Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R1 146 75.3 2562504 98.6% 2526628 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,102,340 (C)6→7 coding (513/720 nt) trmI ← tRNA m(7)G46 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,102,3341.C98.8% 298.9 / ‑4.4 82G173G (GGC→GGG) trmItRNA m(7)G46 methyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base C (42/39);  minor base T (1/0);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-01

CAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCA  >  NC_000913/3102195‑3102467
                                                                                                                                            |                                                                                                                                      
cAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACg                                                                                                                                         >  1:939783/1‑140 (MQ=255)
cAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACg                                                                                                                                         >  1:845200/1‑140 (MQ=255)
      tCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑‑cccccc                                                                                                                                 >  1:768095/1‑140 (MQ=255)
         tCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑‑cccccccag                                                                                                                              <  2:939783/140‑4 (MQ=255)
             cGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGc                                                                                                                           >  2:839964/1‑140 (MQ=255)
                aCGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGt                                                                                                                        <  2:684861/140‑1 (MQ=255)
                 cGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGtt                                                                                                                       <  1:1263347/140‑1 (MQ=255)
                     aTGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCt                                                                                                                   >  1:723500/1‑140 (MQ=255)
                          ggACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTa                                                                                                              >  2:689930/1‑140 (MQ=255)
                           gACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTAc                                                                                                             >  1:1133784/1‑140 (MQ=255)
                               cgGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGt                                                                                                         <  2:180070/140‑1 (MQ=255)
                                          cATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACgg                                                                                              <  1:966246/140‑1 (MQ=255)
                                            ttGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCa                                                                                            >  2:544464/1‑140 (MQ=255)
                                              gCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAcc                                                                                          >  2:583667/1‑140 (MQ=255)
                                                 ctctGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGa                                                                                       >  1:429964/1‑140 (MQ=255)
                                                  tctGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGaa                                                                                      >  1:851654/1‑140 (MQ=255)
                                                      aCAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGAt                                                                                  <  2:514410/140‑1 (MQ=255)
                                                       cAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATa                                                                                 <  2:429964/140‑1 (MQ=255)
                                                       cAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATa                                                                                 <  1:689930/140‑1 (MQ=255)
                                                             tttATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGt                                                                           <  1:544464/140‑1 (MQ=255)
                                                              ttATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGtt                                                                          >  1:930628/1‑140 (MQ=255)
                                                                    ccGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGtt                                                                          >  2:572795/1‑134 (MQ=255)
                                                                    ccGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGtt                                                                          <  1:572795/134‑1 (MQ=255)
                                                                    ccGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTtattat                                                                    >  2:602518/1‑140 (MQ=255)
                                                                         aaTAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGgcgc                                                               >  2:79855/1‑140 (MQ=255)
                                                                             gaagaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCCGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCGGTTCGGCAAACGGTACCTGAACGATACGGCGTTTATTATGGCGCGCtt                                                           <  2:1133784/140‑1 (MQ=255)
                                                                             gaagaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCtt                                                           <  1:1041469/140‑1 (MQ=255)
                                                                               agaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTtg                                                         <  2:768095/140‑1 (MQ=255)
                                                                                gaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTtgt                                                        >  1:102476/1‑140 (MQ=255)
                                                                                  cATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGc                                                      >  1:200555/1‑140 (MQ=255)
                                                                                   aTCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGcc                                                     >  2:963826/1‑140 (MQ=255)
                                                                                    tCACTTCAAGCATATGTTCCGCGTAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                    <  2:849987/140‑1 (MQ=255)
                                                                                    tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                    <  2:1041837/140‑1 (MQ=255)
                                                                                     cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGgcg                                                                <  2:444487/127‑1 (MQ=255)
                                                                                     cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGgcg                                                                >  1:444487/1‑127 (MQ=255)
                                                                                     cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGgc                                                                 >  2:783943/1‑126 (MQ=255)
                                                                                     cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGgc                                                                 <  1:783943/126‑1 (MQ=255)
                                                                                     cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCAc                                                   >  2:438489/1‑140 (MQ=255)
                                                                                     cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCAc                                                   >  2:890350/1‑140 (MQ=255)
                                                                                     cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCAc                                                   <  2:82123/140‑1 (MQ=255)
                                                                                     cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCAc                                                   >  1:865923/1‑140 (MQ=255)
                                                                                     cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCAc                                                   >  1:757789/1‑140 (MQ=255)
                                                                                        ttCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACggg                                                >  2:806159/1‑140 (MQ=255)
                                                                                        ttCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACggg                                                >  1:650059/1‑140 (MQ=255)
                                                                                         tCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGt                                               <  2:845200/140‑1 (MQ=255)
                                                                                          cAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTc                                              >  1:1009736/1‑140 (MQ=255)
                                                                                             gCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAgg                                           >  2:529737/1‑140 (MQ=255)
                                                                                               atatGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGa                                         >  1:250058/1‑140 (MQ=255)
                                                                                                 atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                       <  1:908396/140‑1 (MQ=255)
                                                                                                 atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                       <  2:865923/140‑1 (MQ=255)
                                                                                                 atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                       <  2:723500/140‑1 (MQ=255)
                                                                                                 atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                       <  1:327200/140‑1 (MQ=255)
                                                                                                 atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                       <  1:438489/140‑1 (MQ=255)
                                                                                                 atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                       <  1:397614/140‑1 (MQ=255)
                                                                                                 atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                       <  2:1009736/140‑1 (MQ=255)
                                                                                                  tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATATGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaaa                                      >  1:552175/1‑140 (MQ=255)
                                                                                                  tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaaa                                      >  2:803709/1‑140 (MQ=255)
                                                                                                  tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                       >  1:394694/1‑139 (MQ=255)
                                                                                                  tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                       <  2:394694/139‑1 (MQ=255)
                                                                                                  tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                       >  2:1228437/1‑139 (MQ=255)
                                                                                                  tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                       <  1:1228437/139‑1 (MQ=255)
                                                                                                  tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGC‑AACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaaaa                                     >  1:1180550/1‑140 (MQ=255)
                                                                                                       cGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGc                                 >  1:188353/1‑140 (MQ=255)
                                                                                                         cATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTg                               >  1:638584/1‑140 (MQ=255)
                                                                                                         cATAAGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTg                               >  2:290226/1‑140 (MQ=255)
                                                                                                          aTAAGGTTCCCAGTCGGTCGCCATATGGAATACGT‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGc                              >  2:681584/1‑140 (MQ=255)
                                                                                                            aaGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCAc                            <  2:227245/140‑1 (MQ=255)
                                                                                                             aGGTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCAcc                           <  1:79855/140‑1 (MQ=255)
                                                                                                               gTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCAt                         <  2:1089859/140‑1 (MQ=255)
                                                                                                               gTTCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCAt                         <  1:890350/140‑1 (MQ=255)
                                                                                                                 tCCCAGTCGGTCGCCATATGGAATACGCCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATg                        >  1:1102003/1‑140 (MQ=255)
                                                                                                                 tCCCAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATgc                       >  2:51867/1‑140 (MQ=255)
                                                                                                                  cccAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATgcg                      >  2:1116511/1‑140 (MQ=255)
                                                                                                                    cAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCa                    <  1:963826/140‑1 (MQ=255)
                                                                                                                    cAGTCGGTCGCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCa                    <  2:930628/140‑1 (MQ=255)
                                                                                                                        cggtcgCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGa                <  1:1116511/140‑1 (MQ=255)
                                                                                                                         ggtcgCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCt                                >  2:452934/1‑123 (MQ=255)
                                                                                                                         ggtcgCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCt                                <  1:452934/123‑1 (MQ=255)
                                                                                                                         ggtcgCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGaa               >  1:752831/1‑140 (MQ=255)
                                                                                                                          gtcgCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGTTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAAt              >  1:1090760/1‑140 (MQ=255)
                                                                                                                          gtcgCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAAt              >  1:577411/1‑140 (MQ=255)
                                                                                                                          gtcgCCATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAAt              >  1:174997/1‑140 (MQ=255)
                                                                                                                              ccATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTc          <  1:583667/140‑1 (MQ=255)
                                                                                                                              ccATATGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTc          <  2:650059/140‑1 (MQ=255)
                                                                                                                                   tGGAATACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGaa     <  1:602518/140‑1 (MQ=255)
                                                                                                                                      aaTACGC‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCa  <  1:290226/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                      
CAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCA  >  NC_000913/3102195‑3102467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A