Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 172 67.8 2301510 98.9% 2276193 138.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,409,679 C→T N135N (AAC→AAT)  rlmB → 23S rRNA 2'‑O‑ribose G2251 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,409,6790CT97.9% 328.9 / ‑3.1 96N135N (AAC→AAT) rlmB23S rRNA 2'‑O‑ribose G2251 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (44/50);  total (44/52)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATA  >  NC_000913/4409543‑4409815
                                                                                                                                        |                                                                                                                                        
cttcgctCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCt                                                                                                                                       <  2:618783/140‑1 (MQ=255)
 ttcgctCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTa                                                                                                                                      >  1:862314/1‑140 (MQ=255)
             aCCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAg                                                                                                                          >  1:50132/1‑140 (MQ=255)
                   ccTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCt                                                                                                                    >  1:1033871/1‑140 (MQ=255)
                   ccTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCt                                                                                                                    >  2:1061780/1‑140 (MQ=255)
                    ctgctgATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTg                                                                                                                   >  2:819510/1‑140 (MQ=255)
                    ctgctgATCCTCGACGGAGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTg                                                                                                                   >  2:240253/1‑140 (MQ=255)
                      gctgATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTgcg                                                                                                                 >  1:771435/1‑140 (MQ=255)
                      gctgATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTgcg                                                                                                                 >  1:261688/1‑140 (MQ=255)
                         gATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTgcggcg                                                                                                              >  1:157555/1‑140 (MQ=255)
                         gATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTgcggcg                                                                                                              <  1:819510/140‑1 (MQ=255)
                            ccTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTgcggcgcgg                                                                                                           >  1:825452/1‑140 (MQ=255)
                            ccTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTgcggcgcgg                                                                                                           >  2:195576/1‑140 (MQ=255)
                             cTCGACGGAGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTgcggcgcggc                                                                                                          <  1:240253/140‑1 (MQ=255)
                                 aCGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGaa                                                                                                      >  2:463569/1‑140 (MQ=255)
                                    gCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGc                                                                                                   >  1:948076/1‑140 (MQ=255)
                                     cGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCg                                                                                                  >  2:265934/1‑140 (MQ=255)
                                     cGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCg                                                                                                  <  2:862314/140‑1 (MQ=255)
                                      gTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGt                                                                                                 >  1:608785/1‑140 (MQ=255)
                                          ccGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCg                                                                                             <  2:50132/140‑1 (MQ=255)
                                                   acaACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCgg                                                                                    <  2:771435/140‑1 (MQ=255)
                                                   acaACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCgg                                                                                    <  1:1061780/140‑1 (MQ=255)
                                                   acaACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTCTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCgg                                                                                    <  1:994549/140‑1 (MQ=255)
                                                       ccTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGa                                                                                >  1:1148257/1‑140 (MQ=255)
                                                        cTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGAc                                                                               >  1:88235/1‑140 (MQ=255)
                                                          cGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTa                                                                             >  2:215385/1‑140 (MQ=255)
                                                           ggCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTaa                                                                            >  1:1043049/1‑140 (MQ=255)
                                                             cgcgTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAAcc                                                                          <  2:1043049/140‑1 (MQ=255)
                                                                 tGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGGTTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTgc                                                                      >  1:936648/1‑140 (MQ=255)
                                                                 tGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTgc                                                                      >  2:345970/1‑140 (MQ=255)
                                                                 tGCCTGCGTAGTCCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATGGCTCCGCCCAGCTCAATGCTACGGCGAAAAAAGAACCCCGGGGCCCGGCAGAAAGCGTTCCGCTGATTCCGGCGACTAACCTTgc                                                                      >  2:907494/1‑140 (MQ=255)
                                                                  gCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAGGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTgcg                                                                     <  2:11666/140‑1 (MQ=255)
                                                                  gCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTgcg                                                                     <  1:359622/140‑1 (MQ=255)
                                                                  gCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTgcg                                                                     <  1:345970/140‑1 (MQ=255)
                                                                  gCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTgcg                                                                     <  2:88235/140‑1 (MQ=255)
                                                                    cTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTgcgcg                                                                   <  2:1148257/140‑1 (MQ=255)
                                                                       cGTAGTTCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCAc                                                                <  1:121494/140‑1 (MQ=255)
                                                                       cGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCAc                                                                <  2:1137943/140‑1 (MQ=255)
                                                                        gTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCAcc                                                               <  1:971724/140‑1 (MQ=255)
                                                                           gTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATg                                                            <  2:261688/140‑1 (MQ=255)
                                                                            tGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCGACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGc                                                           <  2:1006696/140‑1 (MQ=255)
                                                                            tGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTgcg                                                                     >  1:681950/1‑130 (MQ=255)
                                                                            tGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTgcg                                                                     <  2:681950/130‑1 (MQ=255)
                                                                            cgCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCa                                                                 >  1:643989/2‑134 (MQ=255)
                                                                            cgCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCa                                                                 <  2:643989/133‑1 (MQ=255)
                                                                                 aCGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCa                                                                 <  2:510299/129‑1 (MQ=255)
                                                                                 aCGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCa                                                                 >  1:510299/1‑129 (MQ=255)
                                                                                  cgccgcAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGt                                                     >  2:315632/1‑140 (MQ=255)
                                                                                     cgcAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGc                                                  >  2:1048630/1‑140 (MQ=255)
                                                                                            gTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAaga                                           <  2:608785/140‑1 (MQ=255)
                                                                                            gTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAaga                                           <  1:215385/140‑1 (MQ=255)
                                                                                             ttCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGGGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAagag                                          <  1:668437/140‑1 (MQ=255)
                                                                                             ttCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAagag                                          <  2:1033871/140‑1 (MQ=255)
                                                                                             ttCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAagag                                          <  1:265934/140‑1 (MQ=255)
                                                                                                    ggTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCt                                   >  1:647425/1‑140 (MQ=255)
                                                                                                    ggTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCt                                   >  1:523824/1‑140 (MQ=255)
                                                                                                        aTTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAagag                                          <  2:856477/129‑1 (MQ=255)
                                                                                                        aTTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAagag                                          >  1:856477/1‑129 (MQ=255)
                                                                                                        aTTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAagag                                          <  1:541702/129‑1 (MQ=255)
                                                                                                        aTTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAagag                                          >  2:541702/1‑129 (MQ=255)
                                                                                                        aTTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGAt                               >  1:586993/1‑140 (MQ=255)
                                                                                                        aTTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGAt                               >  2:275598/1‑140 (MQ=255)
                                                                                                        aTTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGAt                               >  1:417316/1‑140 (MQ=255)
                                                                                                        aTTGTGCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGAt                               >  1:1057864/1‑140 (MQ=255)
                                                                                                            tgCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGAtcgtc                           <  2:936648/140‑1 (MQ=255)
                                                                                                            tgCCGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGAtcgtc                           >  1:604713/1‑140 (MQ=255)
                                                                                                              ccGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAaga                                           <  2:468332/122‑1 (MQ=255)
                                                                                                              ccGAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAaga                                           >  1:468332/1‑122 (MQ=255)
                                                                                                                gAAAGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTa                       >  1:729153/1‑140 (MQ=255)
                                                                                                                  aaGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACg                     >  1:767723/1‑140 (MQ=255)
                                                                                                                   aGATCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACgg                    >  2:895836/1‑140 (MQ=255)
                                                                                                                     aTCGCTCCGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCa                  <  2:729153/140‑1 (MQ=255)
                                                                                                                           ccGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCaggcgag            <  1:315632/140‑1 (MQ=255)
                                                                                                                            cGCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCaggcgagg           >  1:930817/1‑140 (MQ=255)
                                                                                                                             gCACCGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCaggcgaggc          <  2:1057864/140‑1 (MQ=255)
                                                                                                                             gCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCaggcgaggc          <  2:371852/140‑1 (MQ=255)
                                                                                                                             gCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCaggcgaggc          <  1:275598/140‑1 (MQ=255)
                                                                                                                             gCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCaggcgaggc          <  1:391723/140‑1 (MQ=255)
                                                                                                                             gCACAGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCaggcgaggc          <  2:604713/140‑1 (MQ=255)
                                                                                                                              cacaGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCaggcgaggcg         <  2:767723/140‑1 (MQ=255)
                                                                                                                              cacaGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCaggcgaggcg         <  2:647425/140‑1 (MQ=255)
                                                                                                                              cacaGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCaggcgaggcg         <  2:523824/140‑1 (MQ=255)
                                                                                                                              cacaGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCaggcgaggcg         <  2:157555/140‑1 (MQ=255)
                                                                                                                               acaGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCgg        <  2:948076/140‑1 (MQ=255)
                                                                                                                               acaGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCgg        <  1:453588/140‑1 (MQ=255)
                                                                                                                               acaGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCgg        <  1:195576/140‑1 (MQ=255)
                                                                                                                               acaGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCgg        <  1:1048630/140‑1 (MQ=255)
                                                                                                                                 aGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACgg                    >  1:1034280/1‑126 (MQ=255)
                                                                                                                                 aGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACgg                    <  2:1034280/126‑1 (MQ=255)
                                                                                                                                 aGCTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGAt      >  1:94929/1‑140 (MQ=255)
                                                                                                                                   cTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCa    <  2:586993/140‑1 (MQ=255)
                                                                                                                                   cTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCa    >  1:378917/1‑140 (MQ=255)
                                                                                                                                   cTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGACAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCa    <  2:76092/140‑1 (MQ=255)
                                                                                                                                   cTCAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGACAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCa    >  1:76092/1‑140 (MQ=255)
                                                                                                                                     cAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATa  <  2:378917/140‑1 (MQ=255)
                                                                                                                                     cAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATa  <  1:895836/140‑1 (MQ=255)
                                                                                                                                     cAATGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATa  <  1:619487/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
CTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATA  >  NC_000913/4409543‑4409815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A