Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	172	67.8	2301510	98.9%	2276193	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,605,629	A→G	V12A (GTC→GCC)	fhuF ←	hydroxamate siderophore iron reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,605,629	0	A	G	98.7%	268.0 / ‑4.5	78	V12A (GTC→GCC)	fhuF	hydroxamate siderophore iron reductase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (34/43); total (35/43)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTATCTGGCTTGCAAATGATAAAAATTA  >  NC_000913/4605492‑4605760

ccAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCa                                                                                                                                   >  2:159115/1‑140 (MQ=255)

 cAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCAt                                                                                                                                  <  1:55293/140‑1 (MQ=255)

 cAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCAt                                                                                                                                  <  1:812663/140‑1 (MQ=255)

 cAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCAt                                                                                                                                  <  1:558992/140‑1 (MQ=255)

  aGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATc                                                                                                                                 >  1:856214/1‑140 (MQ=255)

      tCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCa                                                                                                                             >  2:779802/1‑140 (MQ=255)

        aTGGCGTTAAGTGGGGCAGGTTAATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGACATCTTCATa                                                                                                                           >  2:60733/1‑140 (MQ=255)

           gCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATagag                                                                                                                        <  1:702893/140‑1 (MQ=255)

            cGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCCCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGc                                                                                                                       <  2:798349/140‑1 (MQ=255)

                aaGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTg                                                                                                                   >  2:275957/1‑140 (MQ=255)

                 aGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGc                                                                                                                  >  2:859936/1‑140 (MQ=255)

                   tGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCgg                                                                                                                <  2:835606/140‑1 (MQ=255)

                     gggCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGaa                                                                                                              <  1:598675/140‑1 (MQ=255)

                      ggCAGGTTCATCCAGGCGGATAAACCCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGCCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAAc                                                                                                             >  2:546474/1‑140 (MQ=255)

                           gTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAg                                                                                                        >  1:15463/1‑140 (MQ=255)

                            ttCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAgg                                                                                                       <  1:749108/140‑1 (MQ=255)

                            ttCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAgg                                                                                                       <  1:159115/140‑1 (MQ=255)

                                   aGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAAt                                                                                                <  1:405579/140‑1 (MQ=255)

                                   aGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAAt                                                                                                <  2:15463/140‑1 (MQ=255)

                                     gCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCg                                                                                              >  1:692663/1‑140 (MQ=255)

                                     gCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCg                                                                                              <  1:1032705/140‑1 (MQ=255)

                                      cGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCgg                                                                                             <  1:859936/140‑1 (MQ=255)

                                         aTAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGAt                                                                                          >  2:708557/1‑140 (MQ=255)

                                         aTAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGAt                                                                                          >  1:788882/1‑140 (MQ=255)

                                          tAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATa                                                                                         >  1:673960/1‑140 (MQ=255)

                                            aaCTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGt                                                                                       <  1:779802/140‑1 (MQ=255)

                                             aCTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTa                                                                                      <  1:708557/140‑1 (MQ=255)

                                               tCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAAt                                                                                    >  1:1052654/1‑140 (MQ=255)

                                                ccAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATa                                                                                         >  2:534488/1‑134 (MQ=255)

                                                ccAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATa                                                                                         <  1:534488/134‑1 (MQ=255)

                                                 cAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCt                                                                                  >  1:48160/1‑140 (MQ=255)

                                                 cAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCt                                                                                  <  2:788882/140‑1 (MQ=255)

                                                   gCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTaa                                                                                >  2:1083584/1‑140 (MQ=255)

                                                   gCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTaa                                                                                >  2:615463/1‑140 (MQ=255)

                                                   gCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTaa                                                                                >  2:788910/1‑140 (MQ=255)

                                                        tGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgat                                                                           <  2:277321/140‑1 (MQ=255)

                                                         gTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTaa                                                                                >  2:951288/1‑134 (MQ=255)

                                                         gTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTaa                                                                                <  1:951288/134‑1 (MQ=255)

                                                         gTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgata                                                                          <  2:71218/140‑1 (MQ=255)

                                                         gTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgata                                                                          <  2:692663/140‑1 (MQ=255)

                                                         gTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgata                                                                          >  2:166793/1‑140 (MQ=255)

                                                           tCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgataat                                                                        >  2:912976/1‑140 (MQ=255)

                                                           tCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgataat                                                                        <  2:58596/140‑1 (MQ=255)

                                                           tCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgataat                                                                        <  2:194416/140‑1 (MQ=255)

                                                             aCGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgataatga                                                                      <  2:351391/140‑1 (MQ=255)

                                                              cGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgataat                                                                        >  1:542624/1‑137 (MQ=255)

                                                              cGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgataat                                                                        <  2:542624/137‑1 (MQ=255)

                                                                  gTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCt                                                                 >  2:17288/1‑140 (MQ=255)

                                                                  gTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCt                                                                 >  2:184972/1‑140 (MQ=255)

                                                                    tttGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTaa                                                               >  2:345432/1‑140 (MQ=255)

                                                                       gCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCa                                                            <  1:864268/140‑1 (MQ=255)

                                                                        cGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCAt                                                           <  1:1139458/140‑1 (MQ=255)

                                                                         gATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATa                                                          >  1:237508/1‑140 (MQ=255)

                                                                           tcgtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGc                                                        >  2:948621/1‑140 (MQ=255)

                                                                             gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGa                                                      <  1:184972/140‑1 (MQ=255)

                                                                             gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGa                                                      >  1:114191/1‑140 (MQ=255)

                                                                             gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGa                                                      <  1:948621/140‑1 (MQ=255)

                                                                             gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGa                                                      <  1:1083584/140‑1 (MQ=255)

                                                                             gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGa                                                      <  2:237508/140‑1 (MQ=255)

                                                                             gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGa                                                      <  1:788910/140‑1 (MQ=255)

                                                                             gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGa                                                      <  1:916952/140‑1 (MQ=255)

                                                                              tcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGAt                                                     <  2:796444/140‑1 (MQ=255)

                                                                                  ccGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGt                                                 <  2:856214/140‑1 (MQ=255)

                                                                                   cGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGtt                                                <  1:345432/140‑1 (MQ=255)

                                                                                          cTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGa                                         >  2:1014275/1‑140 (MQ=255)

                                                                                              cgcAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGc                                     >  1:53483/1‑140 (MQ=255)

                                                                                              cgcAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGc                                     >  2:360657/1‑140 (MQ=255)

                                                                                               gcAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCa                                    <  1:166793/140‑1 (MQ=255)

                                                                                                cAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCaa                                   <  2:53483/140‑1 (MQ=255)

                                                                                                  aGCGTGGGATCCTGCGGCTGGAGATGCGTTAGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCa                                    <  1:518685/137‑1 (MQ=255)

                                                                                                  aGCGTGGGATCCTGCGGCTGGAGATGCGTTAGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCa                                    >  2:518685/1‑137 (MQ=255)

                                                                                                             cTGCGGCTGGAGATGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTATCTgg                      >  1:651711/1‑140 (MQ=255)

                                                                                                                      gagaTGCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTATCTGGCTTGCAAAt             >  2:1007412/1‑140 (MQ=255)

                                                                                                                           gCGTTCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTATCTGGCTTGCAAATGATaa        <  1:1073051/140‑1 (MQ=255)

                                                                                                                              ttCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTATCTGGCTTGCAAATGATaaaaa     <  1:1014275/140‑1 (MQ=255)

                                                                                                                               tCGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTATCTGGCTTGCAAATGATAAAAAt    <  1:546474/140‑1 (MQ=255)

                                                                                                                                cGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTATCTGGCTTGCAAATGATAAAAAtt   >  1:389179/1‑140 (MQ=255)

                                                                                                                                cGCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTATCTGGCTTGCAAATGATAAAAAtt   >  2:1146549/1‑140 (MQ=255)

                                                                                                                                 gCCAGATGGCATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTATCTGGCTTGCAAATGATAAAAATTa  <  2:651711/140‑1 (MQ=255)

CCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTATCTGGCTTGCAAATGATAAAAATTA  >  NC_000913/4605492‑4605760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A