Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	112	94.0	3211786	99.3%	3189303	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,692,872	T→C	T789A (ACG→GCG)	bcsA ←	cellulose synthase catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,692,872	0	T	C	100.0%	204.4 / NA	57	T789A (ACG→GCG)	bcsA	cellulose synthase catalytic subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/32); total (25/32)

ACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCA  >  NC_000913/3692736‑3693003

aCCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGcaca                                                                                                                                  >  2:342022/1‑140 (MQ=255)

  cGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACt                                                                                                                                >  1:827743/1‑140 (MQ=255)

   gaagaaGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTg                                                                                                                               >  1:943996/1‑140 (MQ=255)

       aagGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGcaca                                                                                                                           <  2:1513552/140‑1 (MQ=255)

       aagGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGcaca                                                                                                                           >  2:1228998/1‑140 (MQ=255)

         ggCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAATGCACACTGCACaaa                                                                                                                         >  2:987108/1‑140 (MQ=255)

              aaaCTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGa                                                                                                                    >  1:233302/1‑140 (MQ=255)

               aaCTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGat                                                                                                                   <  1:1097213/140‑1 (MQ=255)

               aaCTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGat                                                                                                                   <  2:575409/140‑1 (MQ=255)

               aaCTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGat                                                                                                                   <  1:180065/140‑1 (MQ=255)

                aCTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGata                                                                                                                  <  1:665505/140‑1 (MQ=255)

                    cGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGtt                                                                                                              <  1:1102060/140‑1 (MQ=255)

                    cGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGtt                                                                                                              >  1:569781/1‑140 (MQ=255)

                     gCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATTGATATGTTg                                                                                                             <  1:1108755/140‑1 (MQ=255)

                       cAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCt                                                                                                           <  2:161047/140‑1 (MQ=255)

                          aTGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTggg                                                                                                        >  1:155114/1‑140 (MQ=255)

                              cGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGggtggt                                                                                                    >  1:84807/1‑140 (MQ=255)

                                       cGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCAt                                                                                           >  1:201812/1‑140 (MQ=255)

                                        ggAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCAtt                                                                                          >  1:346992/1‑140 (MQ=255)

                                             ccGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCg                                                                                               >  2:798031/1‑130 (MQ=255)

                                             ccGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCg                                                                                               <  1:798031/130‑1 (MQ=255)

                                             ccGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAAttt                                                                                     <  2:155114/140‑1 (MQ=255)

                                              cGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTc                                                                                    >  2:1056030/1‑140 (MQ=255)

                                                  cTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGcc                                                                                <  1:1579953/140‑1 (MQ=255)

                                                  cTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACCCACACTGCATAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGcc                                                                                <  2:1190984/140‑1 (MQ=255)

                                                   ttCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGccc                                                                               <  1:213549/140‑1 (MQ=255)

                                                    tCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCa                                                                              <  1:1376242/140‑1 (MQ=255)

                                                     cAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCaa                                                                             <  2:233302/140‑1 (MQ=255)

                                                     cAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCaa                                                                             <  2:1392259/140‑1 (MQ=255)

                                                     cAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCaa                                                                             <  1:342022/140‑1 (MQ=255)

                                                          tatCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCa                                                                        >  2:427820/1‑140 (MQ=255)

                                                           atCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCaa                                                                             >  1:1511195/1‑134 (MQ=255)

                                                           atCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCaa                                                                             <  2:1511195/134‑1 (MQ=255)

                                                           atCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCAt                                                                       <  2:1175087/140‑1 (MQ=255)

                                                           atCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCAt                                                                       <  2:1213271/140‑1 (MQ=255)

                                                           atCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCAt                                                                       >  1:41931/1‑140 (MQ=255)

                                                           atCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCAt                                                                       <  1:456972/140‑1 (MQ=255)

                                                               agcagACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCACCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTAcc                                                                   >  2:283933/1‑140 (MQ=255)

                                                                  agACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCAt                                                                >  1:1208431/1‑140 (MQ=255)

                                                                    aCTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCa                                                              <  2:41931/140‑1 (MQ=255)

                                                                      tttCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCAcg                                                            <  2:943996/140‑1 (MQ=255)

                                                                              ggCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCAc                                                    >  1:1161690/1‑140 (MQ=255)

                                                                                        ccGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCggg                                          <  1:1403544/140‑1 (MQ=255)

                                                                                         cGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGa                                         <  1:987108/140‑1 (MQ=255)

                                                                                           ggTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGTGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAg                                       >  2:1281265/1‑140 (MQ=255)

                                                                                            gTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGa                                      <  1:280808/140‑1 (MQ=255)

                                                                                              aGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACg                                    <  1:799921/140‑1 (MQ=255)

                                                                                                cTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTa                                  <  2:201812/140‑1 (MQ=255)

                                                                                                cTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTa                                  <  1:905023/140‑1 (MQ=255)

                                                                                                          agagCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGa                        <  2:1482305/140‑1 (MQ=255)

                                                                                                           gagCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGAc                       <  2:147526/140‑1 (MQ=255)

                                                                                                           gagCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGAc                       >  1:219525/1‑140 (MQ=255)

                                                                                                             gcgcCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACcg                     >  2:649811/1‑140 (MQ=255)

                                                                                                                       aTCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGTGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCa           <  1:1281265/140‑1 (MQ=255)

                                                                                                                        tCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCaa          >  1:511860/1‑140 (MQ=255)

                                                                                                                             aCGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGAt     >  2:911032/1‑140 (MQ=255)

                                                                                                                                ggCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCa  >  2:914260/1‑140 (MQ=255)

                                                                                                                                ggCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCa  >  2:951045/1‑140 (MQ=255)

ACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCA  >  NC_000913/3692736‑3693003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A