Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,637,751	A→C	N37T (AAC→ACC)	pitA →	metal phosphate:H(+) symporter PitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,637,751	0	A	C	100.0%	206.8 / NA	57	N37T (AAC→ACC)	pitA	metal phosphate:H(+) symporter PitA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (32/25); total (32/25)

CATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGA  >  NC_000913/3637615‑3637885

cATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGc                                                                                                                                     >  2:17950/1‑140 (MQ=255)

      cTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGc                                                                                                                               <  2:74957/140‑1 (MQ=255)

        cAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCaa                                                                                                                             <  1:112559/140‑1 (MQ=255)

           aaTGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCg                                                                                                                          <  1:17950/140‑1 (MQ=255)

           aaTGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCg                                                                                                                          <  1:53000/140‑1 (MQ=255)

                cGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATc                                                                                                                     >  2:881528/1‑140 (MQ=255)

                cGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATc                                                                                                                     >  2:808599/1‑140 (MQ=255)

                 gTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCt                                                                                                                    >  2:285797/1‑140 (MQ=255)

                     cGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATAc                                                                                                                >  2:127625/1‑140 (MQ=255)

                         cTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCcgc                                                                                                            <  1:127625/140‑1 (MQ=255)

                           aTGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCcgcgc                                                                                                          <  1:412939/140‑1 (MQ=255)

                                    ttgtttgCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTtc                                                                                                 >  1:708565/1‑140 (MQ=255)

                                     tgtttgCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTtct                                                                                                <  1:931083/140‑1 (MQ=255)

                                      gtttgCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTtctc                                                                                               >  2:688385/1‑140 (MQ=255)

                                       tttgCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCa                                                                                              >  2:170706/1‑140 (MQ=255)

                                        ttgCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAg                                                                                             <  1:881528/140‑1 (MQ=255)

                                           cTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTc                                                                                          >  2:811431/1‑140 (MQ=255)

                                                   gATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGt                                                                                  >  1:283746/1‑140 (MQ=255)

                                                   gATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGt                                                                                  >  2:230408/1‑140 (MQ=255)

                                                             ccGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGgcggcg                                                                        <  1:774495/140‑1 (MQ=255)

                                                                  cTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTAtt                                                                   >  1:887914/1‑140 (MQ=255)

                                                                             gcttgcACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTgg                                                        <  2:293990/140‑1 (MQ=255)

                                                                               ttgcACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGgt                                                      >  1:686847/1‑140 (MQ=255)

                                                                               ttgcACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGgt                                                      >  1:49654/1‑140 (MQ=255)

                                                                               ttgcACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGgt                                                      >  1:620016/1‑140 (MQ=255)

                                                                               ttgcACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCAGTGGTTATGGCGGCGGTATTCAACTTTTTgggg                                                      >  1:820177/1‑139 (MQ=255)

                                                                                tgcACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGgtg                                                     >  1:351544/1‑140 (MQ=255)

                                                                                tgcACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGgtg                                                     >  1:555641/1‑140 (MQ=255)

                                                                                      ggCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGc                                               <  1:515380/140‑1 (MQ=255)

                                                                                      ggCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGc                                               <  1:797185/140‑1 (MQ=255)

                                                                                       gCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCt                                              >  2:881269/1‑140 (MQ=255)

                                                                                          tttGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTggg                                           <  1:170706/140‑1 (MQ=255)

                                                                                               gCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTc                                      <  2:686847/140‑1 (MQ=255)

                                                                                               gCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTc                                      <  2:620016/140‑1 (MQ=255)

                                                                                                 tGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTg                                    >  2:523936/1‑140 (MQ=255)

                                                                                                   ttCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCTACTTTTTGGGTGTTTTTCTGGGGGGTCTGAg                                  >  2:210848/1‑140 (MQ=255)

                                                                                                       aCGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGtt                              <  2:283746/140‑1 (MQ=255)

                                                                                                            gCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTa                         <  2:49654/140‑1 (MQ=255)

                                                                                                             ccATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGc                            <  1:746721/136‑1 (MQ=255)

                                                                                                             ccATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGc                            >  2:746721/1‑136 (MQ=255)

                                                                                                             ccATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTa                         >  1:960396/1‑139 (MQ=255)

                                                                                                             ccATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTa                         <  2:960396/139‑1 (MQ=255)

                                                                                                             ccATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTAt                        >  1:734485/1‑140 (MQ=255)

                                                                                                             ccATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTAt                        >  1:406879/1‑140 (MQ=255)

                                                                                                              cATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATg                       <  2:708565/140‑1 (MQ=255)

                                                                                                               aTCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTAAGTGTTGCCTa                         >  2:833313/1‑137 (MQ=255)

                                                                                                               aTCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCACGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTa                         <  1:833313/137‑1 (MQ=255)

                                                                                                                tCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGcc                     >  1:716419/1‑140 (MQ=255)

                                                                                                                 cAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCa                    <  2:406879/140‑1 (MQ=255)

                                                                                                                  aaTGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCAt                   <  2:887914/140‑1 (MQ=255)

                                                                                                                       tttCCATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGGGGTCTGAGTGTTGCCTATGCCATTGTGc              >  1:509876/1‑140 (MQ=255)

                                                                                                                          ccATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCata           >  2:95637/1‑140 (MQ=255)

                                                                                                                           cATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATg                       >  2:90644/1‑127 (MQ=255)

                                                                                                                           cATGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATg                       <  1:90644/127‑1 (MQ=255)

                                                                                                                            aTGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATAtg         <  2:555641/140‑1 (MQ=255)

                                                                                                                            aTGACACAGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATAtg         <  2:351544/140‑1 (MQ=255)

                                                                                                                                cacaGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATAtgctgc     >  2:541450/1‑140 (MQ=255)

                                                                                                                                   aGCCACCGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGa  >  2:837389/1‑140 (MQ=255)

CATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGA  >  NC_000913/3637615‑3637885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A