Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 182 57.1 1926220 99.1% 1908884 138.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,051,495 A→G N50S (AAC→AGC)  yihI → Der GTPase‑activating protein YihI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,051,4950AG100.0% 155.8 / NA 45N50S (AAC→AGC) yihIDer GTPase‑activating protein YihI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/23);  total (22/23)

AACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC  >  NC_000913/4051365‑4051627
                                                                                                                                  |                                                                                                                                    
aaCTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTc                                                                                                                             >  2:90051/1‑140 (MQ=255)
 aCTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCa                                                                                                                            <  2:793574/140‑1 (MQ=255)
     aCGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGgca                                                                                                                        >  1:522299/1‑140 (MQ=255)
       gcagcaAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGgcagc                                                                                                                      >  2:581866/1‑140 (MQ=255)
        cagcaAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGgcagca                                                                                                                     <  1:90051/140‑1 (MQ=255)
             aaGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGt                                                                                                                              >  1:257314/1‑126 (MQ=255)
             aaGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGt                                                                                                                              <  2:257314/126‑1 (MQ=255)
                  cATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGaa                                                                                                           <  2:10474/140‑1 (MQ=255)
                  cATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGaa                                                                                                           <  2:356898/140‑1 (MQ=255)
                    tGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACg                                                                                                         >  1:590724/1‑140 (MQ=255)
                     gCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAgg                                                                                                                          <  2:491185/122‑1 (MQ=255)
                     gCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAgg                                                                                                                          >  1:491185/1‑122 (MQ=255)
                          aGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCaa                                                                                                   >  1:636347/1‑140 (MQ=255)
                          aGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCaa                                                                                                   >  2:894007/1‑140 (MQ=255)
                          aGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCaa                                                                                                   >  2:26894/1‑140 (MQ=255)
                          aGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCaa                                                                                                   >  2:258223/1‑140 (MQ=255)
                           gcgcGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCaaa                                                                                                  <  1:894007/140‑1 (MQ=255)
                                tCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATc                                                                                             <  1:699243/140‑1 (MQ=255)
                                tCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATc                                                                                             <  1:811903/140‑1 (MQ=255)
                                  gAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCa                                                                                           <  2:805478/140‑1 (MQ=255)
                                                 agCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTaaa                                                                            <  1:851686/140‑1 (MQ=255)
                                                     ggATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAAccc                                                                        <  1:258223/140‑1 (MQ=255)
                                                        tCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTa                                                                     <  2:522299/140‑1 (MQ=255)
                                                         cAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTAt                                                                    <  2:636347/140‑1 (MQ=255)
                                                           ggAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTc                                                                  >  1:400166/1‑140 (MQ=255)
                                                           ggAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTc                                                                  >  2:416346/1‑140 (MQ=255)
                                                            gAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAAc                                                                          >  1:15470/1‑131 (MQ=255)
                                                            gAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAAc                                                                          <  2:15470/131‑1 (MQ=255)
                                                             aaGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTAt                                                                    <  1:688969/136‑1 (MQ=255)
                                                             aaGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTAt                                                                    >  2:688969/1‑136 (MQ=255)
                                                             aaGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCa                                                                >  1:121037/1‑140 (MQ=255)
                                                                cTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTg                                                             >  1:315454/1‑140 (MQ=255)
                                                                      aTCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTg                                                       >  2:629745/1‑140 (MQ=255)
                                                                          cAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTg                                                   <  1:629745/140‑1 (MQ=255)
                                                                           aaGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGa                                                  >  1:728249/1‑140 (MQ=255)
                                                                                   gAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCa                                          <  1:652405/140‑1 (MQ=255)
                                                                                        aaCGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCaaa                                     <  1:733525/140‑1 (MQ=255)
                                                                                        aaCGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCaaa                                     <  2:121037/140‑1 (MQ=255)
                                                                                              gtGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGcac                               <  1:416346/140‑1 (MQ=255)
                                                                                                      gcgcCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGaa                       <  1:581866/140‑1 (MQ=255)
                                                                                                      gcgcCGGGCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGaa                       >  2:950073/1‑140 (MQ=255)
                                                                                                            ggCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTga                 >  1:418376/1‑140 (MQ=255)
                                                                                                             gCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCTAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTgag                <  2:100298/140‑1 (MQ=255)
                                                                                                                 ccGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAc            >  1:609097/1‑140 (MQ=255)
                                                                                                                         cgggcggAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCtt    <  2:400166/140‑1 (MQ=255)
                                                                                                                           ggcggAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTc  >  1:470569/1‑140 (MQ=255)
                                                                                                                                  |                                                                                                                                    
AACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTCATGCGCCGGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC  >  NC_000913/4051365‑4051627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A