Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	146	75.3	2562504	98.6%	2526628	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,824,303	T→C	Q15R (CAG→CGG)	pncC ←	NMN aminohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,824,303	0	T	C	100.0%	215.0 / NA	57	Q15R (CAG→CGG)	pncC	NMN aminohydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/31); total (26/31)

TGGCTTCGTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACG  >  NC_000913/2824166‑2824428

tGGCTTCGTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGc                                                                                                                             >  1:1261837/1‑140 (MQ=255)

  gCTTCGTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCgcccgccc                                                                                                                           <  1:748906/140‑1 (MQ=255)

   cTTCGTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCa                                                                                                                          <  1:395558/140‑1 (MQ=255)

    ttCGTTACTGTAGGTGACAAATCCGCGTTC‑AACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAAc                                                                                                                        <  1:764640/140‑1 (MQ=255)

     tCGTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAAc                                                                                                                        <  1:529485/140‑1 (MQ=255)

       gTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCt                                                                                                                      <  1:848342/140‑1 (MQ=255)

        ttACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCt                                                                                                                      >  1:80180/1‑139 (MQ=255)

        ttACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCt                                                                                                                      <  2:80180/139‑1 (MQ=255)

                ggTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTa                                                                                                             <  1:992355/140‑1 (MQ=255)

                ggTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTa                                                                                                             >  2:77962/1‑140 (MQ=255)

                ggTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTa                                                                                                             >  1:1094076/1‑140 (MQ=255)

                 gTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTaa                                                                                                            <  2:472179/140‑1 (MQ=255)

                  tGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAAc                                                                                                           <  1:53105/140‑1 (MQ=255)

                  tGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAAc                                                                                                           <  2:1094076/140‑1 (MQ=255)

                   gACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACt                                                                                                          >  1:267981/1‑140 (MQ=255)

                    aCAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTg                                                                                                         <  2:954621/140‑1 (MQ=255)

                     cAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTCAACTGc                                                                                                        >  1:966255/1‑140 (MQ=255)

                      aaaTCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGtt                                                                                                                   >  1:151055/1‑128 (MQ=255)

                      aaaTCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGtt                                                                                                                   <  2:151055/128‑1 (MQ=255)

                       aaTCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCAt                                                                                                      <  2:267981/140‑1 (MQ=255)

                               ttCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCAc                                                                                              <  2:594492/140‑1 (MQ=255)

                                  aaaCCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTCAACTGCATCAGTTCACTgt                                                                                           <  2:966255/140‑1 (MQ=255)

                                    aCCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtca                                                                                         <  1:17696/140‑1 (MQ=255)

                                     ccAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtcag                                                                                        <  2:315611/140‑1 (MQ=255)

                                      cAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtcagt                                                                                       <  2:871935/140‑1 (MQ=255)

                                        ggCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtcagtca                                                                                     >  1:957277/1‑140 (MQ=255)

                                         gCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcat                                                                                    <  2:1232746/140‑1 (MQ=255)

                                         gCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcat                                                                                    >  1:1048046/1‑140 (MQ=255)

                                           ggAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCTCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcatca                                                                                  <  2:875566/140‑1 (MQ=255)

                                           ggAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcatca                                                                                  <  2:1048046/140‑1 (MQ=255)

                                           ggAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcatca                                                                                  <  2:857199/140‑1 (MQ=255)

                                           ggAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcatca                                                                                  <  2:957277/140‑1 (MQ=255)

                                                  ccGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACctc                                                                           >  1:1189805/1‑140 (MQ=255)

                                                     gCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTgt                                                                        <  1:310378/140‑1 (MQ=255)

                                                                 aTCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAAtt                                                            >  2:610232/1‑140 (MQ=255)

                                                                  tCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAAttt                                                           >  1:610744/1‑140 (MQ=255)

                                                                   cACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTa                                                          <  1:915195/140‑1 (MQ=255)

                                                                     cTTTCGCTACCCAACCACCTGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATg                                                        <  1:1074053/140‑1 (MQ=255)

                                                                         cGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCgg                                                    >  1:1058731/1‑140 (MQ=255)

                                                                          gCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGa                                                   <  2:942992/140‑1 (MQ=255)

                                                                            tACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACa                                                 >  2:1206163/1‑140 (MQ=255)

                                                                                 aaCCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATa                                            <  2:1189805/140‑1 (MQ=255)

                                                                                   ccaccGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGc                                          >  1:131644/1‑140 (MQ=255)

                                                                                       cGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACtt                                      <  2:131644/140‑1 (MQ=255)

                                                                                        ggTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACttt                                     >  1:879968/1‑140 (MQ=255)

                                                                                              aGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAg                               <  1:555302/140‑1 (MQ=255)

                                                                                                 cTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGaa                            >  1:1252225/1‑140 (MQ=255)

                                                                                                 cTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGaa                            <  2:1261837/140‑1 (MQ=255)

                                                                                                    ggCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGAt                         >  1:1014899/1‑140 (MQ=255)

                                                                                                    ggCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGAt                         >  2:96471/1‑140 (MQ=255)

                                                                                                    ggCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGAt                         >  2:1092637/1‑140 (MQ=255)

                                                                                                    ggCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGAt                         >  2:616081/1‑140 (MQ=255)

                                                                                                         ttgttACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGgtgt                    >  2:244293/1‑140 (MQ=255)

                                                                                                                 gTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTg            <  2:603135/140‑1 (MQ=255)

                                                                                                                   tGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCa          >  2:705673/1‑140 (MQ=255)

                                                                                                                     cgcCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAAt        >  1:299285/1‑140 (MQ=255)

                                                                                                                          cGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTAc   >  2:1194472/1‑140 (MQ=255)

                                                                                                                           gggCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACg  >  2:812867/1‑140 (MQ=255)

TGGCTTCGTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACG  >  NC_000913/2824166‑2824428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A