Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	146	75.3	2562504	98.6%	2526628	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,344,150	G→A	L69L (CTG→CTA)	lptB →	lipopolysaccharide transport system ATP binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,344,150	0	G	A	100.0%	200.7 / NA	58	L69L (CTG→CTA)	lptB	lipopolysaccharide transport system ATP binding protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/26); total (32/26)

GACCGTCAACTCCGGGGAAATTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACA  >  NC_000913/3344015‑3344281

gACCGTCAACTCCGGGGAAATTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCtactgc                                                                                                                                 <  2:1129462/140‑1 (MQ=255)

            cGGGGAAATTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTTTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCAc                                                                                                                     >  2:790706/1‑140 (MQ=255)

                 aaaTTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCAcgcgcg                                                                                                                <  2:6226/140‑1 (MQ=255)

                 aaaTTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCAcgcgcg                                                                                                                <  1:1171656/140‑1 (MQ=255)

                      gTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGcgccg                                                                                                           <  1:521945/140‑1 (MQ=255)

                      gTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGcgccg                                                                                                           <  1:168259/140‑1 (MQ=255)

                           tCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTa                                                                                                      >  1:78379/1‑140 (MQ=255)

                              gctgGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCg                                                                                                   >  2:209673/1‑140 (MQ=255)

                                 ggggCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCt                                                                                                <  2:308800/140‑1 (MQ=255)

                                     ccAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCt                                                                                            >  2:206595/1‑140 (MQ=255)

                                         aCGGTGCCGGTAAGACCACCACTTTCTACATTGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCca                                                                                        >  1:1267806/1‑140 (MQ=255)

                                             tGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAgg                                                                                    >  2:883419/1‑140 (MQ=255)

                                                 ggTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGc                                                                                <  1:1106933/140‑1 (MQ=255)

                                                   tAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCt                                                                              <  1:883419/140‑1 (MQ=255)

                                                     aGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTcc                                                                            >  2:961745/1‑140 (MQ=255)

                                                            cacTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTcc                                                                     >  2:401355/1‑140 (MQ=255)

                                                            cacTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTcc                                                                     >  2:280544/1‑140 (MQ=255)

                                                             acTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCg                                                                    <  1:624959/140‑1 (MQ=255)

                                                                   tACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCt                                                              <  1:506649/140‑1 (MQ=255)

                                                                        ggTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCAtt                                                                         <  2:223616/124‑1 (MQ=255)

                                                                        ggTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCAtt                                                                         >  1:223616/1‑124 (MQ=255)

                                                                        ggTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCa                                                            <  2:770212/137‑1 (MQ=255)

                                                                        ggTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCa                                                            >  1:770212/1‑137 (MQ=255)

                                                                        ggTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCg                                                         >  1:1228678/1‑140 (MQ=255)

                                                                         gTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGt                                                        >  2:799467/1‑140 (MQ=255)

                                                                            gTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTa                                                     <  1:280544/140‑1 (MQ=255)

                                                                             tAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTAc                                                    >  2:1221285/1‑140 (MQ=255)

                                                                                gCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGAt                                                 <  1:206595/140‑1 (MQ=255)

                                                                                  aTTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACg                                                   >  1:758417/1‑136 (MQ=255)

                                                                                  aTTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACg                                                   <  2:758417/136‑1 (MQ=255)

                                                                                    tgtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAAcc                                             >  2:985985/1‑140 (MQ=255)

                                                                                    tgtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAAcc                                             >  1:312684/1‑140 (MQ=255)

                                                                                     gtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCt                                            <  2:1267806/140‑1 (MQ=255)

                                                                                     gtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCt                                            <  1:401355/140‑1 (MQ=255)

                                                                                      tgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTg                                           >  2:612526/1‑140 (MQ=255)

                                                                                           cgcgATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGc                                      >  2:786992/1‑140 (MQ=255)

                                                                                           cgcgATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGc                                      >  2:958333/1‑140 (MQ=255)

                                                                                           cgcgATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGc                                      >  1:527992/1‑140 (MQ=255)

                                                                                                        aCATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTACCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAAtt                         >  2:1158983/1‑140 (MQ=255)

                                                                                                          atcatTGTTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCg                       >  1:843672/1‑140 (MQ=255)

                                                                                                          atcatTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCaaa                           <  2:303535/136‑1 (MQ=255)

                                                                                                          atcatTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCaaa                           >  1:303535/1‑136 (MQ=255)

                                                                                                          atcatTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCg                       >  2:454307/1‑140 (MQ=255)

                                                                                                          atcatTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCg                       >  1:271415/1‑140 (MQ=255)

                                                                                                                   gatgatgaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTgac                   >  1:606435/1‑135 (MQ=255)

                                                                                                                   gatgatgaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTgac                   <  2:606435/135‑1 (MQ=255)

                                                                                                                   gatgatgaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACtt              <  1:1036007/140‑1 (MQ=255)

                                                                                                                   gatgatgaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACtt              >  2:544049/1‑140 (MQ=255)

                                                                                                                   gatgatgaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACtt              <  1:544049/140‑1 (MQ=255)

                                                                                                                   gatgatgaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACtt              <  1:209673/140‑1 (MQ=255)

                                                                                                                         gaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTctgc        >  1:972514/1‑140 (MQ=255)

                                                                                                                         gaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTctgc        >  1:917005/1‑140 (MQ=255)

                                                                                                                            gATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGa     <  1:1023188/140‑1 (MQ=255)

                                                                                                                              tatCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac   <  2:527992/140‑1 (MQ=255)

                                                                                                                              tatCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac   <  2:1228678/140‑1 (MQ=255)

                                                                                                                              tatCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac   <  1:786992/140‑1 (MQ=255)

                                                                                                                              tatCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac   >  2:952595/1‑140 (MQ=255)

                                                                                                                              tatCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac   <  2:984520/140‑1 (MQ=255)

                                                                                                                               atCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaaca  >  1:789023/1‑140 (MQ=255)

GACCGTCAACTCCGGGGAAATTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACA  >  NC_000913/3344015‑3344281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A