Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	138	91.1	3071990	99.2%	3047414	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,451,387	A→G	E110E (GAA→GAG)	ytfR →	galactofuranose ABC transporter putative ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,451,387	0	A	G	100.0%	192.5 / NA	55	E110E (GAA→GAG)	ytfR	galactofuranose ABC transporter putative ATP binding subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/26); total (29/26)

ACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTC  >  NC_000913/4451250‑4451519

aCCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGcc                                                                                                                                    >  1:1331713/1‑140 (MQ=255)

  cATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCa                                                                                                                                  >  2:44970/1‑140 (MQ=255)

   aTCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCaa                                                                                                                                 <  1:151100/140‑1 (MQ=255)

    tCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCaaa                                                                                                                                >  2:1338046/1‑140 (MQ=255)

    tCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCaaa                                                                                                                                >  1:90709/1‑140 (MQ=255)

       ggctggAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGc                                                                                                                             >  2:1342949/1‑140 (MQ=255)

         ctggAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCtt                                                                                                                           <  2:989493/140‑1 (MQ=255)

           ggAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCACCCTGCTACCCAATATGTCTGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCg                                                                                                                         <  2:1366164/140‑1 (MQ=255)

           ggAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCg                                                                                                                         <  1:686647/140‑1 (MQ=255)

              aGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGcc                                                                                                                      >  1:802310/1‑140 (MQ=255)

                  cAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCa                                                                                                                                  >  2:529436/1‑124 (MQ=255)

                  cAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCa                                                                                                                                  <  1:529436/124‑1 (MQ=255)

                  cAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCt                                                                                                                  >  1:746826/1‑140 (MQ=255)

                      cTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTAcgc                                                                                                              <  1:1342949/140‑1 (MQ=255)

                          ctcACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCa                                                                                                          <  1:1338046/140‑1 (MQ=255)

                           tcACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCaa                                                                                                         <  2:799821/140‑1 (MQ=255)

                             aCCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAag                                                                                                       <  2:802310/140‑1 (MQ=255)

                               cGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAagag                                                                                                     >  1:593458/1‑140 (MQ=255)

                                     aTACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGaa                                                                                               <  2:849422/140‑1 (MQ=255)

                                          gCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAgcg                                                                                          <  1:1431739/140‑1 (MQ=255)

                                                gcgcAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCAc                                                                                    >  1:1257915/1‑140 (MQ=255)

                                                    aacaacTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGaa                                                                                <  1:1325996/140‑1 (MQ=255)

                                                        acTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGa                                                                            <  2:1282934/140‑1 (MQ=255)

                                                          tCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATg                                                                          <  2:608533/140‑1 (MQ=255)

                                                          tCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATg                                                                          <  2:1257915/140‑1 (MQ=255)

                                                          tCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATg                                                                          <  1:1342068/140‑1 (MQ=255)

                                                                 tGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCtt                                                                   <  1:150551/140‑1 (MQ=255)

                                                                  ggCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTa                                                                  <  2:1331713/140‑1 (MQ=255)

                                                                      ccGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGt                                                              <  2:746826/140‑1 (MQ=255)

                                                                        gTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGttt                                                            >  2:465193/1‑140 (MQ=255)

                                                                          cTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTtct                                                          <  2:90709/140‑1 (MQ=255)

                                                                             tCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTccc                                                       >  1:1199256/1‑140 (MQ=255)

                                                                                   aGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACg                                                 >  1:829715/1‑140 (MQ=255)

                                                                                    gTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGt                                                >  1:1159910/1‑140 (MQ=255)

                                                                                      cAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTgc                                              <  2:593458/140‑1 (MQ=255)

                                                                                         ccTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGcgcg                                           <  1:912532/140‑1 (MQ=255)

                                                                                               aCCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGc                                     >  2:1128308/1‑140 (MQ=255)

                                                                                                   aaTATGTCGTTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACCGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCaa                                 >  1:1280739/1‑140 (MQ=255)

                                                                                                     tatGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAAcc                               >  1:1458257/1‑140 (MQ=255)

                                                                                                          cggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCttt                          >  2:778275/1‑140 (MQ=255)

                                                                                                          cggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCttt                          >  1:1354401/1‑140 (MQ=255)

                                                                                                          cggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCttt                          >  2:511684/1‑140 (MQ=255)

                                                                                                           ggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGaa                                         <  2:1394766/124‑1 (MQ=255)

                                                                                                           ggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGaa                                         >  1:1394766/1‑124 (MQ=255)

                                                                                                           ggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCtttt                         >  1:867347/1‑140 (MQ=255)

                                                                                                           ggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCtttt                         >  1:1528118/1‑140 (MQ=255)

                                                                                                               gCTGATAATCTATTTATAGGCCGCGAGCCCGAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGt                     >  1:852763/1‑140 (MQ=255)

                                                                                                               gCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGt                     >  1:1236545/1‑140 (MQ=255)

                                                                                                                          aTTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATgcagc          >  1:268943/1‑140 (MQ=255)

                                                                                                                                 ggCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAAtcgt   <  2:867347/140‑1 (MQ=255)

                                                                                                                                  gCCGCTAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAAtcgtc  >  1:794427/1‑140 (MQ=255)

                                                                                                                                  gCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTcg                   <  2:239765/123‑1 (MQ=255)

                                                                                                                                  gCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTcg                   >  1:239765/1‑123 (MQ=255)

                                                                                                                                     gcgAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAAtcgt   <  2:1166584/136‑1 (MQ=255)

                                                                                                                                     gcgAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAAtcgt   >  1:1166584/1‑136 (MQ=255)

ACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTC  >  NC_000913/4451250‑4451519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A