Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	128	54.7	1845674	99.3%	1832754	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,516,058	T→C	Y207C (TAC→TGC)	fecA ←	ferric citrate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,516,058	0	T	C	100.0%	161.9 / NA	45	Y207C (TAC→TGC)	fecA	ferric citrate outer membrane transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/21); total (24/21)

TGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCT  >  NC_000913/4515924‑4516188

tGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAggg                                                                                                                               >  2:192169/1‑140 (MQ=255)

tGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAggg                                                                                                                               >  2:712955/1‑140 (MQ=255)

   cACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCg                                                                                                                            <  2:589695/140‑1 (MQ=255)

      gggTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAg                                                                                                                         <  2:116246/140‑1 (MQ=255)

        gTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAggg                                                                                                                       <  1:889636/140‑1 (MQ=255)

          aaCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGa                                                                                                                     <  1:149715/140‑1 (MQ=255)

             aaaGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACgg                                                                                                                  <  2:584751/140‑1 (MQ=255)

                   caccacGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGc                                                                                                            <  2:818727/140‑1 (MQ=255)

                         gccgccCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTcc                                                                                                       <  1:72944/139‑1 (MQ=255)

                         gccgccCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTcc                                                                                                       >  2:72944/1‑139 (MQ=255)

                               cACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGa                                                                                                <  1:370148/140‑1 (MQ=255)

                               cACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGa                                                                                                <  1:192169/140‑1 (MQ=255)

                                     ctGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCg                                                                                          >  1:194318/1‑140 (MQ=255)

                                        cGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGc                                                                                       >  1:258968/1‑140 (MQ=255)

                                             cGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTg                                                                                  >  2:859804/1‑140 (MQ=255)

                                             cGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTg                                                                                  >  1:816625/1‑140 (MQ=255)

                                                  cgcaccgcacCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAg                                                                             >  1:178605/1‑140 (MQ=255)

                                                     accgcaccACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGgcg                                                                          <  2:105318/140‑1 (MQ=255)

                                                     accgcaccACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGgcg                                                                          >  1:907583/1‑140 (MQ=255)

                                                      ccgcaccACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGgcgc                                                                         <  2:584848/140‑1 (MQ=255)

                                                       cgcaccACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGcgcg                                                                        >  2:69012/1‑140 (MQ=255)

                                                       cgcaccACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGcgcg                                                                        >  2:450631/1‑140 (MQ=255)

                                                        gcaccACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCgg                                                                       <  2:360963/140‑1 (MQ=255)

                                                           ccaccaccGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGtt                                                                    <  2:194318/140‑1 (MQ=255)

                                                              ccaccGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAg                                                                 >  1:458668/1‑140 (MQ=255)

                                                                 ccGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGcc                                                              >  2:757476/1‑140 (MQ=255)

                                                                   gcgTACCACGTCAATGGCATCCATGTTACCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGcccc                                                            <  1:885078/140‑1 (MQ=255)

                                                                    cgTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCg                                                           >  2:893887/1‑140 (MQ=255)

                                                                    cgTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGAGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCg                                                           >  2:34004/1‑140 (MQ=255)

                                                                      tACCACTTCAATGGCATCCATGTCGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGa                                                         >  1:672730/1‑140 (MQ=255)

                                                                      tACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGa                                                         <  2:286005/140‑1 (MQ=255)

                                                                               aaTGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGt                                                <  2:515601/140‑1 (MQ=255)

                                                                                        cATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCa                                       <  2:258968/140‑1 (MQ=255)

                                                                                              gCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGt                                 >  2:43429/1‑140 (MQ=255)

                                                                                                  aGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCt                             >  2:417538/1‑140 (MQ=255)

                                                                                                  aGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCt                             >  2:293899/1‑140 (MQ=255)

                                                                                                    cGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGc                           <  2:448406/140‑1 (MQ=255)

                                                                                                         cGGGAGCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTg                      <  2:590587/140‑1 (MQ=255)

                                                                                                              gCCAGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCgtt                 >  1:690658/1‑140 (MQ=255)

                                                                                                                 aGTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCgttgtt              >  2:914641/1‑140 (MQ=255)

                                                                                                                  gTGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGttt             >  2:818736/1‑140 (MQ=255)

                                                                                                                   tGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGtttt            >  1:757900/1‑140 (MQ=255)

                                                                                                                   tGAAAGCTGCGGCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGtttt            >  1:192372/1‑140 (MQ=255)

                                                                                                                             ggCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCt  <  2:458668/140‑1 (MQ=255)

                                                                                                                             ggCTGACCGCAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCt  <  2:178605/140‑1 (MQ=255)

TGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCT  >  NC_000913/4515924‑4516188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A