Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	128	54.7	1845674	99.3%	1832754	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,473,680	C→T	G125E (GGG→GAG)	bdcA ←	c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,473,680	0	C	T	98.6%	232.9 / ‑4.2	69	G125E (GGG→GAG)	bdcA	c‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (29/39); total (30/39)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01

TGATCCCACGCGGTCCAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCA  >  NC_000913/4473546‑4473815

tGATCCCACGCGGTCCAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCt                                                                                                                                    <  1:396289/140‑1 (MQ=255)

 gATCCCACGCGGTCCAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCtt                                                                                                                                   >  2:352814/1‑140 (MQ=255)

 gATCCCACGCGGTCCAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCtt                                                                                                                                   >  2:456958/1‑140 (MQ=255)

 gATCCCACGCGGTCCAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCtt                                                                                                                                   >  2:547847/1‑140 (MQ=255)

  aTCCCACGCGGTCCAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTc                                                                                                                                  >  2:141827/1‑140 (MQ=255)

  aTCCCACGCGGTCCAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTc                                                                                                                                  >  1:304527/1‑140 (MQ=255)

             tCCAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGcc                                                                                                                       >  1:697051/1‑140 (MQ=255)

             tCCAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGcc                                                                                                                       >  2:57328/1‑140 (MQ=255)

               cagaaTCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCgg                                                                                                                     <  1:271147/137‑1 (MQ=255)

               cAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCATCAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCgg                                                                                                                     <  1:806527/140‑1 (MQ=255)

               cAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCgg                                                                                                                     >  1:380839/1‑140 (MQ=255)

               cAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCgg                                                                                                                     <  2:630103/140‑1 (MQ=255)

               cAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCgg                                                                                                                     <  2:399680/140‑1 (MQ=255)

                 aaaTCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGggc                                                                                                                   <  2:443976/140‑1 (MQ=255)

                 aaaTCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGggc                                                                                                                   <  1:136319/140‑1 (MQ=255)

                 aaaTCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGAAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGggc                                                                                                                   <  2:305214/140‑1 (MQ=255)

                    tCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGggcggc                                                                                                                <  1:141827/140‑1 (MQ=255)

                       cGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTc                                                                                                             >  2:833510/1‑140 (MQ=255)

                       cGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTc                                                                                                             >  1:811024/1‑140 (MQ=255)

                            cAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACag                                                                                                        <  1:263943/140‑1 (MQ=255)

                            cAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACag                                                                                                        <  1:546531/140‑1 (MQ=255)

                             aGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACaga                                                                                                       >  2:674847/1‑140 (MQ=255)

                              gCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCTGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACagag                                                                                                      <  2:761019/140‑1 (MQ=255)

                              gCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACagag                                                                                                      <  2:811024/140‑1 (MQ=255)

                              gCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACagag                                                                                                      <  2:380839/140‑1 (MQ=255)

                              gCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACagag                                                                                                      <  2:102393/140‑1 (MQ=255)

                              gCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACagag                                                                                                      <  1:784332/140‑1 (MQ=255)

                              gCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACagag                                                                                                      <  1:167990/140‑1 (MQ=255)

                                ccGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACTGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGc                                                                                                    <  1:453689/140‑1 (MQ=255)

                                ccGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGc                                                                                                    <  2:687324/140‑1 (MQ=255)

                                      gcCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATa                                                                                              <  1:883020/140‑1 (MQ=255)

                                      gcCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATa                                                                                              <  2:894644/140‑1 (MQ=255)

                                      gcCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATa                                                                                              <  2:697051/140‑1 (MQ=255)

                                         aTGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAgg                                                                                           <  1:813335/140‑1 (MQ=255)

                                           gCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAg                                                                                         <  1:352814/140‑1 (MQ=255)

                                             cTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCa                                                                                       <  1:890155/140‑1 (MQ=255)

                                                  aGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAat                                                                                  >  2:418022/1‑140 (MQ=255)

                                                  aGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAat                                                                                  >  1:885282/1‑140 (MQ=255)

                                                      cAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTg                                                                              >  1:772348/1‑140 (MQ=255)

                                                      cAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTg                                                                              >  2:743595/1‑140 (MQ=255)

                                                            tGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTg                                                                        <  1:141357/140‑1 (MQ=255)

                                                                 gcggcATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAg                                                                   <  1:491185/140‑1 (MQ=255)

                                                                 gcggcATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAg                                                                   >  1:161852/1‑140 (MQ=255)

                                                                 gcggcATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAg                                                                   <  1:57328/140‑1 (MQ=255)

                                                                   ggcATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGc                                                                 <  2:706696/140‑1 (MQ=255)

                                                                    gcATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCg                                                                <  1:418022/140‑1 (MQ=255)

                                                                             gcCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATAtc                                                       >  2:232134/1‑140 (MQ=255)

                                                                               cacgCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATAtcgt                                                     <  2:52725/137‑1 (MQ=255)

                                                                               cATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATAtcgt                                                     <  2:449164/140‑1 (MQ=255)

                                                                                     tGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCAt                                               >  2:103809/1‑140 (MQ=255)

                                                                                       cAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCAttt                                             <  1:547847/140‑1 (MQ=255)

                                                                                       cAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCAttt                                             <  2:304527/140‑1 (MQ=255)

                                                                                       cAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCAttt                                             >  1:74371/1‑140 (MQ=255)

                                                                                                tACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAgg                                    <  2:94861/140‑1 (MQ=255)

                                                                                                 aCGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAggg                                   >  1:772555/1‑140 (MQ=255)

                                                                                                      cGCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCg                              >  2:552431/1‑140 (MQ=255)

                                                                                                       gCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGc                             >  1:380986/1‑140 (MQ=255)

                                                                                                       gCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGc                             >  1:618514/1‑140 (MQ=255)

                                                                                                       gCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGc                             >  1:885229/1‑140 (MQ=255)

                                                                                                       gCCATTCACGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGc                             >  1:835038/1‑140 (MQ=255)

                                                                                                               cGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACg                     >  1:514869/1‑140 (MQ=255)

                                                                                                               cGGAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACg                     >  2:29690/1‑140 (MQ=255)

                                                                                                                 gAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGcc                   >  2:672296/1‑140 (MQ=255)

                                                                                                                 gAGCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGcc                   <  1:103809/140‑1 (MQ=255)

                                                                                                                   gCCGATGATTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCaa                 >  2:571146/1‑140 (MQ=255)

                                                                                                                       atgatTAAGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATAcc             >  2:332090/1‑140 (MQ=255)

                                                                                                                             aaGATGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCAtt       <  2:813293/140‑1 (MQ=255)

                                                                                                                                aTGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATTCCTGCAtttgc    <  2:772348/140‑3 (MQ=255)

                                                                                                                                 tGCGCTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTacc   <  2:393778/140‑1 (MQ=255)

                                                                                                                                  gcgcTCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTacca  <  1:674847/140‑1 (MQ=255)

TGATCCCACGCGGTCCAAAATCACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCA  >  NC_000913/4473546‑4473815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A