Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 151 83.1 2798978 99.4% 2782184 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 685,941 (C)6→5 coding (729/741 nt) gltJ ← glutamate/aspartate ABC transporter membrane subunit GltJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913685,9360C.98.3% 237.4 / ‑2.7 58coding (734/741 nt)gltJglutamate/aspartate ABC transporter membrane subunit GltJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (24/33);  minor base T (1/0);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGTAATCGA  >  NC_000913/685799‑686065
                                                                                                                                         |                                                                                                                                 
ccccccGCCAGCATCGTCCCCCACAAAATCCCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGccc                                                                                                                                 <  2:1125732/136‑1 (MQ=255)
 tCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGCCAAAGGAACTCCAGCCAAACTCCTACCTTTTTTGcccc                                                                                                                                >  2:213379/1‑140 (MQ=255)
 tCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGcccc                                                                                                                                >  1:257302/1‑140 (MQ=255)
 tCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGcccc                                                                                                                                >  2:71321/1‑140 (MQ=255)
   cccGCCAGCATCGTCCCCCACAAAATCCCAACCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGccccca                                                                                                                              <  2:1336447/139‑2 (MQ=255)
     cGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGcccccatg                                                                                                                            >  1:568741/1‑137 (MQ=255)
                 ccccaaaaaaaTCCCAAACACCACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCcaggcagg                                                                                                               <  2:446991/133‑1 (MQ=255)
                   cccACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCg                                                                                                             <  2:700287/140‑1 (MQ=255)
                       caAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACt                                                                                                         >  2:1284271/1‑140 (MQ=255)
                       caAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACt                                                                                                         >  2:135935/1‑140 (MQ=255)
                        aaaaTACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACtt                                                                                                        <  2:1357950/140‑1 (MQ=255)
                                    ccTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCa                                                                                            <  2:974795/139‑1 (MQ=255)
                                    aCTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCa                                                                                            >  2:750474/1‑140 (MQ=255)
                                    aCTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCa                                                                                            <  2:257302/140‑1 (MQ=255)
                                      tACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGc                                                                                          >  1:1048871/1‑140 (MQ=255)
                                      tACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGc                                                                                          >  2:767100/1‑140 (MQ=255)
                                        ccgccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAAc                                                                                              <  2:537908/134‑1 (MQ=255)
                                        ccgccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAAc                                                                                              >  1:537908/1‑134 (MQ=255)
                                            cgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGtcatc                                                                                    <  1:750474/140‑1 (MQ=255)
                                             gTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCCGGCAGGCGGACCTTACGTTCAAACAGCGtcatcc                                                                                   >  2:129352/1‑139 (MQ=255)
                                             gTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGtcatca                                                                                   <  1:213379/140‑1 (MQ=255)
                                              tGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCAc                                                                                  <  1:1326743/140‑1 (MQ=255)
                                                 cGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAg                                                                               <  1:135935/140‑1 (MQ=255)
                                                 cGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAg                                                                               <  2:109595/140‑1 (MQ=255)
                                                   gTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCTGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCa                                                                             <  2:1205445/140‑1 (MQ=255)
                                                   gTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCa                                                                             <  1:549831/140‑1 (MQ=255)
                                                   gTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCa                                                                             <  1:1149559/140‑1 (MQ=255)
                                                         ttCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATaa                                                                       <  1:655938/140‑1 (MQ=255)
                                                              ggTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCg                                                                  <  1:373308/140‑1 (MQ=255)
                                                              gacagtCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCg                                                                  <  1:950975/135‑1 (MQ=255)
                                                                     aCCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCa                                                           <  1:988886/140‑1 (MQ=255)
                                                                     aCCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCa                                                           <  2:1048871/140‑1 (MQ=255)
                                                                            cGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATa                                                    >  1:1384552/1‑140 (MQ=255)
                                                                            cGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAACGTTAATCAACACATa                                                    >  2:969015/1‑140 (MQ=255)
                                                                              tCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATAcc                                                  >  1:360499/1‑139 (MQ=255)
                                                                              tCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACCCGTACATTATTT‑TCCCCCATGTTGCCAGGCAGGCGAACTTTTCGTTCAACCAGGGTCCTCACCAGCATGATAAAAACGTTAATCAacacccgtc                                                  >  2:950975/1‑135 (MQ=255)
                                                                               cGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGTCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCa                                                           <  1:266742/130‑1 (MQ=255)
                                                                               cGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGTCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCa                                                           >  2:266742/1‑130 (MQ=255)
                                                                               cGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGc                                                 <  1:129352/140‑1 (MQ=255)
                                                                                  gCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAg                                              <  2:1384552/140‑1 (MQ=255)
                                                                                       tatGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTaa                                         <  2:568741/140‑1 (MQ=255)
                                                                                                gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATgg                                      >  1:195555/1‑134 (MQ=255)
                                                                                                gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATgg                                      <  2:195555/134‑1 (MQ=255)
                                                                                                gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCggggg                                >  1:96148/1‑137 (MQ=255)
                                                                                                gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTaa                                >  2:893487/1‑140 (MQ=255)
                                                                                                gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGGaa                                >  1:1295928/1‑140 (MQ=255)
                                                                                                gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGGaa                                >  1:53299/1‑140 (MQ=255)
                                                                                                gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGGaa                                >  1:563527/1‑140 (MQ=255)
                                                                                                 aagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGGaaa                               >  1:17453/1‑140 (MQ=255)
                                                                                                  agggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAAc                              <  2:1162416/140‑1 (MQ=255)
                                                                                                  agggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAAc                              <  1:1284271/140‑1 (MQ=255)
                                                                                                        aaTGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTcc                        <  2:563527/140‑1 (MQ=255)
                                                                                                        aaTGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTcc                        <  1:893487/140‑1 (MQ=255)
                                                                                                        aaTGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTcc                        <  2:924008/140‑1 (MQ=255)
                                                                                                             aaCTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGc                   <  1:767100/140‑1 (MQ=255)
                                                                                                               cTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGCTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAAAGACTCCCCGGCaa                 >  2:1388054/1‑139 (MQ=255)
                                                                                                                  cAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGCAAACGACTCCCAGGCACggg              >  1:1390626/1‑140 (MQ=255)
                                                                                                                   aGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGc             >  1:820628/1‑140 (MQ=255)
                                                                                                                    gTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGGATggggt            >  1:1050030/1‑138 (MQ=255)
                                                                                                                        aaCTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGt        <  1:71321/140‑1 (MQ=255)
                                                                                                                             gTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGTAATCg   <  1:639037/140‑1 (MQ=255)
                                                                                                                              tACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCAt                 <  2:1281843/125‑1 (MQ=255)
                                                                                                                              tACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCAt                 >  1:1281843/1‑125 (MQ=255)
                                                                                                                              tACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGTAATCGa  >  1:742505/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                 
ATCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGTAATCGA  >  NC_000913/685799‑686065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A