Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	151	83.1	2798978	99.4%	2782184	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,777	A→G	V25A (GTC→GCC)	yjiM ←	putative dehydratase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,777	0	A	G	100.0%	332.5 / NA	91	V25A (GTC→GCC)	yjiM	putative dehydratase subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (36/55); total (36/55)

TCTCCGCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGACGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAG  >  NC_000913/4565640‑4565913

tctcCGCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCg                                                                                                                                         >  1:788659/1‑140 (MQ=255)

tctcCGCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCg                                                                                                                                         >  2:70993/1‑140 (MQ=255)

  tcCGCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGt                                                                                                                                       >  2:439496/1‑140 (MQ=255)

     gCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTgag                                                                                                                                    >  2:1007598/1‑140 (MQ=255)

     gCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTgag                                                                                                                                    <  2:952648/140‑1 (MQ=255)

      cttcttCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTgaga                                                                                                                                   <  2:1104704/140‑1 (MQ=255)

       ttcttcAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGaa                                                                                                                                  >  1:1278094/1‑140 (MQ=255)

          ttcAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGc                                                                                                                               >  2:988418/1‑140 (MQ=255)

           tcAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGcc                                                                                                                              <  1:1007598/140‑1 (MQ=255)

              aTGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGt                                                                                                                           >  1:798827/1‑140 (MQ=255)

              aTGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGt                                                                                                                           <  2:640289/140‑1 (MQ=255)

                   ttCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGc                                                                                                                      >  2:62500/1‑140 (MQ=255)

                   ttCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGc                                                                                                                      <  2:549699/140‑1 (MQ=255)

                      atcaGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAg                                                                                                                   <  2:779740/140‑1 (MQ=255)

                      atcaGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAg                                                                                                                   <  1:810867/140‑1 (MQ=255)

                      atcaGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAg                                                                                                                   <  1:1084025/140‑1 (MQ=255)

                           agGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCa                                                                                                              >  1:1027111/1‑140 (MQ=255)

                           agGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCa                                                                                                              <  2:951972/140‑1 (MQ=255)

                           agGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGAGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGGGAGAAAGCCTGTCTGGCGAGCTTCa                                                                                                              >  2:591315/1‑140 (MQ=255)

                            ggTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCag                                                                                                             >  1:1068116/1‑140 (MQ=255)

                            ggTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCag                                                                                                             >  1:474195/1‑140 (MQ=255)

                             gTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCaga                                                                                                            <  1:947114/140‑1 (MQ=255)

                             gTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCaga                                                                                                            >  2:1209471/1‑140 (MQ=255)

                             gTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCaga                                                                                                            >  1:646768/1‑140 (MQ=255)

                             gTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCaga                                                                                                            >  1:1084594/1‑140 (MQ=255)

                              tGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCagag                                                                                                           <  2:539923/140‑1 (MQ=255)

                                gAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGaa                                                                                                         <  2:795214/140‑1 (MQ=255)

                                gAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGaa                                                                                                         >  1:694683/1‑140 (MQ=255)

                                gAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGaa                                                                                                         >  2:906076/1‑140 (MQ=255)

                                gAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGaa                                                                                                         <  2:656845/140‑1 (MQ=255)

                                     gagCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGAt                                                                                                    >  2:134364/1‑140 (MQ=255)

                                     gagCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGAt                                                                                                    >  2:970399/1‑140 (MQ=255)

                                     gagCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGAt                                                                                                    >  1:775477/1‑140 (MQ=255)

                                      agCGAAACCACAACCGCACCGACTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATc                                                                                                   >  2:993564/1‑140 (MQ=255)

                                       gCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCg                                                                                                  >  1:345307/1‑140 (MQ=255)

                                       gCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCg                                                                                                  >  2:102050/1‑140 (MQ=255)

                                       gCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCg                                                                                                  >  2:1001155/1‑140 (MQ=255)

                                       gCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCg                                                                                                  >  1:1020783/1‑140 (MQ=255)

                                       gCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCg                                                                                                  >  2:977660/1‑140 (MQ=255)

                                        cGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGa                                                                                                 >  2:621048/1‑140 (MQ=255)

                                        cGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGa                                                                                                 >  2:865855/1‑140 (MQ=255)

                                          aaaCCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGaaa                                                                                               <  2:694683/140‑1 (MQ=255)

                                           aaCCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGaaaa                                                                                              >  1:489849/1‑140 (MQ=255)

                                             ccACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATg                                                                                            <  1:906076/140‑1 (MQ=255)

                                             ccACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATg                                                                                            <  1:1243487/140‑1 (MQ=255)

                                                  aCCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCggg                                                                                       >  2:824410/1‑140 (MQ=255)

                                                  aCCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCggg                                                                                       <  2:94908/140‑1 (MQ=255)

                                                   ccGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGt                                                                                      <  1:977660/140‑1 (MQ=255)

                                                    cGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTa                                                                                     <  1:11231/140‑1 (MQ=255)

                                                      cACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGa                                                                                   <  1:70993/140‑1 (MQ=255)

                                                          ggCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCgg                                                                               <  1:62500/140‑1 (MQ=255)

                                                           gCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGt                                                                              <  2:244108/140‑1 (MQ=255)

                                                              gCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGAc                                                                           <  2:1068116/140‑1 (MQ=255)

                                                              gCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGAc                                                                           <  1:330011/140‑1 (MQ=255)

                                                              gCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGAc                                                                           <  2:1020783/140‑1 (MQ=255)

                                                                      gATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACa                                                                   <  2:798827/140‑1 (MQ=255)

                                                                              gCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTttc                                                          <  2:688601/140‑1 (MQ=255)

                                                                               cGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGAGATCGGGGACAAGTGACAAGA‑tttttc                                                         >  1:138162/1‑138 (MQ=255)

                                                                                    tAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTccc                                                    <  2:738075/140‑1 (MQ=255)

                                                                                      aaGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCg                                                  <  2:611153/140‑1 (MQ=255)

                                                                                      aaGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCg                                                  <  1:93270/140‑1 (MQ=255)

                                                                                        ggTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTa                                                <  2:489849/140‑1 (MQ=255)

                                                                                           gCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCa                                             <  1:1133992/140‑1 (MQ=255)

                                                                                             aGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACg                                           <  2:775477/140‑1 (MQ=255)

                                                                                                 aGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGgag                                       <  1:42742/140‑1 (MQ=255)

                                                                                                 aGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGAGATCGGGGGCAAAAGACATGA‑TTTTTCTTTCCCCCACCACggggg                                       >  2:42742/1‑138 (MQ=255)

                                                                                                 aGTGCCAACCAGCGGAATGCCGAGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGAGATCGGGGGCAAGGGACATGA‑TTTTTCTTCCCCCTACCACcgggg                                       >  2:89263/1‑138 (MQ=255)

                                                                                                   tGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGCGATTGGGGGCAAGGGGAAAGAGTTTTCTTTCCCCTACCCACGGggga                                      >  2:1098616/1‑140 (MQ=255)

                                                                                                      cAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAAt                                  <  2:812489/140‑1 (MQ=255)

                                                                                                      cAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACACGA‑TTTTCCTTCCCCGTACCACGGGAGATAAt                                  <  2:1278094/140‑1 (MQ=255)

                                                                                                          cAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGaaa                              <  1:667030/140‑1 (MQ=255)

                                                                                                           aGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGaaaa                             <  1:455250/140‑1 (MQ=255)

                                                                                                            gCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAag                            <  1:1098616/140‑1 (MQ=255)

                                                                                                                aaTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGc                        <  1:824410/140‑1 (MQ=255)

                                                                                                                aaTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGc                        <  1:1001155/140‑1 (MQ=255)

                                                                                                                aaTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGc                        <  2:407643/140‑1 (MQ=255)

                                                                                                                aaTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGc                        <  2:1027111/140‑1 (MQ=255)

                                                                                                                aaTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGc                        <  1:716063/140‑1 (MQ=255)

                                                                                                                aaTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGc                        <  1:865855/140‑1 (MQ=255)

                                                                                                                 aTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGcc                       <  1:465318/140‑1 (MQ=255)

                                                                                                                 aTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGcc                       <  2:345307/140‑1 (MQ=255)

                                                                                                                  tGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCca                      <  1:621048/140‑1 (MQ=255)

                                                                                                                  tGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCca                      <  1:993564/140‑1 (MQ=255)

                                                                                                                     cgcgCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACat                   <  1:134364/140‑1 (MQ=255)

                                                                                                                       cgcTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGAAATTTGGGGGAAAGGACATGA‑TTTTTCTTCCCCCCCCCACGGGAGATAATGAAAAGAGGGCACcttt                 >  2:716063/1‑139 (MQ=255)

                                                                                                                             ttGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCa           <  1:325711/140‑1 (MQ=255)

                                                                                                                             ttGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCa           <  2:474195/140‑1 (MQ=255)

                                                                                                                                    ccATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCtttt    <  2:646768/140‑1 (MQ=255)

                                                                                                                                    ccATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCtttt    <  2:1084594/140‑1 (MQ=255)

                                                                                                                                      aTGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAg  <  1:518089/140‑1 (MQ=255)

                                                                                                                                      aTGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGAGAGCGGTGAAAAGAGGCATGA‑TTTTTCTTCCCCCCTTCACCCGAGGTAATAAAAAAAGGGGACAGTTTTACCATtttttttt  >  2:73318/1‑138 (MQ=255)

TCTCCGCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGACGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAG  >  NC_000913/4565640‑4565913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A