Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,511,128	A→G	R110R (CGT→CGC)	fecE ←	ferric citrate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,511,128	0	A	G	100.0%	204.3 / NA	58	R110R (CGT→CGC)	fecE	ferric citrate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/34); total (24/34)

GGCCAGGACCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGG  >  NC_000913/4510996‑4511264

ggCCAGGACCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGccccc                                                                                                                                   >  2:328051/1‑140 (MQ=255)

 gCCAGGACCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCa                                                                                                                                  <  2:852353/140‑1 (MQ=255)

      gACCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCG‑CCCCAGAGTGa                                                                                                                            >  1:752424/1‑140 (MQ=255)

       aCCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGa                                                                                                                            <  1:729191/140‑1 (MQ=255)

       aCCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGa                                                                                                                            <  2:57411/140‑1 (MQ=255)

       aCCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGa                                                                                                                            <  2:530186/140‑1 (MQ=255)

           ttGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGcc                                                                                                                                      <  2:8285/126‑1 (MQ=255)

           ttGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGcc                                                                                                                                      >  1:8285/1‑126 (MQ=255)

           ttGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGc                                                                                                                        >  2:555827/1‑140 (MQ=255)

           ttGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGc                                                                                                                        >  1:42230/1‑140 (MQ=255)

           ttGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGc                                                                                                                        >  2:183671/1‑140 (MQ=255)

             gCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCa                                                                                                                      <  1:191317/140‑1 (MQ=255)

             gCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCa                                                                                                                      <  1:573902/140‑1 (MQ=255)

             gCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCa                                                                                                                      <  2:481471/140‑1 (MQ=255)

             gCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCa                                                                                                                      <  1:672567/140‑1 (MQ=255)

                aGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCG‑‑GAACGGCAAGATGATTGAACCGGGACTGGTTCATGGCGACTTTAACTCGAGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAACGAGGGAt                                                                                                                 >  2:351638/1‑140 (MQ=255)

                       cgcgcTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGa                                                                                                            >  2:392459/1‑140 (MQ=255)

                        gcgctggcgctgACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATAGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGAc                                                                                                           >  1:364320/1‑140 (MQ=255)

                            tggcgctgACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATa                                                                                                       <  2:957521/140‑1 (MQ=255)

                              gcgctgACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACg                                                                                                     <  1:555827/140‑1 (MQ=255)

                                   gACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAAcc                                                                                                <  1:328051/140‑1 (MQ=255)

                                     ccgccgGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAg                                                                                              <  1:752294/140‑1 (MQ=255)

                                      cgccgGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAg                                                                                              >  2:491417/1‑139 (MQ=255)

                                      cgccgGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAg                                                                                              <  1:491417/139‑1 (MQ=255)

                                        ccgGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTc                                                                                           <  1:392459/140‑1 (MQ=255)

                                        ccgGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTc                                                                                           >  1:53514/1‑140 (MQ=255)

                                                cTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAg                                                                                              <  1:393098/129‑1 (MQ=255)

                                                cTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAg                                                                                              >  2:393098/1‑129 (MQ=255)

                                                        aCCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCG‑CCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCct                                                                          <  2:752424/140‑1 (MQ=255)

                                                         ccGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCct                                                                          <  2:749357/140‑1 (MQ=255)

                                                          cgacgaACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCctc                                                                         <  2:277268/140‑1 (MQ=255)

                                                          cgacgaACGGCAAGATGATTGATCCGGGTCTGGTTCATAGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCctc                                                                         <  2:364320/140‑1 (MQ=255)

                                                            cggaACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTg                                                                       <  1:351638/138‑1 (MQ=255)

                                                            acgaACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTg                                                                       <  2:53514/140‑1 (MQ=255)

                                                             cgaACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACt                                                                                    >  2:129124/1‑126 (MQ=255)

                                                             cgaACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACt                                                                                    <  1:129124/126‑1 (MQ=255)

                                                                            ttgatCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTg                                                       >  2:445974/1‑140 (MQ=255)

                                                                                  cGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcag                                                 >  2:129682/1‑140 (MQ=255)

                                                                                  cGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcag                                                 >  1:772367/1‑140 (MQ=255)

                                                                                    ggTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgc                                                   <  1:618838/136‑1 (MQ=255)

                                                                                    ggTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgc                                                   >  2:618838/1‑136 (MQ=255)

                                                                                          gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGTCCATACGAGACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGaa                                          >  2:637784/1‑139 (MQ=255)

                                                                                          gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGaaa                                         >  1:206372/1‑140 (MQ=255)

                                                                                          gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGaa                                          <  1:637784/139‑1 (MQ=255)

                                                                                               tGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTg                                    >  2:96030/1‑140 (MQ=255)

                                                                                               tGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTg                                    <  1:920196/140‑1 (MQ=255)

                                                                                                ggCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcag                                                 <  1:102305/126‑1 (MQ=255)

                                                                                                ggCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcag                                                 >  2:102305/1‑126 (MQ=255)

                                                                                                 gCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCg                                  <  1:183671/140‑1 (MQ=255)

                                                                                                  cGACATTAACTCGTGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCgg                                 <  2:620761/140‑1 (MQ=255)

                                                                                                               tGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGcgcg                    >  2:182699/1‑140 (MQ=255)

                                                                                                               tGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGcgcg                    >  2:524419/1‑140 (MQ=255)

                                                                                                               tGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGcgcg                    >  2:673689/1‑140 (MQ=255)

                                                                                                               tGCATTGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGcgcg                    <  2:772367/140‑1 (MQ=255)

                                                                                                                    tGTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATgag               <  1:182699/140‑1 (MQ=255)

                                                                                                                     gTCTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATgaga              <  2:881257/140‑1 (MQ=255)

                                                                                                                      tCTTCAGCGGAGAGGCGCCCCCATAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGagag             >  2:28306/1‑140 (MQ=255)

                                                                                                                       cTTCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc            <  1:129682/140‑1 (MQ=255)

                                                                                                                         tCAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCat          <  2:883048/140‑1 (MQ=255)

                                                                                                                          cAGCGGAGAGGCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCata         >  2:67253/1‑140 (MQ=255)

                                                                                                                               gagaggCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTAt    <  1:524419/140‑1 (MQ=255)

                                                                                                                                agaggCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCa           >  1:411263/1‑132 (MQ=255)

                                                                                                                                agaggCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCa           <  2:411263/132‑1 (MQ=255)

                                                                                                                                agaggCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATg   >  2:229463/1‑140 (MQ=255)

                                                                                                                                agaggCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGAT‑CCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATgg  >  2:635066/1‑140 (MQ=255)

                                                                                                                                agagaCGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATg   >  2:558626/1‑140 (MQ=255)

GGCCAGGACCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGG  >  NC_000913/4510996‑4511264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A