breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 11,741 T→C M16V (ATG→GTG)  yaaI ← DUF2541 domain‑containing protein YaaI
RA 37,682 T→C N48S (AAC→AGC)  caiC ← carnitine‑CoA ligase
RA 52,315 T→G Q39P (CAG→CCG)  rsmA ← 16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase
RA 77,011 C→T V97I (GTT→ATT)  sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 77,593 (T)8→7 noncoding (227/227 nt) sgrS → small regulatory RNA SgrS
RA 144,096 Δ2 bp coding (395‑396/771 nt) yadH → putative ABC transporter membrane subunit YadH
RA 147,572 A→G Y202C (TAC→TGC)  rpnC → recombination‑promoting nuclease RpnC
RA 150,470 A→G S162P (TCC→CCC)  yadC ← fimbrial tip‑adhesin YadC
RA 175,956 C→T H284Y (CAC→TAC)  clcA → chloride:H(+) antiporter ClcA
RA 209,624 (T)6→5 intergenic (+44/‑55) accA → / → ldcC acetyl‑CoA carboxyltransferase subunit alpha/lysine decarboxylase 2
RA 241,310 T→C N665S (AAC→AGC)  fadE ← acyl‑CoA dehydrogenase
RA 255,174 (C)6→7 coding (543/1458 nt) pepD ← peptidase D
RA 255,984 A→G E3G (GAA→GGA)  gpt → xanthine‑guanine phsophoribosyltransferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 268,395 A→G V189A (GTC→GCC)  ykfA ← CP4‑6 prophage; putative GTP‑binding protein YkfA
RA 301,581 A→G L103L (TTA→CTA)  paoB ← aldehyde dehydrogenase, FAD‑binding subunit
RA 308,054 (C)7→8 coding (1279/2526 nt) ecpC ← putative fimbrial usher protein EcpC
RA 319,878 T→C T42A (ACC→GCC)  rclA ← putative pyridine nucleotide‑disulfide oxidoreductase RclA
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 429,137 G→A L497L (TTG→TTA secD → Sec translocon accessory complex subunit SecD
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 533,863 (T)7→6 intergenic (+37/‑53) allR → / → gcl DNA‑binding transcriptional repressor AllR/glyoxylate carboligase
RA 548,727 A→G Y124C (TAC→TGC)  ylbE → DUF1116 domain‑containing protein YlbE
RA 566,528 A→G intergenic (‑167/‑156) exoD ← / → renD DLP12 prophage; putative exonuclease ExoD/DLP12 prophage; protein RenD
RA 570,520 T→C intergenic (+92/‑373) ybcK → / → ybcL DLP12 prophage; putative recombinase/DLP12 prophage; periplasmic protein YbcL
RA 727,514 (T)7→6 coding (1219/1674 nt) kdpA ← K(+) transporting P‑type ATPase subunit KdpA
RA 817,449 A→G T136A (ACC→GCC)  moaA → GTP 3',8'‑cyclase
RA 844,562 T→G T307P (ACC→CCC)  ybiO ← moderate conductance mechanosensitive channel
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 1,009,887 (A)6→7 coding (2044/2109 nt) rlmL → fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,018,976 (A)8→9 intergenic (+39/+37) matP → / ← ompA macrodomain Ter protein/outer membrane porin A
RA 1,103,745 (T)7→8 intergenic (‑549/‑206) csgD ← / → csgB DNA‑binding transcriptional dual regulator CsgD/curlin, minor subunit
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,180,225 A→G T236A (ACC→GCC)  cobB → protein‑lysine deacetylase/desuccinylase
RA 1,219,192 T→C D70D (GAT→GAC ymgF → inner membrane protein that interacts with cell division proteins
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,223,984 (A)7→6 intergenic (+77/+295) ycgI → / ← minE protein YcgI/Z‑ring positioning protein MinE
RA 1,224,024 A→G intergenic (+117/+255) ycgI → / ← minE protein YcgI/Z‑ring positioning protein MinE
RA 1,243,818 (T)5→4 coding (710/735 nt) ycgR ← flagellar brake protein YcgR
RA 1,249,084 T→C S11G (AGC→GGC)  dhaM ← dihydroxyacetone kinase subunit M
RA 1,362,294 T→C G128G (GGT→GGC puuR → DNA‑binding transcriptional repressor PuuR
RA 1,368,615 A→G E179E (GAA→GAG pspA → phage shock protein A
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,572,854 A→G A783A (GCT→GCC pqqL ← putative zinc peptidase
RA 1,594,132 (C)8→7 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,632,949 C→T A20A (GCC→GCT ynfT → Qin prophage; protein YnfT
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,646,951 A→G intergenic (+214/+223) flxA → / ← ydfW Qin prophage; protein FlxA/Qin prophage; uncharacterized protein YdfW
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,698,891 (A)7→8 coding (290/1029 nt) malI ← DNA‑binding transcriptional repressor MalI
RA 1,701,088 (C)5→4 coding (132/1173 nt) malY → negative regulator of MalT activity/cystathionine beta‑lyase
RA 1,724,478 (G)6→8 coding (178/522 nt) sodC ← superoxide dismutase (Cu‑Zn)
RA 1,726,120 A→G E33G (GAA→GGA)  nemR → DNA‑binding transcriptional repressor NemR
RA 1,776,534 C→T G316G (GGC→GGT ydiF → putative acetate‑CoA transferase
RA 1,777,381 T→C G62G (GGT→GGC ydiO → putative acyl‑CoA dehydrogenase
RA 1,781,767 T→C F125L (TTT→CTT)  ydiS → putative oxidoreductase with FAD/NAD(P)‑binding domain
RA 1,787,398 (T)5→4 intergenic (‑286/‑47) ppsA ← / → ppsR phosphoenolpyruvate synthetase/posphoenolpyruvate synthetase regulatory protein
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,949,479 A→C G348G (GGT→GGG aspS ← aspartate‑‑tRNA ligase
RA 1,951,452 A→G T20A (ACC→GCC)  yecE → DUF72 domain‑containing protein YecE
RA 1,973,522 A→G G601G (GGT→GGC cheA ← chemotaxis protein CheA
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,037,239 A→G V305A (GTG→GCG)  hprS ← sensory histidine kinase HprS
RA 2,135,135 (C)5→4 coding (518/2163 nt) wzc ← protein‑tyrosine kinase Wzc
RA 2,135,776 T→C D108G (GAT→GGT)  wzb ← protein‑tyrosine phosphatase
RA 2,142,983 A→G intergenic (‑35/‑283) udk ← / → dgcE uridine/cytidine kinase/putative diguanylate cyclase DgcE
RA 2,153,470 T→C intergenic (+16/‑176) sibA → / → sibB small RNA SibA/small RNA SibB
RA 2,167,184 A→G noncoding (71/87 nt) cyaR → small regulatory RNA CyaR
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,253,976 (T)5→4 coding (170/1251 nt) nupX ← putative nucleoside transporter
RA 2,264,383 T→C V174A (GTA→GCA)  setB → sugar exporter SetB
RA 2,276,396 G→A E33K (GAG→AAG)  yejF → putative oligopeptide ABC transporter ATP binding subunit
RA 2,356,477 G→A G319G (GGG→GGA glpC → anaerobic glycerol‑3‑phosphate dehydrogenase subunit C
RA 2,390,123 T→C A461A (GCA→GCG nuoN ← NADH:quinone oxidoreductase subunit N
RA 2,394,435 T→C A204A (GCA→GCG nuoL ← NADH:quinone oxidoreductase subunit L
RA 2,397,887 T→C N760S (AAC→AGC)  nuoG ← NADH:quinone oxidoreductase subunit G
RA 2,408,087 A→C N176T (AAC→ACC)  alaA → glutamate‑‑pyruvate aminotransferase AlaA
RA 2,413,200 T→C intergenic (‑68/‑270) yfbV ← / → ackA UPF0208 membrane protein YfbV/acetate kinase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,531,596 (G)6→5 coding (134/762 nt) cysZ → sulfate:H(+) symporter
RA 2,554,925 A→G R86R (CGT→CGC eutR ← putative AraC‑type transcriptional regulator EutR
RA 2,588,160 A→C D189A (GAC→GCC)  acrD → multidrug efflux pump RND permease AcrD
RA 2,631,977 T→C T187A (ACC→GCC)  guaA ← GMP synthetase
RA 2,638,026 (C)6→7 coding (627/1179 nt) bamB ← outer membrane protein assembly factor BamB
RA 2,712,729 T→C G12G (GGA→GGG yfiC ← tRNA m(6)A37 methyltransferase
RA 2,764,549 T→C D202G (GAC→GGC)  abpA ← CP4‑57 prophage; anti‑bacteriophage protein
RA 2,765,794 (C)7→6 intergenic (‑18/‑124) yfjM ← / → rnlA CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system
RA 2,789,174 C→T R64C (CGT→TGT)  csiD → PF08943 family protein CsiD
RA 2,797,768 (C)5→4 coding (249/525 nt) ygaP → thiosulfate sulfurtransferase YgaP
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,838,312 G→A P318L (CCG→CTG)  ascG ← DNA‑binding transcriptional repressor AscG
RA 2,840,934 T→C Y471H (TAT→CAT)  ascF → beta‑glucoside specific PTS enzyme IIBC component
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,873,680 T→C T105A (ACC→GCC)  cysC ← adenylyl‑sulfate kinase
RA 2,874,529 A→G G297G (GGT→GGC cysN ← sulfate adenylyltransferase subunit 1
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,955,097 C→T E303K (GAA→AAA)  recB ← exodeoxyribonuclease V subunit RecB
RA 2,963,922 T→C T86A (ACC→GCC)  ppdA ← conserved protein PpdA
RA 2,966,682 A→G Y585H (TAC→CAC)  ptsP ← phosphoenolpyruvate‑protein phosphotransferase PtsP
RA 2,968,519 A→G S153S (AGT→AGC rppH ← RNA pyrophosphohydrolase
RA 2,999,175 T→C L239L (CTA→CTG ygeR ← LysM domain‑containing putative peptidase lipoprotein YgeR
RA 3,017,330 T→C L424P (CTG→CCG)  ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,024,683 T→C G111G (GGT→GGC xanQ → xanthine:H(+) symporter XanQ
RA 3,034,250 T→C T309A (ACG→GCG)  lysS ← lysine‑‑tRNA ligase, constitutive
RA 3,060,597 T→C V282A (GTC→GCC)  argP → DNA‑binding transcriptional dual regulator ArgP
RA 3,061,160 T→C V104A (GTT→GCT)  scpA → methylmalonyl‑CoA mutase
RA 3,061,966 Δ1 bp coding (1117/2145 nt) scpA → methylmalonyl‑CoA mutase
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,101,039 (C)7→6 coding (585/720 nt) yggN ← putative EcfF
RA 3,110,921 G→A T69I (ACA→ATA)  yghD ← putative type II secretion system M‑type protein
RA 3,123,278 T→C E176E (GAA→GAG glcB ← malate synthase G
RA 3,137,202 T→C T441A (ACC→GCC)  gss ← fused glutathionylspermidine amidase/glutathionylspermidine synthetase
RA 3,165,556 T→C T140A (ACG→GCG)  parC ← DNA topoisomerase IV subunit A
RA 3,222,167 T→C Y234Y (TAT→TAC ebgR → DNA‑binding transcriptional repressor EbgR
RA 3,280,758 C→T pseudogene (58/402 nt) agaW → N‑acetyl‑D‑galactosamine specific PTS truncated enzyme IIC component
RA 3,283,203 C→T P21S (CCT→TCT)  kbaY → tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaY
RA 3,303,065 T→C C193R (TGT→CGT)  yhbV → putative peptidase YhbV
RA 3,303,947 T→C V167A (GTG→GCG)  yhbW → putative luciferase‑like monooxygenase
RA 3,333,582 (A)8→7 intergenic (‑131/‑128) rplU ← / → ispB 50S ribosomal subunit protein L21/all‑trans‑octaprenyl‑diphosphate synthase
RA 3,342,809 A→G K179K (AAA→AAG kdsC → 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate phosphatase KdsC
RA 3,352,439 A→G V196A (GTT→GCT)  arcB ← sensory histidine kinase ArcB
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,368,837 T→C G337G (GGT→GGC yhcG → DUF1016 domain‑containing protein YhcG
RA 3,376,330 T→C E149E (GAA→GAG sspB ← ClpXP protease specificity‑enhancing factor
RA 3,377,627 (A)5→4 intergenic (‑207/+188) sspA ← / ← rpsI stringent starvation protein A/30S ribosomal subunit protein S9
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,479,997 A→G V204A (GTA→GCA)  kefB ← K(+) : H(+) antiporter KefB
RA 3,555,953 T→C E299E (GAA→GAG rtcA ← RNA 3'‑terminal phosphate cyclase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,561,301 T→C E51G (GAG→GGG)  glpG ← rhomboid protease
RA 3,566,281 C→T A101T (GCA→ACA)  glgP ← glycogen phosphorylase
RA 3,580,766 T→C T14A (ACC→GCC)  yhhX ← putative oxidoreductase YhhX
JC JC 3,769,841 IS5 (+) +4 bp intergenic (+161/+399) yibV → / ← yibH PF15596 family protein YibV/inner membrane protein YibH
RA 3,781,150 T→C intergenic (+133/‑65) lldD → / → trmL L‑lactate dehydrogenase/tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase
RA 3,797,041 (T)8→9 coding (94/1260 nt) waaL → O‑antigen ligase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,879,860 C→T G87D (GGC→GAC)  gyrB ← DNA gyrase subunit B
RA 3,915,508 (A)8→9 intergenic (‑308/+45) glmU ← / ← atpC fused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase/ATP synthase F1 complex subunit epsilon
RA 3,920,011 T→C K131K (AAA→AAG atpH ← ATP synthase F1 complex subunit delta
JC JC 3,923,012 IS5 (+) +4 bp intergenic (‑572/+42) atpI ← / ← rsmG ATP synthase accessory factor/16S rRNA m(7)G527 methyltransferase
RA 3,970,032 (G)6→7 coding (1002/1047 nt) wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 4,001,454 A→G N10S (AAC→AGC)  corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,044,758 T→C I187T (ATT→ACT)  yihF → uncharacterized protein YihF
RA 4,086,581 T→C V189A (GTG→GCG)  fdhD → sulfurtransferase for molybdenum cofactor sulfuration
RA 4,102,693 A→G intergenic (+20/‑129) kdgT → / → yiiM 2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter/2‑amino‑6‑N‑hydroxylaminopurine resistance protein
RA 4,110,997 C→T A171T (GCA→ACA)  tpiA ← triose‑phosphate isomerase
RA 4,165,046 A→G T470A (ACC→GCC)  btuB → cobalamin/cobinamide outer membrane transporter
RA 4,247,760 A→G N326S (AAC→AGC)  malK → maltose ABC transporter ATP binding subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,307,702 (T)7→6 coding (98/930 nt) alsK ← D‑allose kinase
RA 4,321,836 C→T C196Y (TGC→TAC)  phnF ← putative transcriptional regulator PhnF
RA 4,323,166 A→G pseudogene (116/839 nt) phnE ← phosphonate ABC transporter membrane subunit
RA 4,354,227 T→C E164G (GAA→GGA)  lysU ← lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
RA 4,367,098 A→G G410G (GGT→GGC aspA ← aspartate ammonia‑lyase
RA 4,409,679 C→T N135N (AAC→AAT rlmB → 23S rRNA 2'‑O‑ribose G2251 methyltransferase
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,488,579 (C)7→8 coding (1485/1503 nt) yjgR ← DUF853 domain‑containing protein YjgR
RA 4,551,336 G→A intergenic (‑40/‑300) gntP ← / → uxuA fructuronate transporter/D‑mannonate dehydratase
RA 4,558,205 A→G S29P (TCC→CCC)  hypT ← DNA‑binding transcriptional dual regulator HypT
RA 4,569,452 A→G pseudogene (455/921 nt) rpnD → truncated recombination‑promoting nuclease‑like protein RpnD
RA 4,577,067 (C)6→7 coding (892/1047 nt) mcrC ← 5‑methylcytosine‑specific restriction enzyme subunit McrC
RA 4,595,790 Δ1 bp coding (62/915 nt) lgoR ← putative DNA‑binding transcriptional regulator LgoR
RA 4,605,629 A→G V12A (GTC→GCC)  fhuF ← hydroxamate siderophore iron reductase
RA 4,606,094 (G)8→7 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,606,106 (C)5→6 noncoding (60/87 nt) leuV ← tRNA‑Leu
RA 4,609,336 (C)7→8 intergenic (+13/‑78) yjjG → / → prfC pyrimidine nucleotidase/peptide chain release factor RF3
RA 4,636,816 A→G T41A (ACG→GCG)  creC → sensory histidine kinase CreC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 226710–226968 228907–228689 1722–2198 20 [12] [18] 22 [rrlH]–rrfH [rrlH],rrfH
* * ÷ NC_000913 1633021–1633220 1634547–1634098 879–1527 21 [18] [16] 19 ydfK–[tfaQ] ydfK,pinQ,[tfaQ]
* * ÷ NC_000913 3423766–3424234 3424540–3424240 7–775 19 [18] [17] 19 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =86 (1.280)24 (0.400) 22/242 1.6 25.4% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 65 (1.090)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)66 (1.010) 48/266 0.3 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =99 (1.470)62 (0.930) 42/272 0.5 55.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)71 (1.060) 54/274 0.2 97.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA