Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	146	75.3	2562504	98.6%	2526628	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	634,857	C→T	G174S (GGC→AGC)	ybdM ←	ParB‑like nuclease domain‑containing protein YbdM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	634,857	0	C	T	100.0%	176.1 / NA	49	G174S (GGC→AGC)	ybdM	ParB‑like nuclease domain‑containing protein YbdM
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/23); total (26/23)

AGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACA  >  NC_000913/634722‑634992

agcTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAAtt                                                                                                                                     >  1:839819/1‑140 (MQ=255)

agcTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAAtt                                                                                                                                     <  1:1261491/140‑1 (MQ=255)

            gcgcCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGtcc                                                                                                                         >  1:461990/1‑140 (MQ=255)

             cgcCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGtccc                                                                                                                        >  2:1063875/1‑140 (MQ=255)

                    tttAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCaa                                                                                                                 <  2:452421/140‑1 (MQ=255)

                        gCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTATTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTgg                                                                                                             <  2:955329/140‑1 (MQ=255)

                          tATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCt                                                                                                           <  1:114212/140‑1 (MQ=255)

                                    tCCAGGCGCGGGAATATTGTCGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACgg                                                                                                 >  2:1117684/1‑140 (MQ=255)

                                    tCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACgg                                                                                                 >  2:473295/1‑140 (MQ=255)

                                     ccAGGCGCGGGAATATTGTCGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACAGa                                                                                                <  1:1117684/140‑1 (MQ=255)

                                     ccAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGa                                                                                                <  2:839819/140‑1 (MQ=255)

                                      cAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGAc                                                                                               >  2:84844/1‑140 (MQ=255)

                                              ggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCg                                                                                       >  2:895156/1‑140 (MQ=255)

                                              ggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCg                                                                                       >  2:878178/1‑140 (MQ=255)

                                              ggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCg                                                                                       >  2:640700/1‑140 (MQ=255)

                                                aaTATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGAc                                                                                     <  1:276928/140‑1 (MQ=255)

                                                       aCGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCgg                                                                              <  1:1170428/140‑1 (MQ=255)

                                                           tCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGAt                                                                          >  2:131460/1‑140 (MQ=255)

                                                           tCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGAt                                                                          >  1:1022205/1‑140 (MQ=255)

                                                              gCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTg                                                                       <  1:84844/140‑1 (MQ=255)

                                                                        ctTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCa                                                             <  2:461990/140‑1 (MQ=255)

                                                                                ccGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGcgcg                                                     >  2:1035872/1‑140 (MQ=255)

                                                                                   ttAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTTATGGCGTCCACGAGCGCGAtt                                                  >  2:828018/1‑140 (MQ=255)

                                                                                           cTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAgc                                                        <  1:1117249/126‑1 (MQ=255)

                                                                                           cTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAgc                                                        >  2:1117249/1‑126 (MQ=255)

                                                                                              cAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGGCCACGAGCGCGATTATGACGAATaa                                       >  2:251506/1‑139 (MQ=255)

                                                                                                                gCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGAt                     <  1:878178/140‑1 (MQ=255)

                                                                                                                 cTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGAtt                    >  2:980641/1‑140 (MQ=255)

                                                                                                                    tCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTAcg                 >  2:169258/1‑140 (MQ=255)

                                                                                                                    tCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTAcg                 >  2:411202/1‑140 (MQ=255)

                                                                                                                       aTTCCCAGCTCTTTGCTAATTTTATTGTCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:895156/140‑1 (MQ=255)

                                                                                                                       aTTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:131460/140‑1 (MQ=255)

                                                                                                                       aTTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:169258/140‑1 (MQ=255)

                                                                                                                       aTTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:473295/140‑1 (MQ=255)

                                                                                                                       aTTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:640700/140‑1 (MQ=255)

                                                                                                                       aTTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:827884/140‑1 (MQ=255)

                                                                                                                         tCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTTATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGGTGCGTTGATTACGCGTAc            >  2:865191/1‑140 (MQ=255)

                                                                                                                         tCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTTAAGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGGTGGTTAGGCGTAc            >  2:284422/1‑140 (MQ=255)

                                                                                                                         tCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              >  1:807672/1‑138 (MQ=255)

                                                                                                                         tCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  2:807672/138‑1 (MQ=255)

                                                                                                                              gCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTAc                  <  2:544227/129‑1 (MQ=255)

                                                                                                                              gCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTAc                  >  1:544227/1‑129 (MQ=255)

                                                                                                                              gCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTcc       >  1:689981/1‑140 (MQ=255)

                                                                                                                              gCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTcc       >  1:358671/1‑140 (MQ=255)

                                                                                                                               ctctTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCATCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCa      <  2:1022205/140‑1 (MQ=255)

                                                                                                                                   ttGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:881667/128‑1 (MQ=255)

                                                                                                                                   ttGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              >  2:881667/1‑128 (MQ=255)

                                                                                                                                   ttGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACa  >  1:1038087/1‑140 (MQ=255)

                                                                                                                                    tGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  2:736210/127‑1 (MQ=255)

AGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACA  >  NC_000913/634722‑634992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A