/1‑140 (MQ=255)
                                                                                                     cGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGc                                   >  1:1080526/1‑140 (MQ=255)
                                                                                                         gcgcAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAttt                               <  1:271606/140‑1 (MQ=255)
                                                                                                             aGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAg                           <  2:1167790/140‑1 (MQ=255)
                                                                                                             aGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAg                           >  2:286879/1‑140 (MQ=255)
                                                                                                              ggCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAt                                      <  2:857061/128‑1 (MQ=255)
                                                                                                              ggCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAt                                      >  1:857061/1‑128 (MQ=255)
                                                                                                               gCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGc                         <  2:1080526/140‑1 (MQ=255)
                                                                                                               gCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGc                         >  1:532387/1‑140 (MQ=255)
                                                                                                                    aGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGAt                    <  1:1021871/140‑1 (MQ=255)
                                                                                                                    aGGCGCACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGAt                    <  2:823793/140‑1 (MQ=255)
                                                                                                                       cgcACACTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACTCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAg                 <  2:1142587/140‑1 (MQ=255)
                                                                                                                         cacaCTAAAGTCAGAGTGAGGGG‑GGGGCTGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGCGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGc               >  2:1169980/1‑140 (MQ=255)
                                                                                                                         cacaCTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGc               >  1:79192/1‑140 (MQ=255)
                                                                                                                         cacaCTAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGc               >  2:1000934/1‑140 (MQ=255)
                                                                                                                              tAAAGTCAGAGTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCa          >  2:1239380/1‑140 (MQ=255)
                                                                                                                                      gagTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAgg                >  2:717623/1‑126 (MQ=255)
                                                                                                                                      gagTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAgg                <  1:717623/126‑1 (MQ=255)
                                                                                                                                      gagTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTc           <  2:860743/131‑1 (MQ=255)
                                                                                                                                      gagTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTc           >  1:860743/1‑131 (MQ=255)
                                                                                                                                      gagTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCAt  >  1:198527/1‑140 (MQ=255)
                                                                                                                                      gagTGAGGGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCAt  >  1:924532/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                      
GCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGG‑GGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCAT  >  NC_000913/631871‑632143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A