Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,436,053	C→T	L283F (CTC→TTC)	rsmB →	16S rRNA m(5)C967 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,436,053	0	C	T	100.0%	189.4 / NA	54	L283F (CTC→TTC)	rsmB	16S rRNA m(5)C967 methyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/25); total (29/25)

TGCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTT  >  NC_000913/3435918‑3436182

tGCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCttctc                                                                                                                               <  1:130017/140‑1 (MQ=255)

  cATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTtctctc                                                                                                                             <  2:243506/140‑1 (MQ=255)

                cgcCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTtctctc                                                                                                                             >  2:60047/1‑126 (MQ=255)

                cgcCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTtctctc                                                                                                                             <  1:60047/126‑1 (MQ=255)

                cgcCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAAt                                                                                                               >  2:357027/1‑140 (MQ=255)

                 gcCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAAtt                                                                                                              >  1:159857/1‑140 (MQ=255)

                 gcCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAAtt                                                                                                              >  2:310100/1‑140 (MQ=255)

                      aaaaCGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTaaaa                                                                                                         <  1:357027/140‑1 (MQ=255)

                               aaCACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGt                                                                                                >  1:556422/1‑140 (MQ=255)

                                     ttttGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAg                                                                                          >  2:640917/1‑140 (MQ=255)

                                       ttGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTTGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGc                                                                                        <  1:745986/140‑1 (MQ=255)

                                         ggATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGTTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGa                                                                                      <  2:763733/140‑1 (MQ=255)

                                         ggATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGa                                                                                      <  2:507200/140‑1 (MQ=255)

                                           aTCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGAcc                                                                                    <  1:485934/140‑1 (MQ=255)

                                            tCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCg                                                                                   <  2:556422/140‑1 (MQ=255)

                                                tgtgCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGaa                                                                               >  1:154514/1‑140 (MQ=255)

                                                    ccgccCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGAATGAAGGCGACCGTGAAACaa                                                                           >  2:78469/1‑140 (MQ=255)

                                                      gccCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGGGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAgg                                                                         >  2:715919/1‑140 (MQ=255)

                                                       cccccGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACaa                                                                           <  1:18058/137‑1 (MQ=255)

                                                       cccccGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACaa                                                                           >  2:18058/1‑137 (MQ=255)

                                                       cccccGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGt                                                                        >  2:311481/1‑140 (MQ=255)

                                                       cccccGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGt                                                                        >  2:227713/1‑140 (MQ=255)

                                                        ccccGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTg                                                                       >  2:527259/1‑140 (MQ=255)

                                                         cccGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGa                                                                      <  1:640917/140‑1 (MQ=255)

                                                                 tAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGtt                                                              <  1:310100/140‑1 (MQ=255)

                                                                  aaacCAACGCGTAGCCTTGAGGTGGCTCCTGAAGCGCAGGTTGTTGCGGTTGATATCTACGAACAGCGGTTCTCTCGGGTTTACGTCAGTTTAATGCGCCTTGGTATGAAGTCGACCGCTAAGCAAGGTGATGGCCgatc                                                             <  2:282958/140‑4 (MQ=255)

                                                                          gCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATg                                                                    >  1:2968/1‑125 (MQ=255)

                                                                          gCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATg                                                                    <  2:2968/125‑1 (MQ=255)

                                                                           cATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCa                                                    <  1:919234/140‑1 (MQ=255)

                                                                                   tGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGtgtg                                            <  1:715919/140‑1 (MQ=255)

                                                                                    gAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTgg                                           >  1:721465/1‑140 (MQ=255)

                                                                                      ggTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCg                                         >  2:883112/1‑140 (MQ=255)

                                                                                        tGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAg                                       <  1:224742/140‑1 (MQ=255)

                                                                                          gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAGTGGTGTGGCGAGCa                                     >  2:591724/1‑140 (MQ=255)

                                                                                          gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGc                                      <  2:445399/139‑1 (MQ=255)

                                                                                          gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGc                                      >  1:445399/1‑139 (MQ=255)

                                                                                          gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCa                                     >  2:251458/1‑140 (MQ=255)

                                                                                          gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCa                                     >  1:266053/1‑140 (MQ=255)

                                                                                          gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCa                                     >  2:333601/1‑140 (MQ=255)

                                                                                          gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCa                                     >  2:270204/1‑140 (MQ=255)

                                                                                          gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCa                                     >  1:416363/1‑140 (MQ=255)

                                                                                            aCCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAAc                                   <  1:568205/140‑1 (MQ=255)

                                                                                               aGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGt                                <  2:721465/140‑1 (MQ=255)

                                                                                                   gcgcAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGa                            <  1:344783/140‑1 (MQ=255)

                                                                                                      cAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCg                         <  1:883112/140‑1 (MQ=255)

                                                                                                      cAGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCg                         >  1:374729/1‑140 (MQ=255)

                                                                                                       aGGTTGTTGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGc                        <  2:374729/140‑1 (MQ=255)

                                                                                                         gttgttGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCAt                      <  1:270204/140‑1 (MQ=255)

                                                                                                         gttgttGCGGTTGATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCAt                      <  1:333601/140‑1 (MQ=255)

                                                                                                                     gATATCGACGAACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATgc          >  2:884871/1‑140 (MQ=255)

                                                                                                                            acgaACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGt   >  2:246393/1‑140 (MQ=255)

                                                                                                                            acgaACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGt   >  1:938479/1‑140 (MQ=255)

                                                                                                                             cgaACAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGtt  >  1:392893/1‑140 (MQ=255)

                                                                                                                                aCAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATgcg         >  2:497905/1‑130 (MQ=255)

                                                                                                                                aCAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATgcg         <  1:497905/130‑1 (MQ=255)

                                                                                                                                 cAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGcc       <  1:846829/131‑1 (MQ=255)

                                                                                                                                 cAGCGCTTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGcc       >  2:846829/1‑131 (MQ=255)

TGCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTT  >  NC_000913/3435918‑3436182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A