Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	153	60.1	2040486	99.3%	2026202	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,537,286	T→C	*159Q (TAA→CAA)	greB →	transcription elongation factor GreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,537,286	0	T	C	100.0%	164.0 / NA	47	*159Q (TAA→CAA)	greB	transcription elongation factor GreB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/20); total (27/20)

TAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGG  >  NC_000913/3537153‑3537419

tAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGaa                                                                                                                                 <  2:6989/140‑1 (MQ=255)

             tctATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAAcctc                                                                                                                    <  2:650153/140‑1 (MQ=255)

              ctATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAAcctcc                                                                                                                   >  1:585448/1‑140 (MQ=255)

               tATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGaa                                                                                                                                 <  2:974449/125‑1 (MQ=255)

               tATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGaa                                                                                                                                 >  1:974449/1‑125 (MQ=255)

               tATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAAcctcct                                                                                                                  >  1:732010/1‑140 (MQ=255)

                 tCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGc                                                                                                                >  1:847051/1‑140 (MQ=255)

                       ccccGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGAtgg                                                                                                          >  2:101595/1‑140 (MQ=255)

                         ccGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGAtggct                                                                                                        >  2:151805/1‑140 (MQ=255)

                              ggCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCAt                                                                                                   <  2:847051/140‑1 (MQ=255)

                                         tGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgccagcc                                                                                        >  1:433316/1‑140 (MQ=255)

                                          gCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgccagcca                                                                                       >  1:980341/1‑140 (MQ=255)

                                           cTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGccagccag                                                                                      <  2:433316/140‑1 (MQ=255)

                                            tGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGt                                                                                     <  1:151805/140‑1 (MQ=255)

                                            tGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGt                                                                                     <  2:980341/140‑1 (MQ=255)

                                            tGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGt                                                                                     <  2:385854/140‑1 (MQ=255)

                                            tGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGt                                                                                     <  1:101595/140‑1 (MQ=255)

                                            tGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGt                                                                                     <  1:290552/140‑1 (MQ=255)

                                              aaaaaaGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTcc                                                                                   >  1:918454/1‑140 (MQ=255)

                                                   agaagGCGGCGATCTGGCGGTGGAGAATACCCCTGCCGGGGAACCGAGCTGGTATGCCAATGCTATCGAGTACGAGAAACCGCAAAGAAGAGTCTTAACCTCCTGCCGATTGCGGGCCTTTTTGCCAGCCAGTCCGtata                                                                              >  2:891951/1‑140 (MQ=255)

                                                      agTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACt                                                                           <  2:787073/140‑1 (MQ=255)

                                                             gATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCt                                                                    <  2:27471/140‑1 (MQ=255)

                                                                      gtggtgAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGa                                                           >  2:451669/1‑140 (MQ=255)

                                                                         gtgAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATcc                                                        >  2:843405/1‑140 (MQ=255)

                                                                               cccccTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAg                                                  <  2:881799/139‑1 (MQ=255)

                                                                                 cccTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGAt                                                >  2:236511/1‑140 (MQ=255)

                                                                                   cTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGa                                              >  2:192088/1‑140 (MQ=255)

                                                                                      cgggggAAGCGAGTTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACt                                           <  2:731592/138‑1 (MQ=255)

                                                                                            aaGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAAcc                                     <  1:843405/140‑1 (MQ=255)

                                                                                                gAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCatga                                 >  1:495342/1‑140 (MQ=255)

                                                                                                   cTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCAtgatga                              >  1:564943/1‑140 (MQ=255)

                                                                                                   cTGGTATGTTAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCAtgatga                              <  2:564943/140‑1 (MQ=255)

                                                                                                            tAATGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGt                     >  1:221988/1‑140 (MQ=255)

                                                                                                              aTGCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTc                   >  1:442120/1‑140 (MQ=255)

                                                                                                                gCTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTg                 >  1:870175/1‑140 (MQ=255)

                                                                                                                 cTATCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGc                >  2:978156/1‑140 (MQ=255)

                                                                                                                 cTATCAAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGc                >  2:329158/1‑140 (MQ=255)

                                                                                                                   aTCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCg              <  2:495342/140‑1 (MQ=255)

                                                                                                                    tcaaGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGc             <  1:329158/137‑1 (MQ=255)

                                                                                                                    tCGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGc             >  1:605484/1‑140 (MQ=255)

                                                                                                                     cGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGtt                    <  2:718410/132‑1 (MQ=255)

                                                                                                                     cGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGtt                    >  1:718410/1‑132 (MQ=255)

                                                                                                                     cGAGTACGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCa            >  2:923841/1‑140 (MQ=255)

                                                                                                                           cGTGAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTg      >  2:910211/1‑140 (MQ=255)

                                                                                                                              gAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCgg   >  2:634663/1‑140 (MQ=255)

                                                                                                                              gAAACCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCgg   >  2:299073/1‑140 (MQ=255)

                                                                                                                                 aCCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCggg  >  2:468175/1‑138 (MQ=255)

                                                                                                                                 aCCGCAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCggg  <  1:468175/138‑1 (MQ=255)

TAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGG  >  NC_000913/3537153‑3537419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A