Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	146	75.3	2562504	98.6%	2526628	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,091,007	C→T	A454T (GCG→ACG)	frvB ←	putative PTS enzyme IIBC component FrvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,091,007	0	C	T	100.0%	193.7 / NA	56	A454T (GCG→ACG)	frvB	putative PTS enzyme IIBC component FrvB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/26); total (30/26)

TCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGC  >  NC_000913/4090873‑4091142

tCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGc                                                                                                                                    >  1:1079532/1‑140 (MQ=255)

   ccgcTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCaa                                                                                                                                 >  1:449513/1‑140 (MQ=255)

      cTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCg                                                                                                                              >  1:1183813/1‑140 (MQ=255)

                   cTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATa                                                                                                                 >  2:188725/1‑140 (MQ=255)

                                ttGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCAcc                                                                                                    >  2:847243/1‑140 (MQ=255)

                                  gCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCgg                                                                                                  <  2:1226325/140‑1 (MQ=255)

                                  gCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCgg                                                                                                  <  1:593820/140‑1 (MQ=255)

                                  gCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCgg                                                                                                  <  2:280786/140‑1 (MQ=255)

                                   cAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGc                                                                                                 <  1:984606/140‑1 (MQ=255)

                                     gTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCgg                                                                                                  >  1:222459/1‑137 (MQ=255)

                                     gTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCgg                                                                                                  <  2:222459/137‑1 (MQ=255)

                                     gTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGa                                                                                               >  1:947663/1‑140 (MQ=255)

                                       aaCTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACa                                                                                             >  1:1019089/1‑140 (MQ=255)

                                       aaCTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACa                                                                                             >  1:908550/1‑140 (MQ=255)

                                        aCTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACAt                                                                                            >  2:886795/1‑140 (MQ=255)

                                        aCTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACAt                                                                                            >  1:1191953/1‑140 (MQ=255)

                                          tCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCa                                                                                          <  2:685115/140‑1 (MQ=255)

                                          tCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCa                                                                                          >  2:712172/1‑140 (MQ=255)

                                             aTGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGc                                                                                       >  2:739276/1‑140 (MQ=255)

                                               gTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCg                                                                                     >  1:445129/1‑140 (MQ=255)

                                                ttCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGa                                                                                    >  2:652724/1‑140 (MQ=255)

                                                   aTGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCa                                                                                 >  1:213549/1‑140 (MQ=255)

                                                    tGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAg                                                                                >  1:491983/1‑140 (MQ=255)

                                                        cAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAg                                                                                <  2:85146/136‑1 (MQ=255)

                                                        cAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAg                                                                                >  1:85146/1‑136 (MQ=255)

                                                         aaGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGt                                                                           <  2:491983/140‑1 (MQ=255)

                                                         aaGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGt                                                                           <  1:847243/140‑1 (MQ=255)

                                                           gCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCa                                                                         >  1:481438/1‑140 (MQ=255)

                                                              ccTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAg                                                                      <  2:1191953/140‑1 (MQ=255)

                                                              ccTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAg                                                                      <  2:947663/140‑1 (MQ=255)

                                                              ccTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAg                                                                      <  2:1183813/140‑1 (MQ=255)

                                                              ccTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAg                                                                      <  1:652724/140‑1 (MQ=255)

                                                                     cgcgGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCa                                                               >  2:447673/1‑140 (MQ=255)

                                                                     cgcgGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCa                                                               <  1:566668/140‑1 (MQ=255)

                                                                       cgGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCaaa                                                             >  1:1131794/1‑140 (MQ=255)

                                                                        ggCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAAc                                                            <  1:712172/140‑1 (MQ=255)

                                                                                                caATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCACACCAGGTGCCAGACGTTTGATTCCGa                                    <  2:445129/140‑1 (MQ=255)

                                                                                                     cTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCg                               <  2:1019089/140‑1 (MQ=255)

                                                                                                     cTTTCAGGACGATAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCg                               <  1:188725/140‑1 (MQ=255)

                                                                                                                aTAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCAc                    <  2:908550/140‑1 (MQ=255)

                                                                                                                aTAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCAc                    <  1:447673/140‑1 (MQ=255)

                                                                                                                 tAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGAt                             >  1:592776/1‑130 (MQ=255)

                                                                                                                 tAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGAt                             <  2:592776/130‑1 (MQ=255)

                                                                                                                 tAATGAATGAAATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACg                   >  2:134412/1‑140 (MQ=255)

                                                                                                                      aatgaaATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGt              >  2:743419/1‑140 (MQ=255)

                                                                                                                         gaaATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCa                     >  2:780428/1‑130 (MQ=255)

                                                                                                                         gaaATGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCa                     <  1:780428/130‑1 (MQ=255)

                                                                                                                          aaaTGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATg          >  1:257158/1‑140 (MQ=255)

                                                                                                                           aaTGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCa                        >  1:228884/1‑125 (MQ=255)

                                                                                                                           aaTGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCa                        <  2:228884/125‑1 (MQ=255)

                                                                                                                           aaTGTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATgc         >  1:1032541/1‑140 (MQ=255)

                                                                                                                              gTTCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATgccgc      >  1:622160/1‑140 (MQ=255)

                                                                                                                                tCAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGccgccc    >  1:226043/1‑140 (MQ=255)

                                                                                                                                 cAGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGccgcccg   <  2:257158/140‑1 (MQ=255)

                                                                                                                                  aGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCCCCGGCGACATCAGGCCGATCAGCGGGTAAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGccgcccgc  <  1:1274982/140‑1 (MQ=255)

                                                                                                                                  aGCGTGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGccgcccgc  <  2:574818/140‑1 (MQ=255)

TCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGC  >  NC_000913/4090873‑4091142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A