Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,456,162	A→G	E126E (GAA→GAG)	mpl →	UDP‑N‑acetylmuramate‑‑L‑alanyl‑gamma‑D‑glutamyl‑ meso‑2,6‑diaminoheptanedioate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,456,162	0	A	G	100.0%	207.0 / NA	58	E126E (GAA→GAG)	mpl	UDP‑N‑acetylmuramate‑‑L‑alanyl‑gamma‑D‑glutamyl‑ meso‑2,6‑diaminoheptanedioate ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (34/24); total (34/24)

ACTGGAAAAAAATATCCCTTATATGTCAGGTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAACAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTGCGCCTTCTTCGACAAACGC  >  NC_000913/4456027‑4456294

aCTGGAAAAAAATATCCCTTATATGTCAGGTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAgt                                                                                                                                  <  1:521209/140‑1 (MQ=255)

   ggAAAAAAATATCCCTTATATGTCAGGTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGtgtg                                                                                                                               <  1:541657/140‑1 (MQ=255)

    gAAAAAAATATCCCTTATATGTCAGGTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTgg                                                                                                                              >  1:274703/1‑140 (MQ=255)

         aaacatCCCTTATATGTCAGGTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACa                                                                                                                         >  2:919130/5‑140 (MQ=255)

              tCCCTTATATGTCAGGTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCg                                                                                                                    >  2:612166/1‑140 (MQ=255)

                 cTTATATGTCAGGTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGc                                                                                                                 <  2:274703/140‑1 (MQ=255)

                  ttATATGTCAGGTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCt                                                                                                                <  1:843552/140‑1 (MQ=255)

                   tataTGTCAGGTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCtt                                                                                                               >  2:760798/1‑140 (MQ=255)

                            ggTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATcgg                                                                                                      >  2:929867/1‑140 (MQ=255)

                            ggTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATcgg                                                                                                      >  2:498012/1‑140 (MQ=255)

                            ggTCCACAGTAGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATcgg                                                                                                      >  2:490628/1‑140 (MQ=255)

                             gTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATcggc                                                                                                     >  1:917647/1‑140 (MQ=255)

                             gTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATcggc                                                                                                     >  2:339040/1‑140 (MQ=255)

                              tCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATcggcg                                                                                                    <  2:917647/140‑1 (MQ=255)

                                       gCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCgg                                                                                           >  2:583876/1‑140 (MQ=255)

                                       gCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCgg                                                                                           >  2:744709/1‑140 (MQ=255)

                                        cTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGTATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCggg                                                                                          >  1:898088/1‑140 (MQ=255)

                                         tGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCgggg                                                                                         >  2:355277/1‑140 (MQ=255)

                                          gCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGTATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGa                                                                                        <  2:898088/140‑1 (MQ=255)

                                          gCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGa                                                                                        >  1:318120/1‑140 (MQ=255)

                                          gCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAACGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGa                                                                                        <  2:846268/140‑1 (MQ=255)

                                           cACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGaa                                                                                       >  1:769865/1‑140 (MQ=255)

                                              gATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTATAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACtt                                                                                    <  1:243228/140‑1 (MQ=255)

                                                    gtgCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGt                                                                              <  2:116695/140‑1 (MQ=255)

                                                    gtgCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGt                                                                              <  1:339040/140‑1 (MQ=255)

                                                     tgctgcGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGtt                                                                             >  2:608129/1‑140 (MQ=255)

                                                     tgctgcGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGtt                                                                             >  1:758208/1‑140 (MQ=255)

                                                         gcgcGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCgg                                                                         <  1:919130/140‑1 (MQ=255)

                                                         gcgcGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCgg                                                                         <  1:583876/140‑1 (MQ=255)

                                                          cgcgACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGc                                                                        >  1:546302/1‑140 (MQ=255)

                                                          cgcgACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGc                                                                        >  2:283511/1‑140 (MQ=255)

                                                            cgACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTc                                                                      >  2:58275/1‑140 (MQ=255)

                                                                     ggTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCg                                                             <  1:760798/140‑1 (MQ=255)

                                                                      gTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGa                                                            >  1:908683/1‑140 (MQ=255)

                                                                          tGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGc                                                        <  2:6810/140‑1 (MQ=255)

                                                                           ggCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCg                                                       >  2:900054/1‑140 (MQ=255)

                                                                                 tGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGActtct                                                 >  1:950612/1‑140 (MQ=255)

                                                                                           cacGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGa                                       <  1:608129/140‑1 (MQ=255)

                                                                                            acGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGaa                                      <  1:58275/140‑1 (MQ=255)

                                                                                            acGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGaa                                      <  2:546302/140‑1 (MQ=255)

                                                                                              ggCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGc                                    >  1:89971/1‑140 (MQ=255)

                                                                                                cAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCgg                                  <  2:769865/140‑1 (MQ=255)

                                                                                                cAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCgg                                  <  1:744709/140‑1 (MQ=255)

                                                                                                 aaaaCCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGa                                 >  2:100638/1‑140 (MQ=255)

                                                                                                 aaaaCCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGa                                 >  1:571098/1‑140 (MQ=255)

                                                                                                  aaaCCACCACCGCGGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGAt                                <  2:571098/140‑1 (MQ=255)

                                                                                                              cgGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTgc                    >  1:845888/1‑140 (MQ=255)

                                                                                                              cgGGAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTgc                    >  1:893442/1‑140 (MQ=255)

                                                                                                                ggAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTgcg                   >  1:962167/1‑139 (MQ=255)

                                                                                                                ggAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTgcg                   <  2:962167/139‑1 (MQ=255)

                                                                                                                 gAATGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTGCGcc                 <  1:498012/140‑1 (MQ=255)

                                                                                                                   aTGGCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTGCGCctt               >  2:223470/1‑140 (MQ=255)

                                                                                                                     ggCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTGCGCcttct             >  2:548356/1‑140 (MQ=255)

                                                                                                                      gCGACCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTGCGCcttctt            >  2:312111/1‑140 (MQ=255)

                                                                                                                         aCCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTgcg                   <  1:42024/130‑1 (MQ=255)

                                                                                                                         aCCTGGATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCAGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTgcg                   >  2:42024/1‑130 (MQ=255)

                                                                                                                             ggATTCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTGCGCCTTCTTCGACaaa     <  2:950612/140‑1 (MQ=255)

                                                                                                                                ttCTGGAGCAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTGCGCCTTCTTCGACAAACGc  >  1:275091/1‑140 (MQ=255)

ACTGGAAAAAAATATCCCTTATATGTCAGGTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGCCGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAACAGTGTGGTTACAAACCGGGCTTTGTAATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCTCATCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGATGAGTATGACTGCGCCTTCTTCGACAAACGC  >  NC_000913/4456027‑4456294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A