Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	146	75.3	2562504	98.6%	2526628	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,277,706	(A)_7→8	coding (370/465 nt)	yhaV →	ribosome‑dependent mRNA interferase toxin YhaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,699	1	.	A	96.2%	177.0 / ‑0.3	52	coding (363/465 nt)	yhaV	ribosome‑dependent mRNA interferase toxin YhaV
Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (28/22); total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

TAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAT  >  NC_000913/3277560‑3277829

tAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGt                                                                                                                                      >  1:75127/1‑140 (MQ=255)

 aGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑a                                                                                                                                    >  2:832374/1‑140 (MQ=255)

     tctGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aa                                                                                                                               <  1:458670/140‑1 (MQ=255)

      ctGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aaa                                                                                                                              >  1:1258320/1‑140 (MQ=255)

       tGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aaaa                                                                                                                             >  1:856524/1‑140 (MQ=255)

       tGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aaaa                                                                                                                             >  1:1233746/1‑140 (MQ=255)

          gAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aaaaaca                                                                                                                          <  2:1233746/140‑4 (MQ=255)

          gAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aaaaaca                                                                                                                          <  1:919454/140‑4 (MQ=255)

          gAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aaaaaca                                                                                                                          <  1:117070/140‑4 (MQ=255)

            aaaaTAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aaaaacaga                                                                                                                        <  1:1108948/140‑6 (MQ=255)

            aaaaTAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aaaaacaga                                                                                                                        <  2:75127/140‑6 (MQ=255)

            aaaaTAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aaaaacaga                                                                                                                        <  2:430080/140‑6 (MQ=255)

             aaaTAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aaaaaacaga                                                                                                                       <  2:1036432/140‑7 (MQ=255)

                 aaaGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCt                                                                                                                    <  2:856524/140‑1 (MQ=255)

                        ggTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCt                                                                                                                    >  2:218996/1‑133 (MQ=255)

                        ggTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCt                                                                                                                    <  1:218996/133‑1 (MQ=255)

                        ggTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGt                                                                                                             >  2:371260/1‑140 (MQ=255)

                        ggTCACGGATAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGt                                                                                                             >  2:1081354/1‑140 (MQ=255)

                         gTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTa                                                                                                            >  1:318563/1‑140 (MQ=255)

                         gTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTa                                                                                                            >  2:192826/1‑140 (MQ=255)

                         gTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aaaaacaga                                                                                                                        >  2:1026031/1‑122 (MQ=255)

                         gTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑aaa‑aaaaacaga                                                                                                                        <  1:1026031/127‑6 (MQ=255)

                                gTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCa                                                                                                     <  2:153077/140‑1 (MQ=255)

                                  aaaaTTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCaaa                                                                                                   <  1:107847/140‑1 (MQ=255)

                                         ggTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTaa                                                                                            >  1:364205/1‑140 (MQ=255)

                                                gTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAgg                                                                                     >  2:271984/1‑140 (MQ=255)

                                                 tCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGa                                                                                    <  1:605561/140‑1 (MQ=255)

                                                     tATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtc                                                                                <  1:371260/140‑1 (MQ=255)

                                                          tctcTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATcctcct                                                                           >  1:273907/1‑140 (MQ=255)

                                                            tcttctTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGc                                                                         >  2:1077406/1‑140 (MQ=255)

                                                             cttcttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGcc                                                                        <  2:1232802/140‑1 (MQ=255)

                                                                    cGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                 <  2:985493/140‑1 (MQ=255)

                                                                         taGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAcc                                                            <  1:832374/140‑1 (MQ=255)

                                                                          aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAGAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAccc                                                           >  1:496680/1‑140 (MQ=255)

                                                                            tGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                         <  2:364205/140‑1 (MQ=255)

                                                                               aaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGa                                                                      >  2:1104285/1‑124 (MQ=255)

                                                                               aaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGa                                                                      <  1:1104285/124‑1 (MQ=255)

                                                                               aaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAcc                                                      <  1:1077406/140‑1 (MQ=255)

                                                                                   gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCga                                                   >  2:712040/1‑139 (MQ=255)

                                                                                   gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCga                                                   >  2:72564/1‑139 (MQ=255)

                                                                                   gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCga                                                   <  1:72564/139‑1 (MQ=255)

                                                                                   gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCga                                                   <  1:712040/139‑1 (MQ=255)

                                                                                               atTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                      <  2:765223/140‑1 (MQ=255)

                                                                                               atTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAGAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                      <  2:496680/140‑1 (MQ=255)

                                                                                                   tGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCa                                  >  1:617606/1‑140 (MQ=255)

                                                                                                   tGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCa                                  >  1:1216483/1‑140 (MQ=255)

                                                                                                          gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTACAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                           >  2:1156852/1‑140 (MQ=255)

                                                                                                          gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                           >  2:298735/1‑140 (MQ=255)

                                                                                                          gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                           >  2:175764/1‑140 (MQ=255)

                                                                                                            tgAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAgaagaa                                       <  2:226901/126‑1 (MQ=255)

                                                                                                            tgAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAgaagaa                                       >  1:226901/1‑126 (MQ=255)

                                                                                                            tgAACGATGAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGgtg                         <  2:1258320/140‑1 (MQ=255)

                                                                                                                    gAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAt                 >  2:904147/1‑140 (MQ=255)

                                                                                                                    gAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAt                 >  2:1160921/1‑140 (MQ=255)

                                                                                                                    gAAAACACTCTGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAt                 >  2:64505/1‑140 (MQ=255)

                                                                                                                             ctGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGGAGAAACCCATTGATGGTGTTCACAATGCCCGCCGa        >  2:882330/1‑140 (MQ=255)

                                                                                                                             ctGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAGCCCATTGATGgt                          <  2:122835/122‑1 (MQ=255)

                                                                                                                             ctGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGgt                          >  1:122835/1‑122 (MQ=255)

                                                                                                                             ctGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGa        >  2:120911/1‑140 (MQ=255)

                                                                                                                              tGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGaa       <  2:273907/140‑1 (MQ=255)

                                                                                                                              tGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATACAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGaa       >  2:433561/1‑140 (MQ=255)

                                                                                                                              tGCGCACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATACAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGaa       <  1:433561/140‑1 (MQ=255)

                                                                                                                               gcgcACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAc      >  1:45177/1‑140 (MQ=255)

                                                                                                                               gcgcACCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAc      >  2:1020181/1‑140 (MQ=255)

                                                                                                                                   aCCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAt  <  2:617606/140‑1 (MQ=255)

                                                                                                                                   aCCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAt  <  2:41762/140‑1 (MQ=255)

                                                                                                                                   aCCTACGGTAAAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACCGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAt  >  2:660292/1‑140 (MQ=255)

TAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAA‑AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAT  >  NC_000913/3277560‑3277829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A