Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	153	60.1	2040486	99.3%	2026202	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,357,504	T→C	V927A (GTC→GCC)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,357,504	0	T	C	100.0%	91.4 / NA	27	V927A (GTC→GCC)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/10); total (17/10)

GGCGGTAATTCGAACTCCGGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCAC  >  NC_000913/3357374‑3357640

ggcggTAATTCGAACTCCGGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGAt                                                                                                                                 <  1:329402/140‑1 (MQ=255)

 gcggTAATTCGAACTCCGGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGAtt                                                                                                                                >  2:688232/1‑140 (MQ=255)

 gcggTAATTCGAACTCCGGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGAtt                                                                                                                                >  1:582619/1‑140 (MQ=255)

            aaCTCCGGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCa                                                                                                                     <  1:688232/140‑1 (MQ=255)

                       aGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGc                                                                                                          <  2:582619/140‑1 (MQ=255)

                       aGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGc                                                                                                          <  2:31566/140‑1 (MQ=255)

                                            cTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGc                                                                                     >  1:682971/1‑140 (MQ=255)

                                                       acaaAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATaaa                                                                          >  2:292201/1‑140 (MQ=255)

                                                           aGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCa                                                                      >  1:532938/1‑140 (MQ=255)

                                                            gtgtCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCAc                                                                     >  1:676377/1‑140 (MQ=255)

                                                               tCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGcc                                                                  >  2:6583/1‑140 (MQ=255)

                                                                       cAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCg                                                          >  1:379037/1‑140 (MQ=255)

                                                                                        gTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCg                                         >  1:516050/1‑140 (MQ=255)

                                                                                                       cTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACg                          >  1:238474/1‑140 (MQ=255)

                                                                                                       cTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACg                          >  2:616203/1‑140 (MQ=255)

                                                                                                             cGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAg                               >  1:616220/1‑129 (MQ=255)

                                                                                                             cGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAg                               <  2:616220/129‑1 (MQ=255)

                                                                                                             cGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGAtc                    >  1:803392/1‑140 (MQ=255)

                                                                                                                 tCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTccc                <  2:532938/140‑1 (MQ=255)

                                                                                                                  cTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTcccc               >  2:101266/1‑140 (MQ=255)

                                                                                                                          tGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGAt                     <  2:236039/126‑1 (MQ=255)

                                                                                                                          tGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGAt                     >  1:236039/1‑126 (MQ=255)

                                                                                                                          tGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCcgccgccgc       >  2:51939/1‑140 (MQ=255)

                                                                                                                          tGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCcgccgccgc       >  1:451134/1‑140 (MQ=255)

                                                                                                                           gCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGca      <  2:451134/140‑1 (MQ=255)

                                                                                                                           gCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGca      <  1:51939/140‑1 (MQ=255)

                                                                                                                               aCGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGcaccac  <  2:682971/140‑1 (MQ=255)

GGCGGTAATTCGAACTCCGGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCAC  >  NC_000913/3357374‑3357640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A