Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	153	60.1	2040486	99.3%	2026202	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,957	T→C	E685E (GAA→GAG)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,957	0	T	C	100.0%	159.7 / NA	46	E685E (GAA→GAG)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/26); total (20/26)

AGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGC  >  NC_000913/2819825‑2820091

aGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAgaga                                                                                                                                 <  1:153988/140‑1 (MQ=255)

aGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAgaga                                                                                                                                 <  2:377168/140‑1 (MQ=255)

  ccAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAgaga                                                                                                                                 <  2:762853/138‑1 (MQ=255)

  ccAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAgaga                                                                                                                                 >  1:762853/1‑138 (MQ=255)

       ttattGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCg                                                                                                                          <  1:173247/140‑1 (MQ=255)

         attGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGa                                                                                                                        <  1:165674/140‑1 (MQ=255)

          ttGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGaa                                                                                                                       <  2:21872/140‑1 (MQ=255)

          ttGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGaa                                                                                                                       <  2:266294/140‑1 (MQ=255)

          ttGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGaa                                                                                                                       <  1:772459/140‑1 (MQ=255)

              tATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGa                                                                                                                   <  2:316283/140‑1 (MQ=255)

              tATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGa                                                                                                                   <  1:698267/140‑1 (MQ=255)

               aTCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGAc                                                                                                                  >  2:180741/1‑140 (MQ=255)

                tCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGTACAGAcc                                                                                                                 >  2:669310/1‑140 (MQ=255)

                tCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGTACAGAcc                                                                                                                 <  1:669310/140‑1 (MQ=255)

                tCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGAcc                                                                                                                 >  1:10585/1‑140 (MQ=255)

                tCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGAcc                                                                                                                 >  1:139996/1‑140 (MQ=255)

                        cagcagATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCAc                                                                                                         <  1:180741/140‑1 (MQ=255)

                           cagATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGa                                                                                                                   <  1:716457/127‑1 (MQ=255)

                           cagATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGa                                                                                                                   >  2:716457/1‑127 (MQ=255)

                             gATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTg                                                                                                    <  2:462510/140‑1 (MQ=255)

                              aTGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGc                                                                                                   >  1:181253/1‑140 (MQ=255)

                              aTGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGc                                                                                                   >  2:296324/1‑140 (MQ=255)

                                gcgcGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCgg                                                                                                 <  2:498650/140‑1 (MQ=255)

                                gcgcGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCgg                                                                                                 <  1:240854/140‑1 (MQ=255)

                                  gcgACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCt                                                                                               >  2:344231/1‑140 (MQ=255)

                                          tcTTAAGCGATCGCGGGCTGCATGAACGGTGGGGATAGCGCCTTCTCCGTTTCCCGCTTCGATCCGACGAACGCCGGCAGCAGTCCCCGACTCAGAGATGATGCGGATCAGACCAATATCACCAGTGCGGCTGGCGTGAg                                                                                       <  2:924826/139‑1 (MQ=255)

                                                       cTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACaa                                                                          <  1:592480/140‑1 (MQ=255)

                                                         gTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACt                                                                        <  1:296324/140‑1 (MQ=255)

                                                         gTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCATGAGTACCGCCACACAACt                                                                        <  1:932122/140‑1 (MQ=255)

                                                                   aCGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGaa                                                              <  1:242606/140‑1 (MQ=255)

                                                                         gCGATAGCCCCTTCCCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGcc                                                        <  2:247716/140‑1 (MQ=255)

                                                                                     tctcCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCAc                                            >  1:303910/1‑140 (MQ=255)

                                                                                          ggTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTa                                       >  2:867759/1‑140 (MQ=255)

                                                                                           gTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGcc                                                        <  2:311619/122‑1 (MQ=255)

                                                                                           gTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGcc                                                        >  1:311619/1‑122 (MQ=255)

                                                                                             tACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTAcgc                                    >  2:384434/1‑140 (MQ=255)

                                                                                              aCCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTAcgcg                                   <  2:10585/140‑1 (MQ=255)

                                                                                                cGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCt                                 >  2:28631/1‑140 (MQ=255)

                                                                                                             cgaACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTtctc                    >  2:784309/1‑140 (MQ=255)

                                                                                                               aaCGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGc                  >  1:908196/1‑140 (MQ=255)

                                                                                                                 cGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGc                  <  2:26469/138‑1 (MQ=255)

                                                                                                                 cGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGc                  >  1:26469/1‑138 (MQ=255)

                                                                                                                      gcagcaGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAg           <  2:302631/140‑1 (MQ=255)

                                                                                                                       cagcagTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAgc          <  1:784309/140‑1 (MQ=255)

                                                                                                                              aCCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCg   >  2:558213/1‑140 (MQ=255)

                                                                                                                              aCCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCg   >  1:1012135/1‑140 (MQ=255)

                                                                                                                               cccGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGc  >  1:907988/1‑140 (MQ=255)

AGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGC  >  NC_000913/2819825‑2820091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A