Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	151	83.1	2798978	99.4%	2782184	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	643,316	(G)_6→7	coding (1229/1239 nt)	ybdR →	putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	643,310	1	.	G	92.9%	185.8 / 6.6	56	coding (1223/1239 nt)	ybdR	putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR
Reads supporting (aligned to +/- strand): ref base . (2/2); new base G (24/28); total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

TGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑GGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAAC  >  NC_000913/643172‑643440

tGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑g                                                                                                                                     <  1:538781/140‑1 (MQ=255)

tGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑g                                                                                                                                     <  1:292059/140‑1 (MQ=255)

 gCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑gg                                                                                                                                    >  1:769444/1‑140 (MQ=255)

 gCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑gg                                                                                                                                    >  1:142558/1‑140 (MQ=255)

 gCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑gg                                                                                                                                    >  1:964959/1‑140 (MQ=255)

 gCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑gg                                                                                                                                    >  2:1327786/1‑140 (MQ=255)

   cGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAATAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑g                                                                                                                                 <  1:248448/140‑1 (MQ=255)

   cGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑g                                                                                                                                 <  1:689317/140‑1 (MQ=255)

   cGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑g                                                                                                                                 >  1:267588/1‑140 (MQ=255)

   cGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑g                                                                                                                                 <  2:357851/140‑1 (MQ=255)

   cGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑g                                                                                                                                 >  2:689317/1‑140 (MQ=255)

    gTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAACCGGGAGGAGGAGGGCGGAAAGGGGATTTTGGAACCCGGGGAAAAAAGGGCAGAGGCGGGGCAGAAGGGGGTTTCAGGTCTGGTGAATGGGATGC‑C‑ggg‑gg                                                                                                                                >  2:799950/1‑140 (MQ=255)

    gTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gg                                                                                                                                >  2:926892/1‑140 (MQ=255)

      ttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gggg                                                                                                                              <  1:926892/140‑2 (MQ=255)

      ttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gggg                                                                                                                              <  1:766354/140‑2 (MQ=255)

      ttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gggg                                                                                                                              >  1:775396/1‑139 (MQ=255)

      ttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gggg                                                                                                                              <  1:518930/140‑2 (MQ=255)

      ttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gggg                                                                                                                              >  1:893150/1‑139 (MQ=255)

      ttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gggg                                                                                                                              >  1:895822/1‑139 (MQ=255)

      ttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gggg                                                                                                                              >  1:916638/1‑139 (MQ=255)

      ttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gggg                                                                                                                              <  2:142558/140‑2 (MQ=255)

      ttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gggg                                                                                                                              >  1:928869/1‑139 (MQ=255)

      ttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gggg                                                                                                                              >  2:703414/1‑139 (MQ=255)

      ttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gggg                                                                                                                              <  1:25486/140‑2 (MQ=255)

      ttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑gggg                                                                                                                              >  1:1267570/1‑139 (MQ=255)

        gAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑ggggaa                                                                                                                            <  2:700009/140‑4 (MQ=255)

        gAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑ggggaa                                                                                                                            <  2:1316972/140‑4 (MQ=255)

          agagGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑ggg‑ggggaaca                                                                                                                          <  2:815274/140‑6 (MQ=255)

               ccgccCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATg                                                                                                                     <  2:9826/140‑1 (MQ=255)

               ccgccCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATg                                                                                                                     <  1:395907/140‑1 (MQ=255)

                cgccCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGa                                                                                                                    >  2:39158/1‑140 (MQ=255)

                  cccGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATc                                                                                                                  >  2:1340079/1‑140 (MQ=255)

                  cccGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATc                                                                                                                  <  1:1327786/140‑1 (MQ=255)

                    cGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGt                                                                                                                <  2:1055715/140‑1 (MQ=255)

                    cGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGt                                                                                                                <  2:519516/140‑1 (MQ=255)

                                  tttCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTc                                                                                                  >  2:1301236/1‑140 (MQ=255)

                                   ttCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCg                                                                                                 <  1:1066686/140‑1 (MQ=255)

                                   ttCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCg                                                                                                 >  1:1378314/1‑140 (MQ=255)

                                   ttCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCg                                                                                                 >  1:1294433/1‑140 (MQ=255)

                                   ttCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCg                                                                                                 >  1:113123/1‑140 (MQ=255)

                                   ttCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCg                                                                                                 <  2:928869/140‑1 (MQ=255)

                                        aaaaCGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTa                                                                                            <  2:889524/140‑1 (MQ=255)

                                        aaaaCGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTa                                                                                            <  1:1301236/140‑1 (MQ=255)

                                             gTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGa                                                                                       <  1:39158/140‑1 (MQ=255)

                                                  gaggagTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGcc                                                                                  <  2:153996/140‑1 (MQ=255)

                                                  gaggagTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGcc                                                                                  <  2:881952/140‑1 (MQ=255)

                                                        tGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACg                                                                            >  2:464337/1‑140 (MQ=255)

                                                             tAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAAt                                                                       >  1:660943/1‑140 (MQ=255)

                                                               aGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATcg                                                                     <  2:113123/140‑1 (MQ=255)

                                                               aGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATcg                                                                     <  2:1294433/140‑1 (MQ=255)

                                                                  tGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATcgccg                                                                  >  2:1255828/1‑140 (MQ=255)

                                                                  tGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATcgccg                                                                  >  1:948459/1‑140 (MQ=255)

                                                                  tGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATcgccg                                                                  <  2:1382129/140‑1 (MQ=255)

                                                                    aTTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCgccgcc                                                                >  2:1121124/1‑140 (MQ=255)

                                                                    aTTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCgccgcc                                                                <  1:1121124/140‑1 (MQ=255)

                                                                    aTTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCgccgcc                                                                >  1:1088821/1‑140 (MQ=255)

                                                                    aTTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑GGG‑GGGAAACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCgccgcc                                                                >  1:682830/1‑140 (MQ=255)

                                                                           tACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCa                                                         <  1:1255828/140‑1 (MQ=255)

                                                                           tACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCa                                                         <  2:409056/140‑1 (MQ=255)

                                                                            aCCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAg                                                        <  2:964959/140‑1 (MQ=255)

                                                                             cccGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGa                                                       <  1:511210/140‑1 (MQ=255)

                                                                               cGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGaaa                                                     <  1:464337/140‑1 (MQ=255)

                                                                                  tGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCa                                                         >  2:515760/1‑133 (MQ=255)

                                                                                  tGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCa                                                         <  1:515760/133‑1 (MQ=255)

                                                                                    cacaAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTa                                                >  1:635333/1‑140 (MQ=255)

                                                                                      caAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATc                                              <  1:1340079/140‑1 (MQ=255)

                                                                                      aaaaGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGCGG‑GGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATc                                              <  1:851950/139‑1 (MQ=255)

                                                                                       aaaGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCa                                             >  2:405673/1‑140 (MQ=255)

                                                                                        aaGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAg                                            >  1:921340/1‑140 (MQ=255)

                                                                                         aGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAgg                                           <  2:893150/140‑1 (MQ=255)

                                                                                               gagGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTaa                                     <  2:921340/140‑1 (MQ=255)

                                                                                                agGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTaaa                                    <  2:660943/140‑1 (MQ=255)

                                                                                                     gcgcAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCt                               >  1:1384371/1‑140 (MQ=255)

                                                                                                     gcgcAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCt                               >  2:1346878/1‑140 (MQ=255)

                                                                                                      cgcAGAAGGCGGTTTCAGTTCTGGTAAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCtt                              <  1:68780/140‑1 (MQ=255)

                                                                                                                        gTCTGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCca            <  2:1088821/140‑1 (MQ=255)

                                                                                                                         tCTGGTGAATGCGATGC‑C‑GGG‑GGGAAACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCcac           <  2:682830/140‑1 (MQ=255)

                                                                                                                           tGGTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACaa         >  1:572413/1‑140 (MQ=255)

                                                                                                                            ggTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACaaa        >  2:1330934/1‑140 (MQ=255)

                                                                                                                             gTGAATGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACaaac       >  2:1051374/1‑140 (MQ=255)

                                                                                                                             gTGAATGCGATGC‑C‑GGGTGGG‑AACAATATGATCGTCAGGAGTGGTTCTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACaaac       >  2:964659/1‑140 (MQ=255)

                                                                                                                                  tGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACaaacgaaac  >  1:1233597/1‑140 (MQ=255)

                                                                                                                                  tGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACaaacgaaac  >  2:1055281/1‑140 (MQ=255)

                                                                                                                                  tGCGATGC‑CGGGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACaaacgaaac  >  2:1384044/1‑140 (MQ=255)

TGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGC‑C‑GGG‑GGG‑AACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAAC  >  NC_000913/643172‑643440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A