Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	146	75.3	2562504	98.6%	2526628	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,178,848	T→C	L245P (CTG→CCG)	tolC →	outer membrane channel TolC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,178,848	0	T	C	100.0%	308.2 / NA	82	L245P (CTG→CCG)	tolC	outer membrane channel TolC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/48); total (34/48)

CGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGCGGTTCGAAAACCCGTGGT  >  NC_000913/3178711‑3178984

cgcgGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGt                                                                                                                                          >  1:1086819/1‑140 (MQ=255)

  cgGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTa                                                                                                                                        <  2:207124/140‑1 (MQ=255)

   ggTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTAc                                                                                                                                       <  1:883840/140‑1 (MQ=255)

   ggTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTAc                                                                                                                                       <  1:797019/140‑1 (MQ=255)

   ggTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTAc                                                                                                                                       >  2:630733/1‑140 (MQ=255)

     tAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAg                                                                                                                                     >  1:150708/1‑140 (MQ=255)

            gcTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCt                                                                                                                              <  1:205712/140‑1 (MQ=255)

            gcTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCt                                                                                                                              <  1:673676/140‑1 (MQ=255)

             cTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGc                                                                                                                               <  2:253475/138‑1 (MQ=255)

             cTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGc                                                                                                                               >  1:253475/1‑138 (MQ=255)

               gcgcCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGGCAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGa                                                                                                                           >  2:966847/1‑140 (MQ=255)

               gcgcCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGa                                                                                                                           >  1:519307/1‑140 (MQ=255)

                  ccAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGcc                                                                                                                        <  1:984335/140‑1 (MQ=255)

                  ccAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGcc                                                                                                                        <  1:763207/140‑1 (MQ=255)

                      aTCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGa                                                                                                                    >  2:1244418/1‑140 (MQ=255)

                             gTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCtt                                                                                                                             >  2:294803/1‑124 (MQ=255)

                             gTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCtt                                                                                                                             <  1:294803/124‑1 (MQ=255)

                               aaCTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGgcgcg                                                                                                           <  2:281451/140‑1 (MQ=255)

                                        ccGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAAt                                                                                                  >  1:395355/1‑140 (MQ=255)

                                        ccGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAAt                                                                                                  <  1:667909/140‑1 (MQ=255)

                                        ccGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAAt                                                                                                  >  2:371290/1‑140 (MQ=255)

                                        ccGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAAt                                                                                                  >  1:254892/1‑140 (MQ=255)

                                            aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  2:21464/1‑140 (MQ=255)

                                            aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  1:614866/1‑140 (MQ=255)

                                            aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              <  2:614866/140‑1 (MQ=255)

                                            aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  1:493187/1‑140 (MQ=255)

                                            aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  2:646663/1‑140 (MQ=255)

                                            aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  2:1250713/1‑140 (MQ=255)

                                            aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  2:845447/1‑140 (MQ=255)

                                            aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              <  1:193264/140‑1 (MQ=255)

                                            aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  1:1192701/1‑140 (MQ=255)

                                            aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  2:923688/1‑140 (MQ=255)

                                            aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              <  1:1061531/140‑1 (MQ=255)

                                            aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              <  1:827175/140‑1 (MQ=255)

                                             aCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGcc                                                                                             <  1:89881/140‑1 (MQ=255)

                                                ggCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAgg                                                                                          <  1:1188117/140‑1 (MQ=255)

                                                 gCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgc                                                                                         >  2:927972/1‑140 (MQ=255)

                                                 gCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgc                                                                                         >  1:866866/1‑140 (MQ=255)

                                                 gCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgc                                                                                         >  1:71116/1‑140 (MQ=255)

                                                 gCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgc                                                                                         >  2:627723/1‑140 (MQ=255)

                                                 gCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgc                                                                                         >  1:1049364/1‑140 (MQ=255)

                                                  cTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgcg                                                                                        <  2:81149/140‑1 (MQ=255)

                                                   tGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgcgc                                                                                       <  2:235972/140‑1 (MQ=255)

                                                   tGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgcgc                                                                                       <  2:232737/140‑1 (MQ=255)

                                                       cTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGa                                                                                   <  2:150708/140‑1 (MQ=255)

                                                       cTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGa                                                                                   <  2:716641/140‑1 (MQ=255)

                                                       cTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGa                                                                                   <  1:927972/140‑1 (MQ=255)

                                                       cTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGa                                                                                   <  1:1199331/140‑1 (MQ=255)

                                                           aTGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGt                                                                               <  2:571434/140‑1 (MQ=255)

                                                               cGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCGCt                                                                           >  2:785141/1‑140 (MQ=255)

                                                                      tttAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGAc                                                                    >  1:952839/1‑140 (MQ=255)

                                                                      tttAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGAc                                                                    >  2:313699/1‑140 (MQ=255)

                                                                       ttAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGAct                                                                   <  2:1049364/140‑1 (MQ=255)

                                                                                aCAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTa                                                          >  2:915750/1‑140 (MQ=255)

                                                                                aCAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTa                                                          <  1:209136/140‑1 (MQ=255)

                                                                                     ccACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGc                                                     <  2:569142/140‑1 (MQ=255)

                                                                                     ccACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGc                                                     <  1:785141/140‑1 (MQ=255)

                                                                                     ccACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGc                                                     <  1:630733/140‑1 (MQ=255)

                                                                                         aGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCt                                                 >  2:345385/1‑140 (MQ=255)

                                                                                           ccGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTAc                                               <  2:687083/140‑1 (MQ=255)

                                                                                              gTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCgg                                            >  2:756217/1‑140 (MQ=255)

                                                                                               ttAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCggg                                           <  2:703053/140‑1 (MQ=255)

                                                                                                tAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGa                                          >  1:490766/1‑140 (MQ=255)

                                                                                                tAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGa                                          <  1:966847/140‑1 (MQ=255)

                                                                                                        tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  2:71116/140‑1 (MQ=255)

                                                                                                        tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  1:1244418/140‑1 (MQ=255)

                                                                                                        tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  1:1057465/140‑1 (MQ=255)

                                                                                                        tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  1:59778/140‑1 (MQ=255)

                                                                                                        tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  1:646663/140‑1 (MQ=255)

                                                                                                        tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  1:915750/140‑1 (MQ=255)

                                                                                                        tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  2:17269/140‑1 (MQ=255)

                                                                                                        tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  2:1192701/140‑1 (MQ=255)

                                                                                                         gctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGaca                                 <  1:313699/140‑1 (MQ=255)

                                                                                                         gctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGaca                                 <  2:952839/140‑1 (MQ=255)

                                                                                                                  agCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTtata                      >  1:83733/1‑140 (MQ=255)

                                                                                                                    ccGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGc                    >  1:821206/1‑140 (MQ=255)

                                                                                                                    ccGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGc                    >  2:1065433/1‑140 (MQ=255)

                                                                                                                            cGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGCGGTTCGaa            <  1:627723/140‑1 (MQ=255)

                                                                                                                                  cTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGCGGTTCGAAAACCCg      <  1:425873/140‑1 (MQ=255)

                                                                                                                                    gTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTCTTATAGCGGTTCGAAAACCCg      <  2:490766/140‑1 (MQ=255)

                                                                                                                                     tCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTCATAGCGGTTCGAAAACCCgtgg   >  1:820102/1‑140 (MQ=255)

                                                                                                                                      cGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGCGGTTCGAAAACCCgtggt  <  2:1086819/140‑1 (MQ=255)

                                                                                                                                      cGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGCGGTTCGAAAACCCg      <  1:451726/136‑1 (MQ=255)

CGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGCGGTTCGAAAACCCGTGGT  >  NC_000913/3178711‑3178984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A