Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	128	54.7	1845674	99.3%	1832754	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,102,340	(C)_6→7	coding (513/720 nt)	trmI ←	tRNA m(7)G46 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,102,334	1	.	C	100.0%	171.7 / NA	48	G173G (GGC→GGG)	trmI	tRNA m(7)G46 methyltransferase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (21/27); total (21/27)

TTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAA  >  NC_000913/3102199‑3102464

tttCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑cccc                                                                                                                                <  2:71347/140‑1 (MQ=255)

tttCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑cccc                                                                                                                                <  2:561114/140‑1 (MQ=255)

                       gACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTAc                                                                                                          <  2:221056/140‑1 (MQ=255)

                                      cATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACgg                                                                                           <  2:221187/140‑1 (MQ=255)

                                      cATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACgg                                                                                           <  2:419088/140‑1 (MQ=255)

                                        ttcctctctGACAGGTTTTAATACCCGTCAATAGAAGAAATCACTCCAGGCATAGGTTCCGCTTAAGGTCCCCGGTCGGTGGCCTTATGAAATCGG‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAc                                                                                        <  2:434026/137‑1 (MQ=255)

                                        ttGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCa                                                                                         >  2:95283/1‑140 (MQ=255)

                                        ttGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCa                                                                                         >  2:613785/1‑140 (MQ=255)

                                        ttGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCa                                                                                         >  1:42502/1‑140 (MQ=255)

                                         tGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAc                                                                                        <  2:569019/140‑1 (MQ=255)

                                          gCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAcc                                                                                       >  1:410569/1‑140 (MQ=255)

                                          gCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAcc                                                                                       >  1:637642/1‑140 (MQ=255)

                                          gCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAcc                                                                                       >  1:264035/1‑140 (MQ=255)

                                          gCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAcc                                                                                       >  2:771411/1‑140 (MQ=255)

                                              tctGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGaa                                                                                   <  2:632452/140‑1 (MQ=255)

                                               ctGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAAc                                                                                  >  1:451396/1‑140 (MQ=255)

                                                tGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACgg                                                                                           <  2:188774/130‑1 (MQ=255)

                                                tGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACgg                                                                                           >  1:188774/1‑130 (MQ=255)

                                                     ggTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACg                                                                            <  1:515161/140‑1 (MQ=255)

                                                         tttATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGt                                                                        <  2:431823/140‑1 (MQ=255)

                                                            ataACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTtta                                                                     <  1:909848/140‑1 (MQ=255)

                                                                ccGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTtattat                                                                 >  1:368771/1‑140 (MQ=255)

                                                                 cGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATg                                                                >  1:242308/1‑140 (MQ=255)

                                                                        agaagaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTCTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCt                                                         <  2:637642/140‑1 (MQ=255)

                                                                          aagaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCttt                                                       >  2:50685/1‑140 (MQ=255)

                                                                                tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGTAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 <  1:50685/140‑1 (MQ=255)

                                                                                tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 >  1:211807/1‑140 (MQ=255)

                                                                                tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 <  1:226135/140‑1 (MQ=255)

                                                                                tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 <  2:439301/140‑1 (MQ=255)

                                                                                tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 <  1:440988/140‑1 (MQ=255)

                                                                                tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 <  1:92082/140‑1 (MQ=255)

                                                                                tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 <  1:642074/140‑1 (MQ=255)

                                                                                 cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCAc                                                >  2:783747/1‑140 (MQ=255)

                                                                                    ttCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACggg                                             >  2:367663/1‑140 (MQ=255)

                                                                                        aGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAg                                         <  1:619216/140‑1 (MQ=255)

                                                                                         gCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAgg                                        >  1:320330/1‑140 (MQ=255)

                                                                                             atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                    <  1:783747/140‑1 (MQ=255)

                                                                                             atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                    <  1:453298/140‑1 (MQ=255)

                                                                                             atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                    <  2:211807/140‑1 (MQ=255)

                                                                                             atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                    <  1:95283/140‑1 (MQ=255)

                                                                                              tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaaa                                   >  2:216533/1‑140 (MQ=255)

                                                                                                   cGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGc                              >  1:714323/1‑140 (MQ=255)

                                                                                                   cGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGc                              >  2:112122/1‑140 (MQ=255)

                                                                                                      aTAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGc                           <  1:216533/140‑1 (MQ=255)

                                                                                                         aGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCAcc                        <  2:264035/140‑1 (MQ=255)

                                                                                                              cccAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATgcg                   <  1:273019/140‑1 (MQ=255)

                                                                                                                     ggtcgCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGaa            >  1:796770/1‑140 (MQ=255)

                                                                                                                      gtcgCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAAt               >  2:379098/1‑136 (MQ=255)

                                                                                                                      gtcgCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAAt               <  1:379098/136‑1 (MQ=255)

                                                                                                                         gCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGt        <  1:771411/140‑1 (MQ=255)

                                                                                                                               tGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGaa  <  1:613785/140‑1 (MQ=255)

TTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAA  >  NC_000913/3102199‑3102464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A