breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 34,120 (T)9→8 intergenic (+82/‑180) carB → / → caiF carbamoyl phosphate synthetase subunit beta/DNA‑binding transcriptional activator CaiF
RA 40,458 G→A L492F (CTC→TTC)  caiT ← L‑carnitine:gamma‑butyrobetaine antiporter
RA 51,368 T→C Y80C (TAC→TGC)  apaG ← DUF525 domain‑containing protein ApaG
RA 66,776 T→C intergenic (‑226/+59) araD ← / ← araA L‑ribulose‑5‑phosphate 4‑epimerase AraD/L‑arabinose isomerase
RA 69,527 G→A A174A (GCC→GCT araB ← ribulokinase
RA 70,787 T→C F134S (TTT→TCT)  araC → DNA‑binding transcriptional dual regulator AraC
RA 88,184 G→A A53T (GCA→ACA)  cra → DNA‑binding transcriptional dual regulator Cra
RA 112,780 A→G V147A (GTA→GCA)  coaE ← dephospho‑CoA kinase
RA 132,467 A→G T285A (ACG→GCG)  acnB → hypothetical protein
RA 148,589 A→G A69A (GCT→GCC panC ← pantothenate synthetase
RA 150,822 T→C A44A (GCA→GCG yadC ← fimbrial tip‑adhesin YadC
RA 155,950 C→A S84S (TCG→TCT yadV ← putative fimbrial chaperone YadV
RA 157,460 T→C G91G (GGA→GGG folK ← 2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 272,361 A→G intergenic (+171/‑219) insN → / → eyeA CP4‑6 prophage; IS911A regulator fragment/CP4‑6 prophage; small RNA EyeA
RA 379,621 A→G R87R (CGT→CGC frmR ← DNA‑binding transcriptional repressor FrmR
RA 385,574 A→G S115G (AGC→GGC)  tauA → taurine ABC transporter periplasmic binding protein
RA 457,597 C→T H58Y (CAT→TAT)  clpX → ATP‑dependent Clp protease ATP‑binding subunit ClpX
RA 485,714 A→G intergenic (‑95/‑47) acrA ← / → acrR multidrug efflux pump membrane fusion lipoprotein AcrA/DNA‑binding transcriptional repressor AcrR
RA 495,718 A→G Y200C (TAC→TGC)  htpG → chaperone protein HtpG
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 555,234 A→G E208E (GAA→GAG cysS → cysteine‑‑tRNA ligase
RA 585,523 A→G S37S (AGT→AGC ompT ← outer membrane protease VII (outer membrane protein 3b)
RA 614,801 A→G L215L (TTA→TTG entF → apo‑serine activating enzyme
RA 695,009 T→C intergenic (‑54/‑92) miaB ← / → ubiF isopentenyl‑adenosine A37 tRNA methylthiolase/2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase
RA 697,161 C→T intergenic (‑28/+352) metT ← / ← asnB tRNA‑Met/asparagine synthetase B
RA 734,826 A→G pseudogene (607/1434 nt) ybfO → conserved protein, rhs‑like YbfO
RA 786,366 A→C H245P (CAC→CCC)  aroG → 3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive
RA 792,586 A→G S401S (AGT→AGC modF ← ABC family protein ModF
RA 838,757 A→G L151L (TTG→CTG)  ybiX ← PKHD‑type hydroxylase YbiX
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 906,746 A→G V3A (GTA→GCA)  ybjS ← putative NAD(P)H‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
RA 912,157 A→G intergenic (‑7/+5) hcr ← / ← hcp NADH oxidoreductase/protein S‑nitrosylase
RA 913,668 C→T A49A (GCG→GCA hcp ← protein S‑nitrosylase
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 935,625 A→G Q801R (CAG→CGG)  ftsK → cell division DNA translocase FtsK
RA 943,728 C→A C105* (TGC→TGA dmsB → dimethyl sulfoxide reductase subunit B
RA 955,255 A→G Y460H (TAC→CAC)  ycaO ← ribosomal protein S12 methylthiotransferase accessory factor YcaO
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,018,893 T→C S137P (TCT→CCT)  matP → macrodomain Ter protein
RA 1,027,552 T→C E74E (GAA→GAG yccT ← DUF2057 domain‑containing protein YccT
RA 1,035,301 A→G Y79C (TAC→TGC)  hyaC → hydrogenase 1 cytochrome b subunit
RA 1,112,249 (G)6→7 coding (1387/2544 nt) opgH → osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
RA 1,138,175 (G)5→6 coding (805/942 nt) flgJ → putative peptidoglycan hydrolase FlgJ
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,156,523 T→C D254D (GAT→GAC holB → DNA polymerase III subunit delta'
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,241,823 A→G S83S (AGT→AGC cvrA ← putative K(+):H(+) antiporter
RA 1,245,862 (C)6→5 coding (1515/1698 nt) treA ← periplasmic trehalase
RA 1,260,895 (A)7→8 intergenic (‑92/+33) dauA ← / ← prs aerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase
RA 1,271,969 A→G intergenic (‑120/‑150) chaA ← / → chaB Na(+)/K(+):H(+) antiporter ChaA/putative cation transport regulator ChaB
RA 1,308,088 (A)6→5 coding (558/1461 nt) clsA ← cardiolipin synthase A
RA 1,308,744 (G)5→6 intergenic (‑99/‑44) clsA ← / → yciY cardiolipin synthase A/uncharacterized protein YciY
RA 1,331,303 T→C L86L (TTG→CTG)  topA → DNA topoisomerase 1
RA 1,331,930 A→G T295A (ACC→GCC)  topA → DNA topoisomerase 1
RA 1,338,582 C→T L193L (TTG→TTA ribA ← GTP cyclohydrolase 2
RA 1,352,858 C→T A257A (GCG→GCA sapD ← putrescine ABC exporter ATP binding protein SapD
RA 1,438,254 G→A A844V (GCC→GTC)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,438,505 A→G R760R (CGT→CGC ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,442,663 G→A L61L (CTG→TTG)  ldhA ← D‑lactate dehydrogenase
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,546,319 A→G L284P (CTG→CCG)  yddG ← aromatic amino acid exporter
RA 1,591,989 T→C N63S (AAC→AGC)  hipA ← serine/threonine kinase HipA
RA 1,593,065 T→C pseudogene (5022/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,597,313 (C)7→8 pseudogene (774/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,661,994 G→A G480D (GGC→GAC)  ynfF → putative selenate reductase YnfF
RA 1,662,571 T→C G672G (GGT→GGC ynfF → putative selenate reductase YnfF
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,776,263 T→C V226A (GTG→GCG)  ydiF → putative acetate‑CoA transferase
RA 1,779,550 A→G intergenic (‑249/‑67) ydiP ← / → ydiQ putative DNA‑binding transcriptional regulator YdiP/putative electron transfer flavoprotein subunit YdiQ
RA 1,780,988 T→C R196R (CGT→CGC ydiR → putative electron transfer flavoprotein subunit YdiR
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,894,349 (A)8→9 coding (217/300 nt) yoaC → DUF1889 domain‑containing protein YoaC
RA 1,918,648 T→C V380A (GTG→GCG)  yebT → intermembrane transport protein YebT
RA 1,919,937 T→C L810L (TTG→CTG)  yebT → intermembrane transport protein YebT
RA 1,974,705 T→C D207G (GAC→GGC)  cheA ← chemotaxis protein CheA
RA 1,976,233 A→C I8S (ATT→AGT)  motB ← motility protein B
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,996,183 T→C N217S (AAC→AGC)  sdiA ← DNA‑binding transcriptional dual regulator SdiA
RA 2,051,958 C→T Q2341* (CAA→TAA)  yeeJ → inverse autotransporter adhesin
RA 2,063,707 G→A A254V (GCT→GTT)  cobT ← nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,176,729 T→G Q159P (CAG→CCG)  gatY ← tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY
RA 2,219,789 T→C N734S (AAT→AGT)  bglX ← beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,223,852 G→A P556P (CCG→CCA dld → D‑lactate dehydrogenase
RA 2,251,588 A→G D250G (GAC→GGC)  nfo → endonuclease IV
RA 2,281,869 G→A G413E (GGA→GAA)  radD → putative DNA repair helicase RadD
RA 2,282,845 (T)6→5 intergenic (+44/+95) rplY → / ← yejK 50S ribosomal subunit protein L25/nucleoid‑associated protein YejK
RA 2,320,126 G→A T28T (ACG→ACA atoS → sensory histidine kinase AtoS
RA 2,338,718 T→C T235A (ACC→GCC)  gyrA ← DNA gyrase subunit A
RA 2,356,131 T→C V204A (GTA→GCA)  glpC → anaerobic glycerol‑3‑phosphate dehydrogenase subunit C
RA 2,383,165 T→C N151N (AAT→AAC elaD → protease ElaD
RA 2,385,959 +C coding (100/972 nt) yfbL → putative peptidase YfbL
RA 2,424,211 T→C H100R (CAC→CGC)  hisP ← lysine/arginine/ornithine ABC transporter/histidine ABC transporter, ATP binding subunit
RA 2,444,682 T→C T24A (ACC→GCC)  epmC ← EF‑P‑Lys34 hydroxylase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,466,397 T→C intergenic (+14/‑148) argW → / → intS tRNA‑Arg/CPS‑53 (KpLE1) prophage; prophage CPS‑53 integrase
RA 2,494,217 T→C V62V (GTA→GTG yfdX ← protein YfdX
RA 2,507,863 A→G V11A (GTG→GCG)  fryC ← putative PTS enzyme IIC component FryC
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,547,294 A→G T208A (ACC→GCC)  murP → N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
RA 2,582,172 Δ1 bp coding (606/1044 nt) ypfG ← DUF1176 domain‑containing protein YpfG
RA 2,618,285 T→C P164P (CCA→CCG hda ← inibitor of reinitiation of DNA replication
RA 2,620,208 (A)8→7 intergenic (‑48/+38) uraA ← / ← upp hypothetical protein/uracil phosphoribosyltransferase
RA 2,630,187 A→G K132K (AAA→AAG yfgH → lipoprotein YfgH
RA 2,697,639 A→G Y73Y (TAT→TAC tadA ← tRNA adenosine(34) deaminase
RA 2,701,367 T→C K121K (AAA→AAG pdxJ ← pyridoxine 5'‑phosphate synthase
RA 2,734,543 (C)8→9 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,742,721 C→T G113G (GGC→GGT dgcN → diguanylate cyclase DgcN
RA 2,765,794 (C)7→8 intergenic (‑18/‑124) yfjM ← / → rnlA CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system
RA 2,776,382 A→G intergenic (+5/‑4) yfjX → / → yfjY CP4‑57 prophage; putative antirestriction protein/CP4‑57 prophage; RadC‑like JAB domain‑containing protein YfjY
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,905,219 (C)6→7 coding (200/672 nt) queE ← putative 7‑carboxy‑7‑deazaguanine synthase QueE
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 3,024,564 A→G T72A (ACC→GCC)  xanQ → xanthine:H(+) symporter XanQ
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,078,631 G→A R77C (CGC→TGC)  cmtA ← mannitol‑specific PTS enzyme IICB component CmtA
RA 3,112,839 T→C pseudogene (82/867 nt) yghF ← putative type II secretion system C‑type protein
RA 3,148,135 T→C L80P (CTG→CCG)  gpr → L‑glyceraldehyde 3‑phosphate reductase
RA 3,184,007 T→C T154A (ACT→GCT)  ribB ← 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
RA 3,189,688 A→G pseudogene (2257/2434 nt) yqiG → putative outer membrane usher protein YqiG
RA 3,229,143 T→C Y86H (TAC→CAC)  ygjK → glycoside hydrolase
RA 3,279,346 G→A A145T (GCT→ACT)  kbaZ → tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ
RA 3,283,423 T→G V94G (GTC→GGC)  kbaY → tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaY
RA 3,345,712 T→C S332S (AGT→AGC rpoN → RNA polymerase, sigma 54 (sigma N) factor
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,436,950 G→A V145M (GTG→ATG)  trkA → NAD‑binding component of Trk potassium transporters
RA 3,504,851 T→C intergenic (+68/+84) frlR → / ← yhfS putative DNA‑binding transcriptional regulator FrlR/putative aminotransferase YhfS
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,592,531 G→A intergenic (‑206/+193) ugpB ← / ← livF sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein/branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivF
RA 3,630,072 A→G S177S (TCT→TCC yhiI ← putative membrane fusion protein YhiI
RA 3,650,576 G→A E114K (GAA→AAA)  arsC → arsenate reductase
RA 3,664,969 (T)8→7 intergenic (+1/+17) gadY → / ← gadX small regulatory RNA GadY/DNA‑binding transcriptional dual regulator GadX
RA 3,709,561 (C)6→5 coding (915/1692 nt) eptB ← Kdo2‑lipid A phosphoethanolamine 7''‑transferase
RA 3,751,043 T→C intergenic (+262/+85) sgbE → / ← yiaT L‑ribulose‑5‑phosphate 4‑epimerase SgbE/outer membrane protein YiaT
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 4,085,179 T→G N215T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,101,867 (G)5→4 coding (178/984 nt) kdgT → 2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
RA 4,131,913 A→G V693V (GTA→GTG metL → fused aspartate kinase/homoserine dehydrogenase 2
RA 4,162,148 G→A intergenic (+18/+22) yijD → / ← trmA conserved inner membrane protein YijD/tRNA m(5)U54 methyltransferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,934 G→A S64S (AGC→AGT yjcO ← Sel1 repeat‑containing protein YjcO
RA 4,360,183 A→G intergenic (‑152/+213) cadB ← / ← cadC lysine:cadaverine antiporter/DNA‑binding transcriptional activator CadC
RA 4,370,000 G→A T138I (ACT→ATT)  yjeH ← L‑methionine/branched chain amino acid exporter
RA 4,389,327 (C)6→7 coding (44/3324 nt) mscM ← miniconductance mechanosensitive channel MscM
RA 4,396,129 C→T A22V (GCC→GTC)  amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,424,457 (A)8→9 intergenic (+71/+59) ulaF → / ← yjfY L‑ribulose‑5‑phosphate 4‑epimerase UlaF/DUF1471 domain‑containing protein YjfY
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,462,897 (T)7→8 intergenic (‑237/+157) nrdD ← / ← treC anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑phosphate hydrolase
RA 4,549,854 A→G intergenic (+144/+99) fimH → / ← gntP type 1 fimbriae D‑mannose specific adhesin/fructuronate transporter
RA 4,602,519 A→C Y114D (TAC→GAC)  yjjP ← putative succinate exporter YjjP
RA 4,606,330 (G)8→9 noncoding (72/87 nt) leuQ ← tRNA‑Leu
RA 4,619,892 A→G D97G (GAT→GGT)  deoB → phosphopentomutase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196270 1211436 15167 23 [22] [19] 23 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423765–3424234 3424523–3424239 6–759 23 [22] [13] 28 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)102 (1.220) 58/266 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =141 (1.630)120 (1.400) 65/272 0.1 62.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.050)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)91 (1.050) 54/274 0.3 96.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA