Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 128 54.7 1845674 99.3% 1832754 139.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,892,308 G→A G32E (GGG→GAG)  queD → 6‑carboxy‑5,6,7,8‑tetrahydropterin synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,892,3080GA100.0% 198.9 / NA 56G32E (GGG→GAG) queD6‑carboxy‑5,6,7,8‑tetrahydropterin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/31);  total (25/31)

TTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGAT  >  NC_000913/2892172‑2892441
                                                                                                                                        |                                                                                                                                     
ttCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCa                                                                                                                                    <  2:62063/140‑1 (MQ=255)
ttCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCa                                                                                                                                    <  1:366029/140‑1 (MQ=255)
 tCCGGTACTCTACGGCGGTTTTTTCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCAt                                                                                                                                   >  2:729476/1‑140 (MQ=255)
       aCTCTACGGCGGTTTTTTTGTCCTTTAGAGAAAATATGATGTCCACCACGTTATTTAAAAATTTCACCTTCCAAACCCCTCACCGCTTTCCACACGTCCCCGGAGGGCATAAAATTGGGCGCCCGCACGAGCATTCCttt                                                                                                                             >  2:381990/1‑140 (MQ=255)
             cggcggTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCCGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTg                                                                                                                       <  2:634852/140‑1 (MQ=255)
              ggcggTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGc                                                                                                                      <  2:871118/140‑1 (MQ=255)
                cggTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGa                                                                                                                    <  1:486461/140‑1 (MQ=255)
                       aTCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGaaa                                                                                                             >  1:82838/1‑140 (MQ=255)
                              tGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCgg                                                                                                      <  1:564224/140‑1 (MQ=255)
                               gTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCggg                                                                                                     >  2:531325/1‑140 (MQ=255)
                                     aaaTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTc                                                                                               >  1:273901/1‑140 (MQ=255)
                                     aaaTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTc                                                                                               >  1:514369/1‑140 (MQ=255)
                                     aaaTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTc                                                                                               >  2:166549/1‑140 (MQ=255)
                                     aaaTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTc                                                                                               >  2:494653/1‑140 (MQ=255)
                                       aTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGa                                                                                             >  1:2046/1‑140 (MQ=255)
                                          atgatgTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATcc                                                                                          >  1:515644/1‑140 (MQ=255)
                                          atgatgTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATcc                                                                                          >  1:542649/1‑140 (MQ=255)
                                             atgTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCa                                                                                       <  1:381990/140‑1 (MQ=255)
                                              tgTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCAt                                                                                      <  2:554382/140‑1 (MQ=255)
                                               gTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATc                                                                                           <  1:536262/134‑1 (MQ=255)
                                               gTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATc                                                                                           >  2:536262/1‑134 (MQ=255)
                                                tCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATAc                                                                                    >  1:186338/1‑140 (MQ=255)
                                                 ccaccaCGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACg                                                                                   <  1:919532/140‑1 (MQ=255)
                                                  caccacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACgg                                                                                  >  2:487416/1‑140 (MQ=255)
                                                  caccacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACgg                                                                                  >  1:542640/1‑140 (MQ=255)
                                                  caccacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACgg                                                                                  >  2:303701/1‑140 (MQ=255)
                                                     cacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCt                                                                               <  2:514369/140‑1 (MQ=255)
                                                        gTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGa                                                                            <  1:565962/140‑1 (MQ=255)
                                                          tATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGAtt                                                                          >  1:864924/1‑140 (MQ=255)
                                                            tttAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTAt                                                                        >  2:303225/1‑140 (MQ=255)
                                                                    tttCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCg                                                                <  2:551577/140‑1 (MQ=255)
                                                                     ttCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGc                                                               >  1:823485/1‑140 (MQ=255)
                                                                      tCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCt                                                              <  1:648634/140‑1 (MQ=255)
                                                                      tCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCt                                                              <  2:82838/140‑1 (MQ=255)
                                                                          cTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAAc                                                          <  1:143033/140‑1 (MQ=255)
                                                                                 gCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAgc                                                   <  1:555775/140‑1 (MQ=255)
                                                                                    gCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAgcggc                                                >  1:207103/1‑140 (MQ=255)
                                                                                        aCCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGttt                                            <  1:318743/140‑1 (MQ=255)
                                                                                          cGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATAATCGATTTCGCTGAACTAAAAGCGGCGTTTaa                                          <  2:355566/140‑1 (MQ=255)
                                                                                             ttACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAaacc                                       <  1:367626/140‑1 (MQ=255)
                                                                                                       tCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAg                             >  2:569984/1‑140 (MQ=255)
                                                                                                        cccGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAgc                            <  2:864924/140‑1 (MQ=255)
                                                                                                           ggAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGcc                         <  2:823485/140‑1 (MQ=255)
                                                                                                            gAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCt                        <  2:186338/140‑1 (MQ=255)
                                                                                                               gggCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGa                     <  1:729476/140‑1 (MQ=255)
                                                                                                               gggCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGa                     <  1:569984/140‑1 (MQ=255)
                                                                                                               gggCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGa                     <  2:2046/140‑1 (MQ=255)
                                                                                                                ggCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGAt                    <  2:515644/140‑1 (MQ=255)
                                                                                                                ggCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGAt                    >  1:518668/1‑140 (MQ=255)
                                                                                                                  cATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCa                  >  1:745713/1‑140 (MQ=255)
                                                                                                                     aaaTGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCGCCa               <  1:494653/140‑1 (MQ=255)
                                                                                                                       aTGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCAtt             <  1:303701/140‑1 (MQ=255)
                                                                                                                          tgGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTCTAAACCAACCTACGAGCGCCTCGATCACCATTAtc          >  2:752356/1‑140 (MQ=255)
                                                                                                                           ggTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTAtct         <  2:542640/140‑1 (MQ=255)
                                                                                                                               gCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAAt     >  2:100837/1‑140 (MQ=255)
                                                                                                                                 cTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGa   <  1:487416/140‑1 (MQ=255)
                                                                                                                                 cTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGa   >  1:145423/1‑140 (MQ=255)
                                                                                                                                  tGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGat  >  1:677816/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGAT  >  NC_000913/2892172‑2892441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A