Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	151	83.1	2798978	99.4%	2782184	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,338,135	T→C	E109E (GAA→GAG)	mlaD ←	intermembrane phospholipid transport system, substrate binding protein MlaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,338,135	0	T	C	98.4%	433.4 / ‑4.0	123	E109E (GAA→GAG)	mlaD	intermembrane phospholipid transport system, substrate binding protein MlaD
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (60/61); total (61/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 123 total aligned reads displayed.

TACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACC  >  NC_000913/3337999‑3338261

tactacCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTcc                                                                                                                             >  2:1118561/1‑140 (MQ=255)

      cGTAAAGGAACTGACCAATGAGATCTTCCAGTACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGAGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCcagc                                                                                                                       >  2:943599/1‑140 (MQ=255)

       gTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCcagca                                                                                                                      >  2:748133/1‑140 (MQ=255)

       gTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCcag                                                                                                                        >  2:1215119/1‑138 (MQ=255)

       gTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCcag                                                                                                                        <  1:1215119/138‑1 (MQ=255)

        tAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCcagcag                                                                                                                     >  1:299546/1‑140 (MQ=255)

         aaaGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAgg                                                                                                                    <  1:833423/140‑1 (MQ=255)

         aaaGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAgg                                                                                                                    <  1:126877/140‑1 (MQ=255)

           aGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGcc                                                                                                                  <  1:1070241/140‑1 (MQ=255)

            ggAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCg                                                                                                                 >  2:553373/1‑140 (MQ=255)

               aCTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCg                                                                                                                 >  2:759941/1‑137 (MQ=255)

               aCTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCg                                                                                                                 <  1:759941/137‑1 (MQ=255)

               aCTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGaa                                                                                                              >  1:548536/1‑140 (MQ=255)

               aCTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGaa                                                                                                              >  1:1086525/1‑140 (MQ=255)

                cTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAg                                                                                                             <  1:748133/140‑1 (MQ=255)

                cTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAg                                                                                                             >  2:1142900/1‑140 (MQ=255)

                  gACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTa                                                                                                           >  1:891244/1‑140 (MQ=255)

                  gACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTa                                                                                                           >  2:303917/1‑140 (MQ=255)

                   aCCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTAc                                                                                                          >  1:64849/1‑140 (MQ=255)

                   aCCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTAc                                                                                                          >  2:827939/1‑140 (MQ=255)

                     cAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGa                                                                                                        <  2:754872/140‑1 (MQ=255)

                        tGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGa                                                                                                               <  1:602081/130‑1 (MQ=255)

                        tGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATg                                                                                                     >  2:1261948/1‑140 (MQ=255)

                        tGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATg                                                                                                     >  2:509636/1‑140 (MQ=255)

                           gaTCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTc                                                                                                  >  1:141094/1‑140 (MQ=255)

                             tCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGa                                                                                                               >  2:1209162/1‑125 (MQ=255)

                             tCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGa                                                                                                               <  1:1209162/125‑1 (MQ=255)

                             tCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCT‑CCCCAGCAGGCCGGAAGTACGAATGCTCAGc                                                                                               >  2:1111454/1‑140 (MQ=255)

                               ttCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCg                                                                                              >  1:787017/1‑140 (MQ=255)

                               ttCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCg                                                                                              <  2:787017/140‑1 (MQ=255)

                                tCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCT‑CCCCAGCAGGCCGGAAGTACGAATGCTCAGCGaa                                                                                            <  1:1111454/140‑1 (MQ=255)

                                 ccAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGaa                                                                                            <  1:1384406/140‑1 (MQ=255)

                                 ccAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGaa                                                                                            <  2:891244/140‑1 (MQ=255)

                                   aGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACt                                                                                          <  1:1073382/140‑1 (MQ=255)

                                     cACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACttg                                                                                        <  2:420745/140‑3 (MQ=255)

                                     cACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTgg                                                                                        <  2:662937/140‑1 (MQ=255)

                                      aCCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGt                                                                                       <  2:1258370/140‑1 (MQ=255)

                                        cATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTAt                                                                                     <  1:553373/140‑1 (MQ=255)

                                          tCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCt                                                                                   >  1:1000973/1‑140 (MQ=255)

                                             cAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGa                                                                                <  1:143940/140‑1 (MQ=255)

                                              aGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGaa                                                                               <  2:141094/140‑1 (MQ=255)

                                               gACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAt                                                                              <  1:1118561/140‑1 (MQ=255)

                                               gACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAt                                                                              <  2:1164245/140‑1 (MQ=255)

                                               gACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAt                                                                              <  1:1142900/140‑1 (MQ=255)

                                                aCTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAtg                                                                             <  1:599913/140‑1 (MQ=255)

                                                aCTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAtg                                                                             <  1:366503/140‑1 (MQ=255)

                                                 cTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAtgt                                                                            <  1:1150911/140‑1 (MQ=255)

                                                 cTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAtgt                                                                            <  1:397377/140‑1 (MQ=255)

                                                    aGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGt                                                                         >  1:1373684/1‑140 (MQ=255)

                                                    aGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGt                                                                         >  1:502726/1‑140 (MQ=255)

                                                      tgtCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTta                                                                       >  1:982257/1‑140 (MQ=255)

                                                           tGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAt                                                                              >  2:1086839/1‑128 (MQ=255)

                                                           tGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAt                                                                              <  1:1086839/128‑1 (MQ=255)

                                                            gAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACgt                                                                 >  2:881431/1‑140 (MQ=255)

                                                             aaTTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACgtt                                                                <  2:380820/140‑1 (MQ=255)

                                                              aTTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGaa                                                                               <  1:362049/124‑1 (MQ=255)

                                                                  tATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCa                                                           <  2:47097/140‑1 (MQ=255)

                                                                    tCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTta                                                                       <  2:635065/126‑1 (MQ=255)

                                                                    tCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTta                                                                       >  1:635065/1‑126 (MQ=255)

                                                                       ccATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTc                                                      <  1:380630/140‑1 (MQ=255)

                                                                       ccATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTc                                                      >  2:858168/1‑140 (MQ=255)

                                                                       ccATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTc                                                      >  1:319626/1‑140 (MQ=255)

                                                                          tCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAg                                                   >  2:566995/1‑140 (MQ=255)

                                                                            cTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCg                                                 >  1:676901/1‑140 (MQ=255)

                                                                            cTTCAGGATAGCAGTCCCCAGTTCCCGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCg                                                 >  2:1365700/1‑140 (MQ=255)

                                                                               cAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTa                                              >  1:262449/1‑140 (MQ=255)

                                                                                   aTAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTAcgcg                                          <  2:212645/140‑1 (MQ=255)

                                                                                   aTAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTAcgcg                                          <  1:303917/140‑1 (MQ=255)

                                                                                    tAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCg                                                 >  2:1284835/1‑132 (MQ=255)

                                                                                    tAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCg                                                 <  1:1284835/132‑1 (MQ=255)

                                                                                    tAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTAcgcgc                                         <  2:1000973/140‑1 (MQ=255)

                                                                                     aGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTAcgcgcg                                        >  1:650007/1‑140 (MQ=255)

                                                                                     aGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTAcgcgcg                                        >  2:214410/1‑140 (MQ=255)

                                                                                     aGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTAcgcgcg                                        >  2:1230407/1‑140 (MQ=255)

                                                                                      gCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCgg                                       >  1:1146624/1‑140 (MQ=255)

                                                                                       cAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGc                                      <  1:858168/140‑1 (MQ=255)

                                                                                           ccccAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGAt                                  <  2:319626/140‑1 (MQ=255)

                                                                                            cccAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGtt                                               >  2:839630/1‑126 (MQ=255)

                                                                                            cccAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGtt                                               <  1:839630/126‑1 (MQ=255)

                                                                                            cccAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATa                                 >  2:790631/1‑140 (MQ=255)

                                                                                            cccAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATa                                 >  1:1297660/1‑140 (MQ=255)

                                                                                            cccAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATa                                 >  2:1320062/1‑140 (MQ=255)

                                                                                            cccAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATa                                 >  1:29192/1‑140 (MQ=255)

                                                                                              cAGTTCCGAGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAgg                               <  1:943599/140‑1 (MQ=255)

                                                                                                 ttCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGttt                            >  1:1164199/1‑140 (MQ=255)

                                                                                                   ccGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTc                          <  2:676901/140‑1 (MQ=255)

                                                                                                    cGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCg                         >  1:728847/1‑140 (MQ=255)

                                                                                                      ggTCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCggg                       >  1:1301601/1‑140 (MQ=255)

                                                                                                        tCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTc                     <  2:502726/140‑1 (MQ=255)

                                                                                                        tCTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTc                     <  1:827939/140‑1 (MQ=255)

                                                                                                         cTTCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTcc                    <  2:650007/140‑1 (MQ=255)

                                                                                                          ttCAAAACCGACGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCa                   <  2:1301601/140‑1 (MQ=255)

                                                                                                                    aCGTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATc         >  2:731575/1‑140 (MQ=255)

                                                                                                                      gTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCg       <  2:299546/140‑1 (MQ=255)

                                                                                                                      gTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCg       <  2:29192/140‑1 (MQ=255)

                                                                                                                      gTTTAATGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCg       <  2:625228/140‑1 (MQ=255)

                                                                                                                           aTGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCAcc  <  2:548536/140‑1 (MQ=255)

                                                                                                                           aTGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCAcc  <  1:566995/140‑1 (MQ=255)

                                                                                                                           aTGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCAcc  <  1:1155893/140‑1 (MQ=255)

                                                                                                                           aTGCCAGATATTGCTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCAcc  <  2:599174/140‑1 (MQ=255)

TACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACC  >  NC_000913/3337999‑3338261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A