Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 162 86.7 2935410 99.3% 2914862 138.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,279,346 G→A A145T (GCT→ACT)  kbaZ → tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,279,3460GA100.0% 200.2 / NA 58A145T (GCT→ACT) kbaZtagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/30);  total (28/30)

CCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGG  >  NC_000913/3279214‑3279481
                                                                                                                                    |                                                                                                                                       
ccggccgCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAAcg                                                                                                                                  <  2:473482/140‑1 (MQ=255)
     cgCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGcgccg                                                                                                                             <  2:228237/140‑1 (MQ=255)
                 ggCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGa                                                                                                                 >  2:50029/1‑140 (MQ=255)
                 ggCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGa                                                                                                                 <  1:442310/140‑1 (MQ=255)
                            atgatTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGgaa                                                                                                      >  1:889838/1‑140 (MQ=255)
                            atgatTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGgaa                                                                                                      <  1:715092/140‑1 (MQ=255)
                            atgatTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGgaa                                                                                                      >  2:532311/1‑140 (MQ=255)
                            atgatTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGgaa                                                                                                      >  1:1133359/1‑140 (MQ=255)
                            atgatTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGgaa                                                                                                      >  2:1203375/1‑140 (MQ=255)
                                  tgattAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAAcc                                                                                                >  1:976404/1‑140 (MQ=255)
                                   gattAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCt                                                                                               <  1:296585/140‑1 (MQ=255)
                                   gattAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCt                                                                                               <  1:50029/140‑1 (MQ=255)
                                   gattAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCt                                                                                               >  2:946455/1‑140 (MQ=255)
                                      tAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTc                                                                                            <  2:889838/140‑1 (MQ=255)
                                         aaGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGGGGCGGAAGAAACCTTTCttt                                                                                         >  2:305229/1‑139 (MQ=255)
                                          aGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGa                                                                                        <  2:1393340/140‑1 (MQ=255)
                                           gCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGaa                                                                                       <  2:577010/140‑1 (MQ=255)
                                              aCGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAacac                                                                                    >  2:761822/1‑140 (MQ=255)
                                              aCGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAacac                                                                                    >  2:1183069/1‑140 (MQ=255)
                                              aCGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAacac                                                                                    <  1:98851/140‑1 (MQ=255)
                                                    cGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGc                                                                              >  1:839166/1‑140 (MQ=255)
                                                    cGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGc                                                                              <  1:946455/140‑1 (MQ=255)
                                                       cAGGATTC‑AAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAg                                                                          <  1:1461523/140‑1 (MQ=255)
                                                        aGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAg                                                                          <  1:1203375/140‑1 (MQ=255)
                                                         ggATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGc                                                                         >  2:518353/1‑140 (MQ=255)
                                                         ggATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGc                                                                         <  1:173818/140‑1 (MQ=255)
                                                          gATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGcc                                                                        >  1:147938/1‑140 (MQ=255)
                                                                aaaaaaTCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTg                                                                  <  2:839166/140‑1 (MQ=255)
                                                                aaaaaaTCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTg                                                                  <  2:1133359/140‑1 (MQ=255)
                                                                  aaaaTCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGa                                                                <  2:568679/140‑1 (MQ=255)
                                                                  aaaaTCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGa                                                                <  1:518353/140‑1 (MQ=255)
                                                                   aaaTCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAg                                                               <  2:976404/140‑1 (MQ=255)
                                                                    aaTCCACCTTGATTGCAGCATCTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGt                                                              <  2:1340354/140‑1 (MQ=255)
                                                                     aTCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTa                                                             >  1:1340086/1‑140 (MQ=255)
                                                                           cTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCAt                                                       <  2:975423/140‑1 (MQ=255)
                                                                              gATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTgg                                                    >  2:136109/1‑140 (MQ=255)
                                                                                    agcaTGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTAt                                                            <  1:487157/126‑1 (MQ=255)
                                                                                    agcaTGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTAt                                                            >  2:487157/1‑126 (MQ=255)
                                                                                      caTGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAg                                            <  1:532311/140‑1 (MQ=255)
                                                                                         gTCCTGTCAGGACGATCCGATCCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGc                                         <  2:1340086/140‑1 (MQ=255)
                                                                                          tCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGa                                              <  2:1307783/134‑1 (MQ=255)
                                                                                          tCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGa                                              >  1:1307783/1‑134 (MQ=255)
                                                                                          tCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGcc                                        >  2:205032/1‑140 (MQ=255)
                                                                                           ccTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCg                                       <  2:147938/140‑1 (MQ=255)
                                                                                                    aCGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTggc                              >  1:308957/1‑140 (MQ=255)
                                                                                                    aCGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTggc                              >  1:289281/1‑140 (MQ=255)
                                                                                                    aCGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGGATTGCATTGGTACCGAAGTGCCGGTACCCggc                              >  2:211046/1‑140 (MQ=255)
                                                                                                              tCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATg                    <  1:205032/140‑1 (MQ=255)
                                                                                                                        gatgaCATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAg          <  1:136109/140‑1 (MQ=255)
                                                                                                                         atgaCATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGc         >  2:965325/1‑140 (MQ=255)
                                                                                                                          tgaCATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCg        >  2:1352402/1‑140 (MQ=255)
                                                                                                                          tgaCATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCg        >  2:449376/1‑140 (MQ=255)
                                                                                                                          tgaCATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCg        >  2:912763/1‑140 (MQ=255)
                                                                                                                            aCATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAg      <  1:211046/140‑1 (MQ=255)
                                                                                                                              aTCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCt    >  2:234430/1‑140 (MQ=255)
                                                                                                                                cGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAg      >  2:78588/1‑136 (MQ=255)
                                                                                                                                cGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAg      <  1:78588/136‑1 (MQ=255)
                                                                                                                                cGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTgg  >  2:988979/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                       
CCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGG  >  NC_000913/3279214‑3279481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A