Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	153	60.1	2040486	99.3%	2026202	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,941,273	C→T	A19T (GCT→ACT)	yieP ←	putative transcriptional regulator YieP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,941,273	0	C	T	100.0%	186.6 / NA	53	A19T (GCT→ACT)	yieP	putative transcriptional regulator YieP
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/36); total (17/36)

TTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGCAAAAACGGCAAA  >  NC_000913/3941136‑3941404

ttttGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCa                                                                                                                                   <  2:208994/140‑1 (MQ=255)

 tttGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCtcagtcag                                                                                                                                  <  1:386049/140‑1 (MQ=255)

    gCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAAc                                                                                                                               <  2:378614/140‑1 (MQ=255)

        ttAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATAc                                                                                                                           >  2:36906/1‑140 (MQ=255)

         tAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACg                                                                                                                          <  1:950978/140‑1 (MQ=255)

         tAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACg                                                                                                                          <  2:474939/140‑1 (MQ=255)

         tAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACg                                                                                                                          <  2:870736/140‑1 (MQ=255)

          aaCGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGa                                                                                                                         >  2:856356/1‑140 (MQ=255)

              ttttGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAgg                                                                                                                     <  1:883105/140‑1 (MQ=255)

               tttGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGt                                                                                                                    >  2:945192/1‑140 (MQ=255)

                     cGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCt                                                                                                              <  2:467229/140‑1 (MQ=255)

                        ttCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCtgtg                                                                                                           <  1:36906/140‑1 (MQ=255)

                        ttCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCtgtg                                                                                                           <  2:589963/140‑1 (MQ=255)

                        ttCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCtgtg                                                                                                           <  2:920849/140‑1 (MQ=255)

                          cgcgTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCa                                                                                                         <  1:559804/140‑1 (MQ=255)

                            cgTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCtgtg                                                                                                           >  1:314789/1‑136 (MQ=255)

                            cgTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCtgtg                                                                                                           <  2:314789/136‑1 (MQ=255)

                                 cGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACt                                                                                                  >  2:524971/1‑140 (MQ=255)

                                  gCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACtg                                                                                                 >  2:85837/1‑140 (MQ=255)

                                      tACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACtgttgt                                                                                             >  2:552066/1‑140 (MQ=255)

                                      tACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACtgttgt                                                                                             <  1:107455/140‑1 (MQ=255)

                                       aCGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTtgtg                                                                                            <  1:221291/140‑1 (MQ=255)

                                           ctcactcCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCt                                                                                        >  2:840893/1‑140 (MQ=255)

                                            tcactcCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCtt                                                                                       <  2:456179/140‑1 (MQ=255)

                                             cacgcCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTGTTAAGATCCAATGCGCCCGCTTCTCAGTCAGATCATACGCAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTa                                                                                      <  2:575144/140‑1 (MQ=255)

                                                tcCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTtgtg                                                                                            >  2:878854/1‑131 (MQ=255)

                                                tcCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTtgtg                                                                                            <  1:878854/131‑1 (MQ=255)

                                                 ccAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAgg                                                                                  <  1:809757/140‑1 (MQ=255)

                                                    aaTTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAt                                                                               <  1:300336/140‑1 (MQ=255)

                                                     aTTGCTCGCCCAGCTCAATCTCACTGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAtt                                                                              >  2:58229/1‑140 (MQ=255)

                                                                       tctcACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctttttct                                                            <  2:41619/140‑1 (MQ=255)

                                                                            ccGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTa                                                       >  2:529592/1‑140 (MQ=255)

                                                                             cGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTAt                                                      <  2:679667/140‑1 (MQ=255)

                                                                              gggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTAtt                                                     <  1:58229/140‑1 (MQ=255)

                                                                              gggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTAtt                                                     <  1:552066/140‑1 (MQ=255)

                                                                              gggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTAtt                                                     <  1:85837/140‑1 (MQ=255)

                                                                               ggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATtc                                                    <  1:524971/140‑1 (MQ=255)

                                                                               ggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATtc                                                    <  1:840893/140‑1 (MQ=255)

                                                                               ggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATtc                                                    <  1:529592/140‑1 (MQ=255)

                                                                                    aaTGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTcctcct                                               >  1:262824/1‑140 (MQ=255)

                                                                                     aTGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCtt                                              <  2:857535/140‑1 (MQ=255)

                                                                                         tGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTa                                          <  1:343182/140‑1 (MQ=255)

                                                                                         tGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTa                                          <  1:956616/140‑1 (MQ=255)

                                                                                         tGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTa                                          <  2:463389/140‑1 (MQ=255)

                                                                                                   atatTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAgg                                <  1:586018/140‑1 (MQ=255)

                                                                                                   atatTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAgg                                <  1:945192/140‑1 (MQ=255)

                                                                                                      ttCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAg                             <  2:262824/140‑1 (MQ=255)

                                                                                                         aCCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAgtgt                          >  1:964651/1‑140 (MQ=255)

                                                                                                          ccTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGtgtg                         >  1:593761/1‑140 (MQ=255)

                                                                                                              ttAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTa                     <  1:299772/140‑1 (MQ=255)

                                                                                                                     ccGTTGCGCCAGCTTCTCTGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTCTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGc              >  2:380642/1‑140 (MQ=255)

                                                                                                                     ccGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGc              >  2:856909/1‑140 (MQ=255)

                                                                                                                        ttGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGCaaa           >  2:659067/1‑140 (MQ=255)

                                                                                                                            gcCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGCAAAAACg       <  2:964651/140‑1 (MQ=255)

                                                                                                                                 cTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGCAAAAACGGCaaa  <  2:593761/140‑1 (MQ=255)

TTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGCAAAAACGGCAAA  >  NC_000913/3941136‑3941404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A