Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	153	60.1	2040486	99.3%	2026202	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,515,997	C→T	V227V (GTG→GTA)	fecA ←	ferric citrate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,515,997	0	C	T	100.0%	112.6 / NA	33	V227V (GTG→GTA)	fecA	ferric citrate outer membrane transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/16); total (17/16)

TTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCC  >  NC_000913/4515860‑4516128

ttttGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGtactac                                                                                                                                   >  2:326529/1‑140 (MQ=255)

ttttGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGtactac                                                                                                                                   >  2:278280/1‑140 (MQ=255)

     tgAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGCACTACGTCaa                                                                                                                              >  1:815426/1‑140 (MQ=255)

         gagGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGc                                                                                                                          <  2:285096/140‑1 (MQ=255)

            gTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATc                                                                                                                       >  1:620541/1‑140 (MQ=255)

                    gagctgaCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCa                                                                                                                               <  2:495112/124‑1 (MQ=255)

                    gagctgaCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCa                                                                                                                               >  1:495112/1‑124 (MQ=255)

                      gctgaCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAAt                                                                                                                             >  1:284764/1‑124 (MQ=255)

                      gctgaCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAAt                                                                                                                             <  2:284764/124‑1 (MQ=255)

                        tgaCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAg                                                                                                           <  2:751030/140‑1 (MQ=255)

                          aCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCg                                                                                                         >  2:438641/1‑140 (MQ=255)

                               ccACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCg                                                                                                         <  1:8078/135‑1 (MQ=255)

                               ccACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCg                                                                                                         >  2:8078/1‑135 (MQ=255)

                                cACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACgg                                                                                                   <  2:620541/140‑1 (MQ=255)

                                cACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACgg                                                                                                   <  1:438641/140‑1 (MQ=255)

                                 aCGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACggg                                                                                                  <  1:278280/140‑1 (MQ=255)

                                 aCGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGCACTACGTCAATGGCATCCATGTTGCCGAGCGAAACggg                                                                                                  <  2:815426/140‑1 (MQ=255)

                                   gcccgccTCGATACCAAAGTCCTGCGGAATGGTACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAg                                                                                                <  2:473024/140‑1 (MQ=255)

                                               aCCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCt                                                                                    <  1:858550/140‑1 (MQ=255)

                                               aCCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCt                                                                                    >  2:458855/1‑140 (MQ=255)

                                                      tCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTg                                                                             >  1:56958/1‑140 (MQ=255)

                                                                    aCGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGa                                                               <  2:56958/140‑1 (MQ=255)

                                                                                 ttCACCATGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGAt                                                  >  1:300623/1‑140 (MQ=255)

                                                                                  tCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACGACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATg                                                 >  2:950931/1‑140 (MQ=255)

                                                                                  tCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGa                                                               >  1:928487/1‑126 (MQ=255)

                                                                                  tCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGa                                                               <  2:928487/126‑1 (MQ=255)

                                                                                             cccACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAg                                      >  2:860715/1‑140 (MQ=255)

                                                                                                        cGGTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGc                           >  1:94505/1‑140 (MQ=255)

                                                                                                          gTCCGTAACGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCgg                         >  2:607481/1‑140 (MQ=255)

                                                                                                                 aCGCACCGCACCACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGa                  <  1:458855/140‑1 (MQ=255)

                                                                                                                     accgcaccACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGgcg              <  1:860715/140‑1 (MQ=255)

                                                                                                                     accgcaccACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGgcg              <  2:94505/140‑1 (MQ=255)

                                                                                                                       cgcaccACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAGGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGcgcg            >  2:891784/1‑140 (MQ=255)

                                                                                                                       cgcaccACCACCGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGcgcg            >  1:856600/1‑140 (MQ=255)

                                                                                                                                 ccGCGTACTACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGcc  >  2:437030/1‑140 (MQ=255)

TTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCC  >  NC_000913/4515860‑4516128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A