Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	153	60.1	2040486	99.3%	2026202	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,972,315	C→T	T240M (ACG→ATG)	tas →	putative NADP(H)‑dependent aldo‑keto reductase Tas

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,972,315	0	C	T	95.9%	165.1 / ‑0.4	49	coding (719/1041 nt)	tas	putative NADP(H)‑dependent aldo‑keto reductase Tas
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (21/26); minor base . (1/1); total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

CACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCAT  >  NC_000913/2972182‑2972448

cacGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGAc                                                                                                                                 <  2:957777/140‑1 (MQ=255)

cacGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGAc                                                                                                                                 <  1:444466/140‑1 (MQ=255)

    aTCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCggg                                                                                                                             >  1:960660/1‑140 (MQ=255)

     tCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGa                                                                                                                            <  2:960660/140‑1 (MQ=255)

     tCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGa                                                                                                                            <  2:556989/140‑1 (MQ=255)

              tATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCa                                                                                                                   <  1:389792/140‑1 (MQ=255)

                   tCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGTAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCA‑GCTGACCGGGAAATATCTCAATGGTg                                                                                                             >  2:424007/1‑140 (MQ=255)

                   tCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGt                                                                                                              >  2:144429/1‑140 (MQ=255)

                     aCCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGc                                                                                                            >  2:520769/1‑140 (MQ=255)

                                 ccTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTgg                                                                                                <  1:38488/140‑1 (MQ=255)

                                 ccTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTgg                                                                                                <  1:964422/140‑1 (MQ=255)

                                  cTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgc                                                                                               <  2:935666/140‑1 (MQ=255)

                                  cTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgc                                                                                               <  2:510539/140‑1 (MQ=255)

                                  cTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgc                                                                                               <  1:520769/140‑1 (MQ=255)

                                   ttACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgcg                                                                                              >  2:650685/1‑140 (MQ=255)

                                    tACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgcgc                                                                                             <  2:477188/140‑1 (MQ=255)

                                    tACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgcgc                                                                                             <  1:144429/140‑1 (MQ=255)

                                    tACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgcgc                                                                                             <  1:721203/140‑1 (MQ=255)

                                     aCAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGTAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCA‑GCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCAc                                                                                           <  1:424007/140‑1 (MQ=255)

                                     aCAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCa                                                                                            >  2:358529/1‑140 (MQ=255)

                                           tGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAAt                                                                                      >  1:77958/1‑140 (MQ=255)

                                           tGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAAt                                                                                      <  1:275885/140‑1 (MQ=255)

                                            gTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATa                                                                                     <  1:915231/140‑1 (MQ=255)

                                            gTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATa                                                                                     >  2:915231/1‑140 (MQ=255)

                                             ttAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATAc                                                                                    >  1:207172/1‑140 (MQ=255)

                                             ttAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATAc                                                                                    <  1:358529/140‑1 (MQ=255)

                                                   cGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgcgc                                                                                             <  1:380200/125‑1 (MQ=255)

                                                   cGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgcgc                                                                                             >  2:380200/1‑125 (MQ=255)

                                                   cGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATAc                                                                                    >  2:980605/1‑134 (MQ=255)

                                                   cGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATAc                                                                                    <  1:980605/134‑1 (MQ=255)

                                                              aGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCa                                                                   <  1:650685/140‑1 (MQ=255)

                                                                tAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCAcc                                                                 <  2:207172/140‑1 (MQ=255)

                                                                  ggTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCAcc                                                                 >  1:888155/1‑138 (MQ=255)

                                                                  ggTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCAcc                                                                 <  2:888155/138‑1 (MQ=255)

                                                                  ggTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCg                                                               >  2:72774/1‑140 (MQ=255)

                                                                  ggTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCg                                                               >  2:736047/1‑140 (MQ=255)

                                                                  ggTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCg                                                               >  1:637332/1‑140 (MQ=255)

                                                                                      gTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGc                                           >  2:229239/1‑140 (MQ=255)

                                                                                                gTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGt                                 >  2:270123/1‑140 (MQ=255)

                                                                                                 tCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTc                                >  2:14414/1‑140 (MQ=255)

                                                                                                 tCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTc                                >  1:867915/1‑140 (MQ=255)

                                                                                                      ctgctgGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCgcggc                           <  1:14414/140‑1 (MQ=255)

                                                                                                        gctgGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGt                         >  2:827347/1‑140 (MQ=255)

                                                                                                        gctgGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGt                         <  2:867915/140‑1 (MQ=255)

                                                                                                        gctgGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGt                         >  2:501847/1‑140 (MQ=255)

                                                                                                                aTTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGat                 <  2:77958/140‑1 (MQ=255)

                                                                                                                           ggTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACg      >  2:558440/1‑140 (MQ=255)

                                                                                                                             tttCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTc    <  2:123103/140‑1 (MQ=255)

                                                                                                                               tCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCAt  <  1:501847/140‑1 (MQ=255)

CACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCAT  >  NC_000913/2972182‑2972448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A