Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F28 I1 R1
|
182 |
57.1 |
1926220 |
99.1% |
1908884 |
138.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,606,215 |
(G)8→9 |
noncoding (72/87 nt) |
leuP ← |
tRNA‑Leu |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,606,207 | 1 | . | G | 86.5%
| 107.6
/ 11.0
| 37 | noncoding (80/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (2/3); new base G (16/16); total (18/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 |
ATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACT > NC_000913/4606074‑4606333
|
aTAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑gggggg > 1:272990/1‑140 (MQ=255)
aaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑gggggggg > 1:907952/1‑140 (MQ=255)
aTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑ggggggggg > 2:910793/1‑139 (MQ=255)
gTGCGAGGGGGGGGACTTGAACCCCCACTTTCGTAAGGGCACTAACCCCTGAAGCTAGCGCGGCTACCAATTCCGCCGCCTTCGCACAGTCATCTTACTTTTTTTGATAACGCCTCGTTTGGTGCg‑g‑gggggggggactt < 2:907952/140‑6 (MQ=21)
gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑gggggggggactt < 1:582641/140‑6 (MQ=255)
gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGa < 2:816926/140‑1 (MQ=255)
gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGa > 2:761826/1‑140 (MQ=255)
gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGa < 1:647547/140‑1 (MQ=255)
cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGaa < 1:31207/140‑1 (MQ=255)
gggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAAcccc < 1:761826/140‑1 (MQ=255)
gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCa > 2:320409/1‑140 (MQ=255)
ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCAc < 1:320409/140‑1 (MQ=255)
ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGt > 1:882991/1‑140 (MQ=255)
gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGCG‑GGGGGGGG‑A‑CTTGAACCCCCACGTCCGTaa > 2:652108/1‑140 (MQ=255)
cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTc < 2:519465/130‑1 (MQ=255)
cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTc > 1:519465/1‑130 (MQ=255)
cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACt < 1:544228/140‑1 (MQ=255)
aCGTCCGTAAGGACACTAACACCTGAAGCTATCTCGCCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGGGGG‑AGCTTTAACCCCCACGTCCGTAAGAAGACTa > 2:155499/1‑140 (MQ=37)
cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCTCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTaa < 1:155499/140‑1 (MQ=255)
acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg < 1:241689/140‑1 (MQ=255)
tAACCCCTGAACCTAGTGTGTCTCCCAATTCGGCAGCCTTCGCACACTTATCTTACTTTTTTTGATAACGACTCGTTTAGTGCG‑ACGGGGGGGG‑A‑CTTGAACACCCACCTCCGTAAGAACACTAACACCTGAAGCTAgcg < 2:146366/140‑1 (MQ=18)
gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCTTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCTCGTCTAcc > 1:146366/1‑140 (MQ=255)
aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc > 1:725218/1‑140 (MQ=255)
aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc > 2:528193/1‑140 (MQ=255)
aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CGTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc > 1:768702/1‑140 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 1:652108/140‑1 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 2:882991/140‑1 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGGGGGTA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 1:172556/140‑1 (MQ=255)
gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc > 2:182997/1‑140 (MQ=255)
cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg > 2:583421/1‑140 (MQ=255)
cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CGTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg < 2:768702/140‑1 (MQ=255)
cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc > 2:134371/1‑140 (MQ=255)
ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg > 2:814457/1‑140 (MQ=255)
ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc > 2:703850/1‑133 (MQ=255)
ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc < 1:703850/133‑1 (MQ=255)
aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg < 1:814457/140‑1 (MQ=255)
tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa > 2:122395/1‑131 (MQ=255)
tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa < 1:122395/131‑1 (MQ=255)
tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt > 1:660855/1‑140 (MQ=255)
ttttGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGc > 1:804059/1‑140 (MQ=255)
tttGATATCGCCTCGTTTGGTGCG‑A‑GGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa < 1:134371/140‑1 (MQ=255)
ttGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa < 2:660855/140‑1 (MQ=255)
gATATCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgg < 1:528193/140‑1 (MQ=255)
tatCGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg > 1:693906/1‑140 (MQ=255)
cGCCTCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCACCGTGGTTTTTGGTGCGAggggggg > 2:132429/1‑140 (MQ=255)
tCGTTTGGTGCG‑AGGGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAAAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggggac < 2:725218/140‑4 (MQ=255)
|
ATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACT > NC_000913/4606074‑4606333
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
