Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	153	60.1	2040486	99.3%	2026202	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,206,545	T→G	T330P (ACC→CCC)	purH ←	bifunctional AICAR transformylase/IMP cyclohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,206,545	0	T	G	100.0%	174.7 / NA	50	T330P (ACC→CCC)	purH	bifunctional AICAR transformylase/IMP cyclohydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (25/25); total (25/25)

GCCCCACTGACCGCAGGTCAGAACGCGTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGTTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTGCTTCACAA  >  NC_000913/4206420‑4206679

gCCCCACTGACCGCAGGTCAGAACGCGTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCt                                                                                                                          <  1:847401/140‑1 (MQ=255)

gCCCCACTGACCGCAGGTCAGAACGCGTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCt                                                                                                                          <  2:436175/140‑1 (MQ=255)

gCCCCACTGACCGCAGGTCAGAACGCGTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCt                                                                                                                          <  1:566654/140‑1 (MQ=255)

gCCCCACTGACCGCAGGTCAGAACGCGTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCt                                                                                                                          >  2:579811/1‑140 (MQ=255)

  cccACTGACCGCAGGTCAGAACGCGTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTcg                                                                                                                        <  1:111423/140‑1 (MQ=255)

       tGACCGCAGGTCAGAACGCGTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGtt                                                                                                                   <  1:108814/140‑1 (MQ=255)

            gCAGGTCAGAACGCGTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAgg                                                                                                              >  2:218712/1‑140 (MQ=255)

               ggTCAGAACGCGTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCaa                                                                                                           <  1:218712/140‑1 (MQ=255)

                gTCAGAACGCGTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAat                                                                                                          >  2:550467/1‑140 (MQ=255)

                      aCGCGTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATgcc                                                                                                    <  1:109982/140‑1 (MQ=255)

                        gcgTACGTTGTGTTTGGCGGCGGTGATTTTCAGGGTTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTTGCGGTTAAAGGCAATGATgccgc                                                                                                  <  2:459286/140‑1 (MQ=255)

                        gcgTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATgccgc                                                                                                  <  2:580854/140‑1 (MQ=255)

                          gTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGccgccg                                                                                                >  2:744975/1‑140 (MQ=255)

                               ttCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAatga                                                                                                        >  2:845644/1‑127 (MQ=255)

                               ttCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAatga                                                                                                        <  1:845644/127‑1 (MQ=255)

                                   gTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAg                                                                                       >  2:597415/1‑140 (MQ=255)

                                         cggcggTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTggg                                                                                 <  1:597415/140‑1 (MQ=255)

                                            cggTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCg                                                                              >  2:611251/1‑140 (MQ=255)

                                                      cAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTAcg                                                                    <  1:917666/140‑1 (MQ=255)

                                                         ggCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTAcgcgc                                                                 >  1:924061/1‑140 (MQ=255)

                                                            ttcttcGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGTTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGAt                                                              >  1:122183/1‑140 (MQ=255)

                                                              cttcGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCg                                                            >  2:844460/1‑140 (MQ=255)

                                                               ttcGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGt                                                           <  2:157485/140‑1 (MQ=255)

                                                                      gCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATc                                                    >  2:70867/1‑140 (MQ=255)

                                                                              cgcAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATgg                                            >  2:387295/1‑140 (MQ=255)

                                                                              cgcAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATgg                                            >  2:469433/1‑140 (MQ=255)

                                                                                cAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGaa                                          <  2:924061/140‑1 (MQ=255)

                                                                                          ttCGACAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAg                                >  2:63239/1‑140 (MQ=255)

                                                                                               cAAACTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCAcg                           >  2:305588/1‑140 (MQ=255)

                                                                                                   cTGACGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCAcgccgc                       <  2:926537/140‑1 (MQ=255)

                                                                                                      aCGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCa                             <  1:820998/131‑1 (MQ=255)

                                                                                                      aCGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCa                             >  2:820998/1‑131 (MQ=255)

                                                                                                      aCGAGAAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAg                    >  1:490636/1‑140 (MQ=255)

                                                                                                        gagaAATGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAggg                  >  2:855156/1‑140 (MQ=255)

                                                                                                           aaaTGATGGCCTGTGCGGGTTCCGCATCCAGTTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTg               <  2:122183/140‑1 (MQ=255)

                                                                                                           aaaTGATGGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTg               <  1:70867/140‑1 (MQ=255)

                                                                                                              tgatgGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCg            <  2:428252/140‑1 (MQ=255)

                                                                                                              tgatgGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCg            <  1:844460/140‑1 (MQ=255)

                                                                                                                atgGCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTg          <  1:305588/140‑1 (MQ=255)

                                                                                                                   gCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTGCtt       <  1:469433/140‑1 (MQ=255)

                                                                                                                   gCCTGTGCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTGCtt       <  1:387295/140‑1 (MQ=255)

                                                                                                                        tgCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCg            >  1:243099/1‑130 (MQ=255)

                                                                                                                        tgCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCg            <  2:243099/130‑1 (MQ=255)

                                                                                                                        tgCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTGCtt       >  2:276686/1‑135 (MQ=255)

                                                                                                                        tgCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTGCtt       <  1:276686/135‑1 (MQ=255)

                                                                                                                        tgCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTGCTTca     >  1:655738/1‑137 (MQ=255)

                                                                                                                        tgCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTGCTTca     <  2:655738/137‑1 (MQ=255)

                                                                                                                        tgCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTGCTTCACaa  >  1:483944/1‑140 (MQ=255)

                                                                                                                        tgCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTGCTTCACaa  >  1:40147/1‑140 (MQ=255)

                                                                                                                        tgCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTGCTTCACaa  >  1:357777/1‑140 (MQ=255)

                                                                                                                        tgCGGGTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGCCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTGCTTCACaa  >  2:657213/1‑140 (MQ=255)

GCCCCACTGACCGCAGGTCAGAACGCGTACGTTCTGTTTGGCGGCGGTGATTTTCAGGGCTTCTTCGCTGGCGGACGGCGCAATAATCACTTCGACAAACTGACGAGAAATGATGGCCTGTGCGGTTTCCGCATCCAGCTCGCGGTTAAAGGCAATGATGCCGCCGAATGCGGAGGTTGGGTCGGTTTTGTACGCGCGATCGTAAGCATCAAGAATGGAATTGCCGATAGCCACGCCGCAAGGGTTGGCGTGCTTCACAA  >  NC_000913/4206420‑4206679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A