Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	151	83.1	2798978	99.4%	2782184	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,472,004	C→T	G131D (GGC→GAC)	pyrB ←	aspartate carbamoyltransferase catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,472,004	0	C	T	100.0%	373.7 / NA	106	G131D (GGC→GAC)	pyrB	aspartate carbamoyltransferase catalytic subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (49/57); total (49/57)

Only 100 of 110 total aligned reads displayed.

CGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGC  >  NC_000913/4471868‑4472139

cGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCAt                                                                                                                                      <  2:883193/140‑1 (MQ=255)

cGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCAt                                                                                                                                      >  2:1138294/1‑140 (MQ=255)

cGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCAt                                                                                                                                      <  1:1378600/140‑1 (MQ=255)

  ccTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGtcatca                                                                                                                                    <  2:1246062/140‑1 (MQ=255)

     gAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCg                                                                                                                                 <  2:874472/140‑1 (MQ=255)

     gAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCg                                                                                                                                 <  2:362438/140‑1 (MQ=255)

     gAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCg                                                                                                                                 <  2:1276577/140‑1 (MQ=255)

       gTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGc                                                                                                                               >  2:79641/1‑140 (MQ=255)

          aGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCAtt                                                                                                                            <  1:797848/140‑1 (MQ=255)

          aGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCAtt                                                                                                                            <  1:833772/140‑1 (MQ=255)

          aGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCAtt                                                                                                                            <  2:25362/140‑1 (MQ=255)

           gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGCGATTGTCCACACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTc                                                                                                                           <  2:1250741/140‑1 (MQ=255)

           gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCg                                                                                                                                 >  2:894670/1‑134 (MQ=255)

           gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCg                                                                                                                                 <  1:894670/134‑1 (MQ=255)

           gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTc                                                                                                                           <  2:30552/140‑1 (MQ=255)

           gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTc                                                                                                                           <  2:1226250/140‑1 (MQ=255)

           gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTc                                                                                                                           >  1:643233/1‑140 (MQ=255)

                tGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCg                                                                                                                                 <  2:632985/129‑1 (MQ=255)

                tGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCg                                                                                                                                 >  1:632985/1‑129 (MQ=255)

                tGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTAc                                                                                                                      >  1:672965/1‑140 (MQ=255)

                 gAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCAtt                                                                                                                            <  2:294706/133‑1 (MQ=255)

                 gAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCAtt                                                                                                                            >  1:294706/1‑133 (MQ=255)

                      gTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTc                                                                                                                           >  2:211102/1‑129 (MQ=255)

                      gTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTc                                                                                                                           <  1:211102/129‑1 (MQ=255)

                       tGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCTCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACa                                                                                                               >  1:595481/1‑140 (MQ=255)

                       tGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACa                                                                                                               >  1:1308108/1‑140 (MQ=255)

                       tGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACa                                                                                                               >  1:251784/1‑140 (MQ=255)

                       tGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACa                                                                                                               >  2:727788/1‑140 (MQ=255)

                       tGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACa                                                                                                               <  2:884253/140‑1 (MQ=255)

                       tGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAAAAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTc                                                                                                                           >  1:1049184/1‑128 (MQ=255)

                          ggCCATATTTCAGGTCACCAACCATTGCGACGTAGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTg                                                                                                            >  2:364927/1‑140 (MQ=255)

                                 tttCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGaaa                                                                                                     <  2:138586/140‑1 (MQ=255)

                                 tttCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGaaa                                                                                                     <  2:1062142/140‑1 (MQ=255)

                                 tttCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGaaa                                                                                                     <  2:755971/140‑1 (MQ=255)

                                    cAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACt                                                                                                  <  2:438401/140‑1 (MQ=255)

                                      ggTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCg                                                                                                <  2:293456/140‑1 (MQ=255)

                                      ggTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCg                                                                                                <  2:672965/140‑1 (MQ=255)

                                      ggTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCg                                                                                                <  2:700221/140‑1 (MQ=255)

                                      ggTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCg                                                                                                >  1:397113/1‑140 (MQ=255)

                                         cACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTg                                                                                             >  1:1089987/1‑140 (MQ=255)

                                         cACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTg                                                                                             >  2:284325/1‑140 (MQ=255)

                                          accaaccaTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTgg                                                                                            >  2:71899/1‑140 (MQ=255)

                                          accaaccaTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTgg                                                                                            >  2:1331604/1‑140 (MQ=255)

                                               ccaTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAgg                                                                                       <  2:54539/140‑1 (MQ=255)

                                                 aTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGgcg                                                                                     >  2:573935/1‑140 (MQ=255)

                                                 aTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGgcg                                                                                     <  2:1054614/140‑1 (MQ=255)

                                                     cGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGcgcc                                                                                 >  1:1269425/1‑140 (MQ=255)

                                                        cGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCa                                                                              <  1:736588/140‑1 (MQ=255)

                                                        cGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCa                                                                              <  1:1325494/140‑1 (MQ=255)

                                                        cGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCa                                                                              <  1:357969/140‑1 (MQ=255)

                                                        cGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCa                                                                              <  1:175290/140‑1 (MQ=255)

                                                        cGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCa                                                                              <  1:573935/140‑1 (MQ=255)

                                                        cGTGGAGATTGTCCAGACGCCCCTGGGGTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCa                                                                              >  1:1395105/1‑140 (MQ=255)

                                                         gTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCAc                                                                             <  2:1308108/140‑1 (MQ=255)

                                                          tGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCAcc                                                                            >  2:839746/1‑140 (MQ=255)

                                                                  gTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCg                                                                    >  1:816754/1‑140 (MQ=255)

                                                                   tCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCgg                                                                   >  2:118108/1‑140 (MQ=255)

                                                                    ccAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGa                                                                  <  1:1241847/140‑1 (MQ=255)

                                                                    ccAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGATTTGCGTCGGCTGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGa                                                                  >  2:57257/1‑140 (MQ=255)

                                                                       gACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGa                                                               <  1:279553/140‑1 (MQ=255)

                                                                       gACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGa                                                               >  1:615625/1‑140 (MQ=255)

                                                                        aCGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGAc                                                              >  2:840896/1‑140 (MQ=255)

                                                                         cGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACg                                                             <  2:1038201/140‑1 (MQ=255)

                                                                            cccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCAt                                                          <  2:115739/140‑1 (MQ=255)

                                                                            cccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCAt                                                          >  1:1249887/1‑140 (MQ=255)

                                                                            cccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCAt                                                          <  1:62207/140‑1 (MQ=255)

                                                                            cccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTAATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCAt                                                          <  1:320151/140‑1 (MQ=255)

                                                                             ccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTc                                                                         <  1:427278/124‑1 (MQ=255)

                                                                             ccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTc                                                                         >  2:427278/1‑124 (MQ=255)

                                                                             ccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCAt                                                          >  1:1381020/1‑139 (MQ=255)

                                                                             ccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCAt                                                          <  2:1381020/139‑1 (MQ=255)

                                                                             ccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATc                                                         >  1:1031541/1‑140 (MQ=255)

                                                                                  gTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTAt                                                    <  2:615625/140‑1 (MQ=255)

                                                                                       cTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCAt                                               <  2:1089987/140‑1 (MQ=255)

                                                                                             aGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGt                                         <  1:1253661/140‑1 (MQ=255)

                                                                                               tGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACg                                          >  2:885531/1‑137 (MQ=255)

                                                                                               tGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACg                                          <  1:885531/137‑1 (MQ=255)

                                                                                               tGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTaa                                       >  2:260117/1‑140 (MQ=255)

                                                                                                gAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAg                                      >  1:809633/1‑140 (MQ=255)

                                                                                                    aaGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTAGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCt                                  >  1:53357/1‑140 (MQ=255)

                                                                                                         cAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATaa                             >  2:1104598/1‑140 (MQ=255)

                                                                                                         cAGCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATaa                             >  1:561378/1‑140 (MQ=255)

                                                                                                           gCAAGGTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAAcc                           >  1:211890/1‑140 (MQ=255)

                                                                                                               ggTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGaaa                       <  2:561378/140‑1 (MQ=255)

                                                                                                               ggTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGaaa                       <  1:118108/140‑1 (MQ=255)

                                                                                                               ggTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGaaa                       <  1:736242/140‑1 (MQ=255)

                                                                                                               ggTTTGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGaaa                       <  1:408221/140‑1 (MQ=255)

                                                                                                                 tttGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATg                     >  1:329594/1‑140 (MQ=255)

                                                                                                                 tttGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATg                     <  1:79641/140‑1 (MQ=255)

                                                                                                                   tGCGTCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGt                   <  2:306670/140‑1 (MQ=255)

                                                                                                                       tCGGATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCg               >  1:1121775/1‑140 (MQ=255)

                                                                                                                         ggATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGc             >  1:314555/1‑140 (MQ=255)

                                                                                                                          gATGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGcc            >  1:570711/1‑140 (MQ=255)

                                                                                                                           aTGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCa           >  1:557114/1‑140 (MQ=255)

                                                                                                                           aTGTTGGTTGGAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCa           >  2:1368908/1‑140 (MQ=255)

                                                                                                                              ttggttggAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCg        <  1:1244535/140‑1 (MQ=255)

                                                                                                                                    ggAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGc  >  2:758128/1‑140 (MQ=255)

                                                                                                                                    ggAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGc  <  1:364927/140‑1 (MQ=255)

                                                                                                                                    ggAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGc  <  2:1307941/140‑1 (MQ=255)

                                                                                                                                    ggAGTCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGc  <  2:329594/140‑1 (MQ=255)

CGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGC  >  NC_000913/4471868‑4472139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A