Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	138	91.1	3071990	99.2%	3047414	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,320,364	A→G	R100R (CGT→CGC)	phnI ←	carbon‑phosphorus lyase core complex subunit PhnI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,320,364	0	A	G	100.0%	162.7 / NA	48	R100R (CGT→CGC)	phnI	carbon‑phosphorus lyase core complex subunit PhnI
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (30/18); total (30/18)

TCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCG  >  NC_000913/4320227‑4320499

tCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCg                                                                                                                                       >  1:855296/1‑140 (MQ=255)

   gcggTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCAt                                                                                                                                    <  2:508791/140‑1 (MQ=255)

   gcggTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCAt                                                                                                                                    <  2:209680/140‑1 (MQ=255)

          tCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCggtg                                                                                                                             <  1:250481/140‑1 (MQ=255)

                  ggCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAg                                                                                                                     <  1:666228/140‑1 (MQ=255)

                   gcgcTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAACAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGc                                                                                                                    >  2:565759/1‑140 (MQ=255)

                    cgcTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCTCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCg                                                                                                                   <  2:1427234/140‑1 (MQ=255)

                       ttCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCt                                                                                                                <  2:855296/140‑1 (MQ=255)

                          gCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAACAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGc                                                                                                             <  1:565759/140‑1 (MQ=255)

                                         ggTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCg                                                                                              >  1:1431617/1‑140 (MQ=255)

                                         ggTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCg                                                                                              >  1:293135/1‑140 (MQ=255)

                                          gTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGc                                                                                             >  2:88922/1‑140 (MQ=255)

                                            aaaaTCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCa                                                                                           <  2:1431617/140‑1 (MQ=255)

                                                tCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGTCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACgt                                                                                       <  2:828886/140‑1 (MQ=255)

                                                   aGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACgtggt                                                                                    >  2:949402/1‑140 (MQ=255)

                                                    gCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACgtggtg                                                                                   >  1:291743/1‑140 (MQ=255)

                                                    gCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACgtggtg                                                                                   >  1:886981/1‑140 (MQ=255)

                                                                  tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGCGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCgcag                                                                     <  2:293135/140‑1 (MQ=255)

                                                                  tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGgcg                                                                          <  1:400449/135‑1 (MQ=255)

                                                                  tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGgcg                                                                          >  2:1121967/1‑135 (MQ=255)

                                                                  tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGgcg                                                                          <  1:1121967/135‑1 (MQ=255)

                                                                  tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGgcg                                                                          >  2:400449/1‑135 (MQ=255)

                                                                  tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCgcag                                                                     >  1:721784/1‑140 (MQ=255)

                                                                  tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCgcag                                                                     >  1:72907/1‑140 (MQ=255)

                                                                        tAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGaa                                                               >  1:237640/1‑140 (MQ=255)

                                                                        tAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGaa                                                               >  1:287569/1‑140 (MQ=255)

                                                                         aGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGaaa                                                              >  1:666907/1‑140 (MQ=255)

                                                                               ggCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCt                                                        >  2:1204412/1‑140 (MQ=255)

                                                                                 ccAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTc                                                      <  1:949402/140‑1 (MQ=255)

                                                                                     gcagcTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACg                                                  >  1:431428/1‑140 (MQ=255)

                                                                                         cTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTAt                                              >  1:3628/1‑140 (MQ=255)

                                                                                         cTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTAt                                              <  1:88922/140‑1 (MQ=255)

                                                                                         cTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTAt                                              <  1:1204412/140‑1 (MQ=255)

                                                                                              cgccgGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTc                                                      >  2:364645/1‑127 (MQ=255)

                                                                                              cgccgGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTc                                                      <  1:364645/127‑1 (MQ=255)

                                                                                                           tttATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTca                            >  2:1312057/1‑140 (MQ=255)

                                                                                                                  aCGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccag                     >  1:238848/1‑140 (MQ=255)

                                                                                                                   cggcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccagc                    >  1:1437994/1‑140 (MQ=255)

                                                                                                                   cggcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccagc                    >  1:1411043/1‑140 (MQ=255)

                                                                                                                   cggcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgcc                       <  1:1260648/137‑1 (MQ=255)

                                                                                                                   cggcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcg                         <  1:109256/135‑1 (MQ=255)

                                                                                                                   cggcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcg                         >  2:109256/1‑135 (MQ=255)

                                                                                                                   cggcggAGATACGGCGTTCGAGGCGCATCCCGGGGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgcc                       >  2:1260648/1‑137 (MQ=255)

                                                                                                                     gcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccagcgc                  >  2:482510/1‑140 (MQ=255)

                                                                                                                     gcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccagcgc                  >  1:1218288/1‑140 (MQ=255)

                                                                                                                     gcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccagcgc                  >  2:935651/1‑140 (MQ=255)

                                                                                                                     gcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTAGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgctagcgc                  >  2:1356385/1‑140 (MQ=255)

                                                                                                                            tACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGt           <  1:1356385/140‑1 (MQ=255)

                                                                                                                                   ttCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGt    >  1:804293/1‑140 (MQ=255)

                                                                                                                                     cGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCg  <  2:1437994/140‑1 (MQ=255)

TCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCG  >  NC_000913/4320227‑4320499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A