Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	138	91.1	3071990	99.2%	3047414	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,026,686	A→G	T54A (ACG→GCG)	fre →	flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,026,686	0	A	G	100.0%	373.0 / NA	107	T54A (ACG→GCG)	fre	flavin reductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (55/52); total (55/52)

Only 100 of 107 total aligned reads displayed.

ACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCG  >  NC_000913/4026551‑4026822

aCCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGcc                                                                                                                                      >  1:1510887/1‑140 (MQ=255)

        aGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTT‑GGCGCCGGATGaaaa                                                                                                                             >  2:759691/1‑140 (MQ=255)

        aGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGCGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGaaa                                                                                                                              <  1:1106190/140‑1 (MQ=255)

         gAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGaaaa                                                                                                                             <  1:64837/140‑1 (MQ=255)

           aGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAg                                                                                                                           >  1:19901/1‑140 (MQ=255)

            gCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAgg                                                                                                                          <  2:363505/140‑1 (MQ=255)

               aTCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGtt                                                                                                                       >  2:1273272/1‑140 (MQ=255)

               aTCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGtt                                                                                                                       >  1:832963/1‑140 (MQ=255)

                 cACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTa                                                                                                                     <  2:1510887/140‑1 (MQ=255)

                  aCGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTAt                                                                                                                    <  2:487586/140‑1 (MQ=255)

                   cGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATc                                                                                                                   <  2:744354/140‑1 (MQ=255)

                    ggATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCg                                                                                                                  <  2:890385/140‑1 (MQ=255)

                    ggATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCg                                                                                                                  <  2:348382/140‑1 (MQ=255)

                     gATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGa                                                                                                                 >  1:131484/1‑140 (MQ=255)

                      aTACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAg                                                                                                                <  2:19901/140‑1 (MQ=255)

                        aCCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAg                                                                                                                >  1:1111687/1‑138 (MQ=255)

                        aCCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAg                                                                                                                <  2:1111687/138‑1 (MQ=255)

                        aCCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCt                                                                                                              >  2:1302896/1‑140 (MQ=255)

                        aCCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTT‑GGCGCCGGATGAAAAAGGGTTTATCGAGCTg                                                                                                             <  1:759691/140‑1 (MQ=255)

                          cGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGc                                                                                                            >  2:528341/1‑140 (MQ=255)

                          cGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGc                                                                                                            <  2:928800/140‑1 (MQ=255)

                              tatCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCatat                                                                                                        >  2:561017/1‑140 (MQ=255)

                                  gtgtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGgc                                                                                                    >  2:351886/1‑140 (MQ=255)

                                   tgtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGgcg                                                                                                   >  2:192743/1‑140 (MQ=255)

                                    gtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATc                                                                                                                   <  2:1355407/123‑1 (MQ=255)

                                    gtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATc                                                                                                                   >  1:1355407/1‑123 (MQ=255)

                                    gtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGgcgc                                                                                                  >  2:820574/1‑140 (MQ=255)

                                    gtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGgcgc                                                                                                  >  2:517747/1‑140 (MQ=255)

                                     tCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCt                                                                                                 >  1:42739/1‑140 (MQ=255)

                                      ccGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAA‑GGTTTATCGAGCTGCATATTGGCGCTTc                                                                                               >  2:464063/1‑140 (MQ=255)

                                          aTCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGa                                                                                            >  1:1354424/1‑140 (MQ=255)

                                               gCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTc                                                                                               >  1:830500/1‑132 (MQ=255)

                                               gCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTc                                                                                               <  2:830500/132‑1 (MQ=255)

                                                    aCGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCtt                                                                                  >  2:254619/1‑140 (MQ=255)

                                                        ggCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAcg                                                                              >  2:446409/1‑140 (MQ=255)

                                                        ggCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAcg                                                                              >  2:1442132/1‑140 (MQ=255)

                                                        ggCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAcg                                                                              <  1:517747/140‑1 (MQ=255)

                                                         gCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAcgc                                                                             >  1:1113844/1‑140 (MQ=255)

                                                         gCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAcgc                                                                             <  1:314836/140‑1 (MQ=255)

                                                         gCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAcgc                                                                             >  1:282755/1‑140 (MQ=255)

                                                         gCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAcgc                                                                             >  2:64851/1‑140 (MQ=255)

                                                              ttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAg                                                                        <  2:127429/140‑1 (MQ=255)

                                                              ttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAg                                                                        <  2:1529024/140‑1 (MQ=255)

                                                              ttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAg                                                                        <  1:971461/140‑1 (MQ=255)

                                                              ttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAg                                                                        <  1:1442132/140‑1 (MQ=255)

                                                              ttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAg                                                                        <  2:282755/140‑1 (MQ=255)

                                                              ttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAg                                                                        <  2:306873/140‑1 (MQ=255)

                                                              ttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAg                                                                        <  2:875152/140‑1 (MQ=255)

                                                                    tcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCa                                                                  >  1:479124/1‑140 (MQ=255)

                                                                    tcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCa                                                                  >  2:1403345/1‑140 (MQ=255)

                                                                         cTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGAc                                                             <  1:192743/140‑1 (MQ=255)

                                                                           ggTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCg                                                           <  1:605271/140‑1 (MQ=255)

                                                                           ggTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCg                                                           <  1:528341/140‑1 (MQ=255)

                                                                            gTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGc                                                          <  1:1302896/140‑1 (MQ=255)

                                                                            gTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGc                                                          <  1:69698/140‑1 (MQ=255)

                                                                            gTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAA‑GGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCa                                                         <  1:464063/140‑1 (MQ=255)

                                                                             tCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCa                                                         <  1:561017/140‑1 (MQ=255)

                                                                             tCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCa                                                         <  2:74074/140‑1 (MQ=255)

                                                                              cAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCAt                                                        <  1:351886/140‑1 (MQ=255)

                                                                                     tGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCaaa                                                 <  2:1354424/140‑1 (MQ=255)

                                                                                      gATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAg                                                >  2:840218/1‑140 (MQ=255)

                                                                                          gTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGatca                                            <  2:479124/140‑1 (MQ=255)

                                                                                           tAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGa                                               >  2:788257/1‑136 (MQ=255)

                                                                                           tAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGa                                               <  1:788257/136‑1 (MQ=255)

                                                                                             gTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGAtcatca                                         >  2:628819/1‑140 (MQ=255)

                                                                                             gTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGAtcatca                                         >  1:1289933/1‑140 (MQ=255)

                                                                                             gTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGAtcatca                                         >  2:300126/1‑140 (MQ=255)

                                                                                              tGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCaa                                        >  2:1200470/1‑140 (MQ=255)

                                                                                               gatggataAGCGCGACAAACGTCCGTTCTAAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCaaa                                       <  2:223967/140‑1 (MQ=255)

                                                                                                   gatgAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGt                                   >  2:101254/1‑140 (MQ=255)

                                                                                                     tgAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTgg                                 >  2:548463/1‑140 (MQ=255)

                                                                                                     tgAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTgg                                 >  2:313044/1‑140 (MQ=255)

                                                                                                     tgAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTgg                                 <  2:42739/140‑1 (MQ=255)

                                                                                                       aGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTc                               >  2:1008530/1‑140 (MQ=255)

                                                                                                        gcgcGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCg                              >  2:313647/1‑140 (MQ=255)

                                                                                                        gcgcGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCg                              <  1:64851/140‑1 (MQ=255)

                                                                                                        gcgcGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCg                              >  2:1123573/1‑140 (MQ=255)

                                                                                                         cgcgACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGa                             >  2:401725/1‑140 (MQ=255)

                                                                                                          gcgACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGAc                            >  1:1007466/1‑140 (MQ=255)

                                                                                                            gACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACAt                          >  2:838595/1‑140 (MQ=255)

                                                                                                            gACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACAt                          <  1:401725/140‑1 (MQ=255)

                                                                                                             aCAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAGGATCATCAAATCGTGGTCGACAtt                         >  2:940419/1‑140 (MQ=255)

                                                                                                             aCAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACAtt                         <  2:1259016/140‑1 (MQ=255)

                                                                                                             aCAAACGTCCGTTCTCAATGGCCTCGGCGCCGGATGAAAAAGGGTTTATCGAGCCGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACAtt                         >  1:1217729/1‑140 (MQ=255)

                                                                                                               aaaCGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTcc                       <  2:23328/140‑1 (MQ=255)

                                                                                                                 aCGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTcccc                     <  1:1008530/140‑1 (MQ=255)

                                                                                                                 aCGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTcccc                     <  1:548463/140‑1 (MQ=255)

                                                                                                                     ccGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACgg                 <  1:254619/140‑1 (MQ=255)

                                                                                                                        ttCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGgaga              >  1:1089789/1‑140 (MQ=255)

                                                                                                                        ttCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGgaga              >  2:413566/1‑140 (MQ=255)

                                                                                                                            cAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCg          <  1:1365067/140‑1 (MQ=255)

                                                                                                                             aaTGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGAGt         >  2:1452216/1‑140 (MQ=255)

                                                                                                                               tGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTgg       >  1:1382575/1‑140 (MQ=255)

                                                                                                                                  cTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTg    >  2:102747/1‑140 (MQ=255)

                                                                                                                                  cTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTg    >  1:726542/1‑140 (MQ=255)

                                                                                                                                  cTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTg    <  1:69852/140‑1 (MQ=255)

                                                                                                                                  cTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTg    >  1:1409286/1‑140 (MQ=255)

                                                                                                                                    tCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTgcg  <  2:1289933/140‑1 (MQ=255)

                                                                                                                                    tCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTgcg  <  1:313647/140‑1 (MQ=255)

                                                                                                                                    tCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTgcg  <  1:102747/140‑1 (MQ=255)

ACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCG  >  NC_000913/4026551‑4026822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A