Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	138	91.1	3071990	99.2%	3047414	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,673,639	(G)_5→6	coding (278/1014 nt)	yhjD →	putative transporter YhjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,673,634	1	.	G	98.9%	315.2 / ‑3.7	92	coding (273/1014 nt)	yhjD	putative transporter YhjD
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (43/48); total (43/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 102 total aligned reads displayed.

CCAACGCCAACCGGTTATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑A‑‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACACCGCCGTTCAGCAGCGTACGA  >  NC_000913/3673496‑3673767

ccAACGCCAACCGGTTATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑A‑‑g                                                                                                                                      >  2:1326183/1‑140 (MQ=255)

  aaCGCCAACCGGTTATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑A‑‑ggg                                                                                                                                    <  2:1110224/140‑1 (MQ=255)

  aaCGCCAACCGGTTATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑A‑‑ggg                                                                                                                                    >  1:675113/1‑140 (MQ=255)

  aaCGCCAACCGGTTATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑A‑‑ggg                                                                                                                                    <  1:1281235/140‑1 (MQ=255)

       cAACCGGTTATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑A‑‑ggggggct                                                                                                                               <  2:828038/140‑4 (MQ=255)

       cAACCGGTTATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑A‑‑ggggggct                                                                                                                               <  1:1326183/140‑4 (MQ=255)

         aCCGGTTATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑A‑‑gggggggctt                                                                                                                             >  2:1338049/1‑135 (MQ=255)

         aCCGGTTATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑A‑‑ggg                                                                                                                                    <  2:259063/133‑1 (MQ=255)

         aCCGGTTATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑A‑‑ggg                                                                                                                                    >  1:259063/1‑133 (MQ=255)

            ggTTATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTtgtg                                                                                                                           <  1:284075/140‑1 (MQ=255)

              ttATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCt                                                                                                                         >  1:1016413/1‑140 (MQ=255)

              ttATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCt                                                                                                                         <  1:1298693/140‑1 (MQ=255)

              ttATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCt                                                                                                                         <  1:246776/140‑1 (MQ=255)

                aTTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTgg                                                                                                                       >  2:504391/1‑140 (MQ=255)

                    cgcACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTc                                                                                                                   >  2:1415558/1‑140 (MQ=255)

                       aCCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCa                                                                                                                >  2:614133/1‑140 (MQ=255)

                       aCCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCa                                                                                                                >  2:1173926/1‑140 (MQ=255)

                        tcTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCAt                                                                                                               <  1:1157504/139‑1 (MQ=255)

                        ccTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCAt                                                                                                               <  1:1341949/140‑1 (MQ=255)

                        ccTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCAt                                                                                                               >  1:69199/1‑140 (MQ=255)

                          tGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATcc                                                                                                             <  1:1415558/140‑1 (MQ=255)

                          tGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATcc                                                                                                             <  1:1278440/140‑1 (MQ=255)

                            aTTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGa                                                                                                           <  2:576738/140‑1 (MQ=255)

                            aTTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGa                                                                                                           <  1:514070/140‑1 (MQ=255)

                              tCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATg                                                                                                         <  2:1223092/140‑1 (MQ=255)

                               cgcgcgACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGt                                                                                                        <  2:1016413/140‑1 (MQ=255)

                                 cgcgACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGc                                                                                                                      >  2:1157822/1‑124 (MQ=255)

                                 cgcgACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGc                                                                                                                      <  1:1157822/124‑1 (MQ=255)

                                 cgcgACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATcc                                                                                                             <  2:1484812/133‑1 (MQ=255)

                                 cgcgACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATcc                                                                                                             >  1:1484812/1‑133 (MQ=255)

                                 cgcgACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTg                                                                                                      >  2:64531/1‑140 (MQ=255)

                                  gcgACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGc                                                                                                     <  2:895809/140‑1 (MQ=255)

                                    gACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTa                                                                                                   <  2:385087/140‑1 (MQ=255)

                                      cAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACa                                                                                                 >  1:1067614/1‑140 (MQ=255)

                                              tttAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCt                                                                                         >  1:54409/1‑140 (MQ=255)

                                                tAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTc                                                                                       >  2:1504375/1‑140 (MQ=255)

                                                tAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTc                                                                                       >  2:191874/1‑140 (MQ=255)

                                                      tCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACaaa                                                                                 >  2:331917/1‑140 (MQ=255)

                                                       cGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACaaaa                                                                                >  2:1332856/1‑140 (MQ=255)

                                                        ggCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAAt                                                                               >  2:1382283/1‑140 (MQ=255)

                                                                  ccAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAAc                                                                     >  2:1081229/1‑140 (MQ=255)

                                                                  ccAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAAc                                                                     >  2:712902/1‑140 (MQ=255)

                                                                       ttGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAg                                                                >  2:1086104/1‑140 (MQ=255)

                                                                       ttGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAg                                                                >  2:1066021/1‑140 (MQ=255)

                                                                       ttGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAg                                                                <  2:1067614/140‑1 (MQ=255)

                                                                       ttGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAg                                                                <  1:365872/140‑1 (MQ=255)

                                                                       ttGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAg                                                                <  1:331917/140‑1 (MQ=255)

                                                                       ttGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAg                                                                <  1:1504375/140‑1 (MQ=255)

                                                                       ttGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAg                                                                >  2:365872/1‑140 (MQ=255)

                                                                       ttGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAg                                                                <  1:1173926/140‑1 (MQ=255)

                                                                       ttGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGCCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAg                                                                >  2:144720/1‑140 (MQ=255)

                                                                        tGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCa                                                                         >  2:917193/1‑130 (MQ=255)

                                                                        tGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCa                                                                         <  1:917193/130‑1 (MQ=255)

                                                                           tGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGAt                                                            >  1:666020/1‑140 (MQ=255)

                                                                           tGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGAt                                                            >  1:1479283/1‑140 (MQ=255)

                                                                           tGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGAt                                                            >  1:342702/1‑140 (MQ=255)

                                                                               gCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGa                                                        >  1:656150/1‑140 (MQ=255)

                                                                                cTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAg                                                                <  1:1034727/131‑1 (MQ=255)

                                                                                cTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAg                                                                >  2:1034727/1‑131 (MQ=255)

                                                                                   tCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCt                                                    >  2:314460/1‑140 (MQ=255)

                                                                                        tATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAgccg                                               <  1:1086104/140‑1 (MQ=255)

                                                                                         aTTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAgccgc                                              >  2:1203309/1‑140 (MQ=255)

                                                                                         aTTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGCCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAgccgc                                              <  1:144720/140‑1 (MQ=255)

                                                                                          tttCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAgccgcc                                             >  1:20728/1‑140 (MQ=255)

                                                                                          tttCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAgccgcc                                             >  1:1492153/1‑140 (MQ=255)

                                                                                           ttCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCa                                            <  1:1081229/140‑1 (MQ=255)

                                                                                           ttCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCa                                            <  1:614133/140‑1 (MQ=255)

                                                                                             ctcATTTTTGTGGGTGATACCAATTTGAATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGGCGCTAGCCGCCACg                                          <  2:666020/140‑1 (MQ=255)

                                                                                             ctcATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAgccgc                                              >  2:1492103/1‑136 (MQ=255)

                                                                                             ctcATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAgccgc                                              <  1:1492103/136‑1 (MQ=255)

                                                                                             ctcATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACg                                          <  1:64531/140‑1 (MQ=255)

                                                                                             ctcATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACg                                          >  1:1263789/1‑140 (MQ=255)

                                                                                               cATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAgccgc                                              <  1:231647/134‑1 (MQ=255)

                                                                                               cATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAgccgc                                              >  2:231647/1‑134 (MQ=255)

                                                                                                aTTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTg                                       >  1:604569/1‑140 (MQ=255)

                                                                                                 tttttGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGa                                      >  1:1250849/1‑140 (MQ=255)

                                                                                                    ttGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGaaaa                                   <  2:54409/140‑1 (MQ=255)

                                                                                                    ttGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGaaaa                                   >  2:384587/1‑140 (MQ=255)

                                                                                                      gTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAac                                 <  2:1263789/140‑1 (MQ=255)

                                                                                                      gTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAac                                 <  1:1228058/140‑1 (MQ=255)

                                                                                                         gatgatACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACAcc                              <  2:1250849/140‑1 (MQ=255)

                                                                                                         gatgatACCAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACAcc                              <  2:69199/140‑1 (MQ=255)

                                                                                                                 cAATTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGAAAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAacac                      <  2:768839/140‑1 (MQ=255)

                                                                                                                   tttttGATGGTGTCGTTTGCCGCGG‑‑GGGGGTTTTGTGCTGGCCTCCCATCGGATGTTGCTACAGGATATCTTCGAAAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACAccg                    <  2:1522716/139‑1 (MQ=255)

                                                                                                                   aTTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACAccg                    <  1:1332856/140‑1 (MQ=255)

                                                                                                                   aTTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACAccg                    <  1:1487513/140‑1 (MQ=255)

                                                                                                                   aTTTTGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACAccg                    <  2:1492153/140‑1 (MQ=255)

                                                                                                                    ttttGATGGTGTCGTTTGCCGC‑AGGGGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACAccg                    <  1:1338049/140‑1 (MQ=255)

                                                                                                                     tttGATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACAccgcc                  >  2:1210248/1‑140 (MQ=255)

                                                                                                                        gATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAacac                      >  2:306048/1‑133 (MQ=255)

                                                                                                                        gATGGTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAacac                      <  1:306048/133‑1 (MQ=255)

                                                                                                                           ggTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACACCGCCGTTcag            <  2:604569/140‑1 (MQ=255)

                                                                                                                           ggTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACACCGCCGTTcag            <  1:1066021/140‑1 (MQ=255)

                                                                                                                           ggTGTCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACACCGCCGTTcag            <  1:191874/140‑1 (MQ=255)

                                                                                                                               tCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACACCGCCGTTCagcagc        >  2:1296760/1‑140 (MQ=255)

                                                                                                                               tCGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACACCGCCGTTCagcagc        >  1:625815/1‑140 (MQ=255)

                                                                                                                                cGTTTGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACCCCATCAACACCGCCGTTCAGCAGCg       <  2:1479283/140‑1 (MQ=255)

                                                                                                                                  tttGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACACCGCCGTTCAGCAGCGTa     <  1:1382283/140‑1 (MQ=255)

                                                                                                                                  tttGCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACACCGCCGTTCAGCAGCGTa     <  1:314460/140‑1 (MQ=255)

                                                                                                                                     gCCGC‑AG‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACACCGCCGTTCAGCAGCGTACGa  <  1:1210248/140‑1 (MQ=255)

CCAACGCCAACCGGTTATTGCGCACCTGATTCGCGCGACAGAACGCTTTAACGATCGGCTGGGTAACCAGTTTGGTGCGGCTATCACCTATTTCTCATTTTTGTCGATGATACCAATTTTGATGGTGTCGTTTGCCGC‑A‑‑GGGGGCTTTGTGCTGGCCTCCCATCCGATGTTGCTACAGGATATCTTCGACAAAATTCTGCAAAACATCAGCGATCCGACGCTAGCCGCCACGTTGAAAAACACCATCAACACCGCCGTTCAGCAGCGTACGA  >  NC_000913/3673496‑3673767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A