Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	138	91.1	3071990	99.2%	3047414	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,001,665	T→C	Y441H (TAC→CAC)	xdhA →	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,001,665	0	T	C	100.0%	144.5 / NA	42	Y441H (TAC→CAC)	xdhA	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/24); total (18/24)

GCAAACGTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCTACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTCAGGGTGCCGACACCGT  >  NC_000913/3001528‑3001786

gCAAACGTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCac                                                                                                                         <  1:490065/140‑1 (MQ=255)

 cAAACGTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCcaca                                                                                                                        <  2:196710/140‑1 (MQ=255)

 cAAACGTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCcaca                                                                                                                        <  1:988543/140‑1 (MQ=255)

 cAAACGTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCcaca                                                                                                                        <  1:35796/140‑1 (MQ=255)

      gTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACctct                                                                                                                   <  2:1361455/140‑1 (MQ=255)

              aGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTg                                                                                                           >  1:264954/1‑140 (MQ=255)

                cgcAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGc                                                                                                         >  2:990367/1‑140 (MQ=255)

                cgcAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGc                                                                                                         >  2:533589/1‑140 (MQ=255)

                  cAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCt                                                                                                       >  2:706759/1‑140 (MQ=255)

                    gggTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGt                                                                                                     <  2:1041612/140‑1 (MQ=255)

                     ggTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTaa                                                                                                                 <  1:1248305/127‑1 (MQ=255)

                     ggTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTaa                                                                                                                 >  2:1248305/1‑127 (MQ=255)

                       ttGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCgg                                                                                                  <  1:1014889/140‑1 (MQ=255)

                       ttGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCgg                                                                                                  <  1:990367/140‑1 (MQ=255)

                        tGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGc                                                                                                 <  2:992156/140‑1 (MQ=255)

                          ccGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGt                                                                                               >  1:390794/1‑140 (MQ=255)

                            ggAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAg                                                                                             >  2:468807/1‑140 (MQ=255)

                               gtgtCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGaaa                                                                                          >  1:1490467/1‑140 (MQ=255)

                                tgtCTTGAAAAAGGCCGGAAAATCTTTTAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAAt                                                                                         >  2:1421512/1‑140 (MQ=255)

                                tgtCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAAt                                                                                         >  2:1076617/1‑140 (MQ=255)

                                          aaGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGgcgcg                                                                               <  2:1490467/140‑1 (MQ=255)

                                           aGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGgcgcgc                                                                              >  2:685177/1‑140 (MQ=255)

                                           aGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGgcgcgc                                                                              <  1:1094679/140‑1 (MQ=255)

                                              ccGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGcc                                                                           <  1:105124/140‑1 (MQ=255)

                                              ccGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGcc                                                                           <  2:84798/140‑1 (MQ=255)

                                              ccGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGcc                                                                           <  1:468807/140‑1 (MQ=255)

                                                    aaTCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCtga                                                                     <  1:533589/140‑1 (MQ=255)

                                                         ttGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAAt                                                                <  2:390794/140‑1 (MQ=255)

                                                         ttGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAAt                                                                <  1:706759/140‑1 (MQ=255)

                                                                ggAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATg                                                         >  2:96628/1‑140 (MQ=255)

                                                                 gAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATgg                                                        <  1:1421512/140‑1 (MQ=255)

                                                                    aaaCGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAAc                                                     >  1:153687/1‑140 (MQ=255)

                                                                      aCGCCGTGCGGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGAGCCTTCTGATGAATCAGGATGGAACCa                                                   <  2:508591/140‑1 (MQ=255)

                                                                       cGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCAt                                                  <  2:153687/140‑1 (MQ=255)

                                                                           gTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGAt                                                          >  2:846596/1‑128 (MQ=255)

                                                                           gTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGAt                                                          <  1:846596/128‑1 (MQ=255)

                                                                                           ccAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGa                              >  1:1110477/1‑140 (MQ=255)

                                                                                           ccAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAAAACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGa                              >  1:1415492/1‑140 (MQ=255)

                                                                                                   gCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATc                      >  1:1090219/1‑140 (MQ=255)

                                                                                                    cAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCg                     >  2:1384305/1‑140 (MQ=255)

                                                                                                       tttGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTc                  <  1:1384305/140‑1 (MQ=255)

                                                                                                        ttGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTCa                 <  1:1076617/140‑1 (MQ=255)

                                                                                                           cgccgcGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTCAggg              <  2:1090219/140‑1 (MQ=255)

                                                                                                             ccgcGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAAAACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTCAGGGTg            <  2:1415492/140‑1 (MQ=255)

                                                                                                                       ggCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTCAGGGTGCCGACACCGt  <  2:402069/140‑1 (MQ=255)

GCAAACGTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCTACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTCAGGGTGCCGACACCGT  >  NC_000913/3001528‑3001786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A